Pericytes Stimulate Oligodendrocyte Progenitor Cell Differentiation during CNS Remyelination
The role of the neurovascular niche in CNS myelin regeneration is incompletely understood. Here, we show that, upon demyelination, CNS-resident pericytes (PCs) proliferate, and parenchymal non-vessel-associated PC-like cells (PLCs) rapidly develop. During remyelination, mature oligodendrocytes were...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2017-08-01
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| Series: | Cell Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124717310951 |
| _version_ | 1818265619756220416 |
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| author | Alerie Guzman De La Fuente Simona Lange Maria Elena Silva Ginez A. Gonzalez Herbert Tempfer Peter van Wijngaarden Chao Zhao Ludovica Di Canio Andrea Trost Lara Bieler Pia Zaunmair Peter Rotheneichner Anna O’Sullivan Sebastien Couillard-Despres Oihana Errea Maarja A. Mäe Johanna Andrae Liqun He Annika Keller Luis F. Bátiz Christer Betsholtz Ludwig Aigner Robin J.M. Franklin Francisco J. Rivera |
| author_facet | Alerie Guzman De La Fuente Simona Lange Maria Elena Silva Ginez A. Gonzalez Herbert Tempfer Peter van Wijngaarden Chao Zhao Ludovica Di Canio Andrea Trost Lara Bieler Pia Zaunmair Peter Rotheneichner Anna O’Sullivan Sebastien Couillard-Despres Oihana Errea Maarja A. Mäe Johanna Andrae Liqun He Annika Keller Luis F. Bátiz Christer Betsholtz Ludwig Aigner Robin J.M. Franklin Francisco J. Rivera |
| author_sort | Alerie Guzman De La Fuente |
| collection | DOAJ |
| description | The role of the neurovascular niche in CNS myelin regeneration is incompletely understood. Here, we show that, upon demyelination, CNS-resident pericytes (PCs) proliferate, and parenchymal non-vessel-associated PC-like cells (PLCs) rapidly develop. During remyelination, mature oligodendrocytes were found in close proximity to PCs. In Pdgfbret/ret mice, which have reduced PC numbers, oligodendrocyte progenitor cell (OPC) differentiation was delayed, although remyelination proceeded to completion. PC-conditioned medium accelerated and enhanced OPC differentiation in vitro and increased the rate of remyelination in an ex vivo cerebellar slice model of demyelination. We identified Lama2 as a PC-derived factor that promotes OPC differentiation. Thus, the functional role of PCs is not restricted to vascular homeostasis but includes the modulation of adult CNS progenitor cells involved in regeneration. |
| first_indexed | 2024-12-12T19:53:41Z |
| format | Article |
| id | doaj.art-4a4c8209548f4d78bf8a35c0c107082d |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-12T19:53:41Z |
| publishDate | 2017-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-4a4c8209548f4d78bf8a35c0c107082d2022-12-22T00:13:55ZengElsevierCell Reports2211-12472017-08-012081755176410.1016/j.celrep.2017.08.007Pericytes Stimulate Oligodendrocyte Progenitor Cell Differentiation during CNS RemyelinationAlerie Guzman De La Fuente0Simona Lange1Maria Elena Silva2Ginez A. Gonzalez3Herbert Tempfer4Peter van Wijngaarden5Chao Zhao6Ludovica Di Canio7Andrea Trost8Lara Bieler9Pia Zaunmair10Peter Rotheneichner11Anna O’Sullivan12Sebastien Couillard-Despres13Oihana Errea14Maarja A. Mäe15Johanna Andrae16Liqun He17Annika Keller18Luis F. Bátiz19Christer Betsholtz20Ludwig Aigner21Robin J.M. Franklin22Francisco J. Rivera23Wellcome Trust and MRC Cambridge Stem Cell Institute, University of Cambridge, Cambridge CB20AH, UKInstitute of Molecular Regenerative Medicine, Paracelsus Medical University Salzburg, 5020 Salzburg, AustriaWellcome Trust and MRC Cambridge Stem Cell Institute, University of Cambridge, Cambridge CB20AH, UKWellcome Trust and MRC Cambridge Stem Cell Institute, University of Cambridge, Cambridge CB20AH, UKSpinal Cord Injury and Tissue Regeneration Center Salzburg (SCI-TReCS), Paracelsus Medical University Salzburg, 5020 Salzburg, AustriaWellcome Trust and MRC Cambridge Stem Cell Institute, University of Cambridge, Cambridge CB20AH, UKWellcome Trust and MRC Cambridge Stem Cell Institute, University of Cambridge, Cambridge CB20AH, UKWellcome Trust and MRC Cambridge Stem Cell Institute, University of Cambridge, Cambridge CB20AH, UKInstitute of Molecular Regenerative Medicine, Paracelsus Medical University Salzburg, 5020 Salzburg, AustriaSpinal Cord Injury and Tissue Regeneration Center Salzburg (SCI-TReCS), Paracelsus Medical University Salzburg, 5020 Salzburg, AustriaSpinal Cord Injury and Tissue Regeneration Center Salzburg (SCI-TReCS), Paracelsus Medical University Salzburg, 5020 Salzburg, AustriaSpinal Cord Injury and Tissue Regeneration Center Salzburg (SCI-TReCS), Paracelsus Medical University Salzburg, 5020 Salzburg, AustriaSpinal Cord Injury and Tissue Regeneration Center Salzburg (SCI-TReCS), Paracelsus Medical University Salzburg, 5020 Salzburg, AustriaSpinal Cord Injury and Tissue Regeneration Center Salzburg (SCI-TReCS), Paracelsus Medical University Salzburg, 5020 Salzburg, AustriaWellcome Trust and MRC Cambridge Stem Cell Institute, University of Cambridge, Cambridge CB20AH, UKDepartment of Immunology, Genetics and Pathology, Rudbeck Laboratory, Uppsala University, 751 85 Uppsala, SwedenDepartment of Immunology, Genetics and Pathology, Rudbeck Laboratory, Uppsala University, 751 85 Uppsala, SwedenDepartment of Neurosurgery, Tianjin Medical University General Hospital, Tianjin Neurological Institute, Key Laboratory of Post-Neuroinjury Neuro-Repair and Regeneration in Central Nervous System, Ministry of Education and Tianjin City, Tianjin 300052, ChinaDivision of Neurosurgery, Zürich University Hospital, Zürich University, 8091 Zürich, SwitzerlandLaboratory of Stem Cells and Neuroregeneration, Institute of Anatomy, Histology and Pathology, Faculty of Medicine, Universidad Austral de Chile, Valdivia, ChileDepartment of Immunology, Genetics and Pathology, Rudbeck Laboratory, Uppsala University, 751 85 Uppsala, SwedenInstitute of Molecular Regenerative Medicine, Paracelsus Medical University Salzburg, 5020 Salzburg, AustriaWellcome Trust and MRC Cambridge Stem Cell Institute, University of Cambridge, Cambridge CB20AH, UKWellcome Trust and MRC Cambridge Stem Cell Institute, University of Cambridge, Cambridge CB20AH, UKThe role of the neurovascular niche in CNS myelin regeneration is incompletely understood. Here, we show that, upon demyelination, CNS-resident pericytes (PCs) proliferate, and parenchymal non-vessel-associated PC-like cells (PLCs) rapidly develop. During remyelination, mature oligodendrocytes were found in close proximity to PCs. In Pdgfbret/ret mice, which have reduced PC numbers, oligodendrocyte progenitor cell (OPC) differentiation was delayed, although remyelination proceeded to completion. PC-conditioned medium accelerated and enhanced OPC differentiation in vitro and increased the rate of remyelination in an ex vivo cerebellar slice model of demyelination. We identified Lama2 as a PC-derived factor that promotes OPC differentiation. Thus, the functional role of PCs is not restricted to vascular homeostasis but includes the modulation of adult CNS progenitor cells involved in regeneration.http://www.sciencedirect.com/science/article/pii/S2211124717310951neurovascular nichepericytesremyelinationoligodendrocyte progenitor cellLama2 |
| spellingShingle | Alerie Guzman De La Fuente Simona Lange Maria Elena Silva Ginez A. Gonzalez Herbert Tempfer Peter van Wijngaarden Chao Zhao Ludovica Di Canio Andrea Trost Lara Bieler Pia Zaunmair Peter Rotheneichner Anna O’Sullivan Sebastien Couillard-Despres Oihana Errea Maarja A. Mäe Johanna Andrae Liqun He Annika Keller Luis F. Bátiz Christer Betsholtz Ludwig Aigner Robin J.M. Franklin Francisco J. Rivera Pericytes Stimulate Oligodendrocyte Progenitor Cell Differentiation during CNS Remyelination Cell Reports neurovascular niche pericytes remyelination oligodendrocyte progenitor cell Lama2 |
| title | Pericytes Stimulate Oligodendrocyte Progenitor Cell Differentiation during CNS Remyelination |
| title_full | Pericytes Stimulate Oligodendrocyte Progenitor Cell Differentiation during CNS Remyelination |
| title_fullStr | Pericytes Stimulate Oligodendrocyte Progenitor Cell Differentiation during CNS Remyelination |
| title_full_unstemmed | Pericytes Stimulate Oligodendrocyte Progenitor Cell Differentiation during CNS Remyelination |
| title_short | Pericytes Stimulate Oligodendrocyte Progenitor Cell Differentiation during CNS Remyelination |
| title_sort | pericytes stimulate oligodendrocyte progenitor cell differentiation during cns remyelination |
| topic | neurovascular niche pericytes remyelination oligodendrocyte progenitor cell Lama2 |
| url | http://www.sciencedirect.com/science/article/pii/S2211124717310951 |
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