Fluorogenic Aptasensors with Small Molecules
Aptamers are single-stranded DNA or RNA molecules that can be identified through an iterative in vitro selection–amplification process. Among them, fluorogenic aptamers in response to small molecules have been of great interest in biosensing and bioimaging due to their rapid fluorescence turn-on sig...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-03-01
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| Series: | Chemosensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2227-9040/9/3/54 |
| _version_ | 1811153756977364992 |
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| author | Eun-Song Lee Jeong Min Lee Hea-Jin Kim Young-Pil Kim |
| author_facet | Eun-Song Lee Jeong Min Lee Hea-Jin Kim Young-Pil Kim |
| author_sort | Eun-Song Lee |
| collection | DOAJ |
| description | Aptamers are single-stranded DNA or RNA molecules that can be identified through an iterative in vitro selection–amplification process. Among them, fluorogenic aptamers in response to small molecules have been of great interest in biosensing and bioimaging due to their rapid fluorescence turn-on signals with high target specificity and low background noise. In this review, we report recent advances in fluorogenic aptasensors and their applications to in vitro diagnosis and cellular imaging. These aptasensors modulated by small molecules have been implemented in different modalities that include duplex or molecular beacon-type aptasensors, aptazymes, and fluorogen-activating aptamer reporters. We highlight the working principles, target molecules, modifications, and performance characteristics of fluorogenic aptasensors, and discuss their potential roles in the field of biosensor and bioimaging with future directions and challenges. |
| first_indexed | 2024-03-10T13:22:11Z |
| format | Article |
| id | doaj.art-4a4cedc092e4477c9a882f71076633b3 |
| institution | Directory Open Access Journal |
| issn | 2227-9040 |
| language | English |
| last_indexed | 2024-03-10T13:22:11Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Chemosensors |
| spelling | doaj.art-4a4cedc092e4477c9a882f71076633b32023-11-21T09:55:15ZengMDPI AGChemosensors2227-90402021-03-01935410.3390/chemosensors9030054Fluorogenic Aptasensors with Small MoleculesEun-Song Lee0Jeong Min Lee1Hea-Jin Kim2Young-Pil Kim3Department of Life Science, Hanyang University, Seoul 04763, KoreaDepartment of Life Science, Hanyang University, Seoul 04763, KoreaDepartment of Life Science, Hanyang University, Seoul 04763, KoreaDepartment of Life Science, Hanyang University, Seoul 04763, KoreaAptamers are single-stranded DNA or RNA molecules that can be identified through an iterative in vitro selection–amplification process. Among them, fluorogenic aptamers in response to small molecules have been of great interest in biosensing and bioimaging due to their rapid fluorescence turn-on signals with high target specificity and low background noise. In this review, we report recent advances in fluorogenic aptasensors and their applications to in vitro diagnosis and cellular imaging. These aptasensors modulated by small molecules have been implemented in different modalities that include duplex or molecular beacon-type aptasensors, aptazymes, and fluorogen-activating aptamer reporters. We highlight the working principles, target molecules, modifications, and performance characteristics of fluorogenic aptasensors, and discuss their potential roles in the field of biosensor and bioimaging with future directions and challenges.https://www.mdpi.com/2227-9040/9/3/54aptameraptasensoraptazymefluorogensmall moleculeRNA reporter |
| spellingShingle | Eun-Song Lee Jeong Min Lee Hea-Jin Kim Young-Pil Kim Fluorogenic Aptasensors with Small Molecules Chemosensors aptamer aptasensor aptazyme fluorogen small molecule RNA reporter |
| title | Fluorogenic Aptasensors with Small Molecules |
| title_full | Fluorogenic Aptasensors with Small Molecules |
| title_fullStr | Fluorogenic Aptasensors with Small Molecules |
| title_full_unstemmed | Fluorogenic Aptasensors with Small Molecules |
| title_short | Fluorogenic Aptasensors with Small Molecules |
| title_sort | fluorogenic aptasensors with small molecules |
| topic | aptamer aptasensor aptazyme fluorogen small molecule RNA reporter |
| url | https://www.mdpi.com/2227-9040/9/3/54 |
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