Tissue Culture and Refreshment Techniques for Improvement of Transformation in Local Tetraploid and Diploid Potato with Late Blight Resistance as an Example
Potato (<i>Solanum tuberosum</i>) is among the best producers of edible biomass in terms of yield per hectare and a variety of different regional cultivars are used as a staple commodity in many countries. However, this crop is attacked by several diseases, with the worst being the late...
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| Format: | Article |
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MDPI AG
2020-05-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/9/6/695 |
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| author | Eu Sheng Wang Nam Phuong Kieu Marit Lenman Erik Andreasson |
| author_facet | Eu Sheng Wang Nam Phuong Kieu Marit Lenman Erik Andreasson |
| author_sort | Eu Sheng Wang |
| collection | DOAJ |
| description | Potato (<i>Solanum tuberosum</i>) is among the best producers of edible biomass in terms of yield per hectare and a variety of different regional cultivars are used as a staple commodity in many countries. However, this crop is attacked by several diseases, with the worst being the late blight disease caused by <i>Phytophthora infestans</i>. Stacking of resistance (R) genes from wild <i>Solanum</i> relatives are interesting prospects for the sustainable control of late blight. Therefore, we optimized methods for the efficient generation and screening of R-gene-containing transformants in tetraploid and diploid hybrid potato genotypes. Using these methods, a high transformation efficiency was achieved for the transformation of tetraploid and diploid potato lines with a triple resistance (3R) gene construct. Transformation efficiencies were improved by optimizing several factors affecting regeneration, including the quality of the starting plant material, and the composition of the plant growth regulators used during selective regeneration. A refreshment protocol was designed to alleviate in vitro related stress in stock plants, which significantly improved the growth vigor and resulted in a 4- to 10-fold increase in transformation efficiency. Furthermore, long-term exposure to exogenous Indole-3-butyric acid that is usually used for the initiation of roots in vitro, was found to cause aberrant morphological phenotypes in potato. |
| first_indexed | 2024-03-10T19:29:31Z |
| format | Article |
| id | doaj.art-4a4db57da7774e31abf068e644cad121 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T19:29:31Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-4a4db57da7774e31abf068e644cad1212023-11-20T02:12:45ZengMDPI AGPlants2223-77472020-05-019669510.3390/plants9060695Tissue Culture and Refreshment Techniques for Improvement of Transformation in Local Tetraploid and Diploid Potato with Late Blight Resistance as an ExampleEu Sheng Wang0Nam Phuong Kieu1Marit Lenman2Erik Andreasson3Department of Plant Protection Biology, Swedish University of Agricultural Sciences, SE-230 53 Alnarp, SwedenDepartment of Plant Protection Biology, Swedish University of Agricultural Sciences, SE-230 53 Alnarp, SwedenDepartment of Plant Protection Biology, Swedish University of Agricultural Sciences, SE-230 53 Alnarp, SwedenDepartment of Plant Protection Biology, Swedish University of Agricultural Sciences, SE-230 53 Alnarp, SwedenPotato (<i>Solanum tuberosum</i>) is among the best producers of edible biomass in terms of yield per hectare and a variety of different regional cultivars are used as a staple commodity in many countries. However, this crop is attacked by several diseases, with the worst being the late blight disease caused by <i>Phytophthora infestans</i>. Stacking of resistance (R) genes from wild <i>Solanum</i> relatives are interesting prospects for the sustainable control of late blight. Therefore, we optimized methods for the efficient generation and screening of R-gene-containing transformants in tetraploid and diploid hybrid potato genotypes. Using these methods, a high transformation efficiency was achieved for the transformation of tetraploid and diploid potato lines with a triple resistance (3R) gene construct. Transformation efficiencies were improved by optimizing several factors affecting regeneration, including the quality of the starting plant material, and the composition of the plant growth regulators used during selective regeneration. A refreshment protocol was designed to alleviate in vitro related stress in stock plants, which significantly improved the growth vigor and resulted in a 4- to 10-fold increase in transformation efficiency. Furthermore, long-term exposure to exogenous Indole-3-butyric acid that is usually used for the initiation of roots in vitro, was found to cause aberrant morphological phenotypes in potato.https://www.mdpi.com/2223-7747/9/6/695resistance genes<i>Agrobacterium</i> transformationrecalcitrant<i>Phytophthora infestans</i>refreshment protocol |
| spellingShingle | Eu Sheng Wang Nam Phuong Kieu Marit Lenman Erik Andreasson Tissue Culture and Refreshment Techniques for Improvement of Transformation in Local Tetraploid and Diploid Potato with Late Blight Resistance as an Example Plants resistance genes <i>Agrobacterium</i> transformation recalcitrant <i>Phytophthora infestans</i> refreshment protocol |
| title | Tissue Culture and Refreshment Techniques for Improvement of Transformation in Local Tetraploid and Diploid Potato with Late Blight Resistance as an Example |
| title_full | Tissue Culture and Refreshment Techniques for Improvement of Transformation in Local Tetraploid and Diploid Potato with Late Blight Resistance as an Example |
| title_fullStr | Tissue Culture and Refreshment Techniques for Improvement of Transformation in Local Tetraploid and Diploid Potato with Late Blight Resistance as an Example |
| title_full_unstemmed | Tissue Culture and Refreshment Techniques for Improvement of Transformation in Local Tetraploid and Diploid Potato with Late Blight Resistance as an Example |
| title_short | Tissue Culture and Refreshment Techniques for Improvement of Transformation in Local Tetraploid and Diploid Potato with Late Blight Resistance as an Example |
| title_sort | tissue culture and refreshment techniques for improvement of transformation in local tetraploid and diploid potato with late blight resistance as an example |
| topic | resistance genes <i>Agrobacterium</i> transformation recalcitrant <i>Phytophthora infestans</i> refreshment protocol |
| url | https://www.mdpi.com/2223-7747/9/6/695 |
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