Pre and Post Dialysis Variations in Serum Lipid Profile among End Stage Renal Disease Patients
Introduction: Chronic Kidney Disease (CKD) is a devastating disease and 50% of CKD patients die from Cardiovascular Disease (CVD) rather than End Stage Renal Disease (ESRD). Dyslipidaemia is a major contributor of CVD in these patients. The exact effect of Renal Dialysis (RD) on lipid parameters in...
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JCDR Research and Publications Private Limited
2018-09-01
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| Series: | Journal of Clinical and Diagnostic Research |
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| Online Access: | https://jcdr.net/articles/PDF/11983/35432_230718_35432_CE[Ra]_F(Sh)_PF1(MJ_AP)_PFA(MJ_SL)_PB(MJ_AP)_PN(AP).pdf |
| _version_ | 1818031550966530048 |
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| author | Karra Madhu Latha Bhongir Aparna Varma |
| author_facet | Karra Madhu Latha Bhongir Aparna Varma |
| author_sort | Karra Madhu Latha |
| collection | DOAJ |
| description | Introduction: Chronic Kidney Disease (CKD) is a devastating disease and 50% of CKD patients die from Cardiovascular Disease (CVD) rather than End Stage Renal Disease (ESRD). Dyslipidaemia is a major contributor of CVD in these patients. The exact effect of Renal Dialysis (RD) on lipid parameters in ESRD is not clearly elucidated. Aim: To assess the variations in serum lipid profile and study the pattern of lipoproteins by agarose gel electrophoresis before and after RD. Materials and Methods: In this case-control study, 30 ESRD patients (15 male and 15 female) receiving maintenance renal dialysis were taken as cases and 30 age and sex matched healthy individuals were recruited as controls. Lipid profile parameters were estimated in Siemens automated analyser. Lipoprotein electrophoresis was done on Sebia Semi automated analyser. The data were analysed using GraphPad prism. Student’s t-test for normally distributed data, Mann-Whitney U test for nonnormally distributed data and ANOVA for comparison of more than two groups was done. A p-value <0.05 was considered statistically significant. Results: In ESRD cases there was a significant increase (p <0.001) in Total Cholesterol (TC), Low Density Lipoprotein Cholesterol (LDL-C), High Density Lipoprotein Cholesterol (HDL-C) after RD compared to the values before RD (151.4±38.92 versus 183±57.18 mg/dL; 75.3±24.66 mg/dL versus 84.23±29.74; 31.9±9.74 mg/dL versus 35.57±12.49 mg/dL); respectively. Triglycerides (TG) and Very Low Density Lipoprotein (VLDL) values decreased after RD compared to the values before RD (123.6±50 versus 121.4±49.26; 24.73±10 versus 24.29±9.85); (p>0.05). Fractionation of lipoproteins reveals a significant increase in lipoprotein a {Lp(a)} as compared to controls (21.20±17.46 versus 12.38±10.63, p<0.05). The values of alpha and beta lipoproteins in electrophoresis didn’t correlate well with the values obtained from automated enzymatic lipoprotein measurements in ESRD cases after dialysis (r=0.22, p=0.18). Conclusion: There is a remarkable increase in atherosclerotic risk after RD noted due to significant increase in TC, LDL-C and Lp(a) which might increase the risk of CVD in RD subjects. |
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| spelling | doaj.art-4a5160ed5c9144c2b96a721d17ab397d2022-12-22T01:59:59ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2018-09-01129BC05BC0910.7860/JCDR/2018/35432.11983Pre and Post Dialysis Variations in Serum Lipid Profile among End Stage Renal Disease PatientsKarra Madhu Latha0Bhongir Aparna Varma1Assistant Professor , Department of Biochemistry, Nizam’s Institute of Medical Sciences, Hyderabad, Telangana, India.Professor and Head, Department of Biochemistry, Mediciti Institute of Medical Sciences, Hyderabad, Telangana, India.Introduction: Chronic Kidney Disease (CKD) is a devastating disease and 50% of CKD patients die from Cardiovascular Disease (CVD) rather than End Stage Renal Disease (ESRD). Dyslipidaemia is a major contributor of CVD in these patients. The exact effect of Renal Dialysis (RD) on lipid parameters in ESRD is not clearly elucidated. Aim: To assess the variations in serum lipid profile and study the pattern of lipoproteins by agarose gel electrophoresis before and after RD. Materials and Methods: In this case-control study, 30 ESRD patients (15 male and 15 female) receiving maintenance renal dialysis were taken as cases and 30 age and sex matched healthy individuals were recruited as controls. Lipid profile parameters were estimated in Siemens automated analyser. Lipoprotein electrophoresis was done on Sebia Semi automated analyser. The data were analysed using GraphPad prism. Student’s t-test for normally distributed data, Mann-Whitney U test for nonnormally distributed data and ANOVA for comparison of more than two groups was done. A p-value <0.05 was considered statistically significant. Results: In ESRD cases there was a significant increase (p <0.001) in Total Cholesterol (TC), Low Density Lipoprotein Cholesterol (LDL-C), High Density Lipoprotein Cholesterol (HDL-C) after RD compared to the values before RD (151.4±38.92 versus 183±57.18 mg/dL; 75.3±24.66 mg/dL versus 84.23±29.74; 31.9±9.74 mg/dL versus 35.57±12.49 mg/dL); respectively. Triglycerides (TG) and Very Low Density Lipoprotein (VLDL) values decreased after RD compared to the values before RD (123.6±50 versus 121.4±49.26; 24.73±10 versus 24.29±9.85); (p>0.05). Fractionation of lipoproteins reveals a significant increase in lipoprotein a {Lp(a)} as compared to controls (21.20±17.46 versus 12.38±10.63, p<0.05). The values of alpha and beta lipoproteins in electrophoresis didn’t correlate well with the values obtained from automated enzymatic lipoprotein measurements in ESRD cases after dialysis (r=0.22, p=0.18). Conclusion: There is a remarkable increase in atherosclerotic risk after RD noted due to significant increase in TC, LDL-C and Lp(a) which might increase the risk of CVD in RD subjects.https://jcdr.net/articles/PDF/11983/35432_230718_35432_CE[Ra]_F(Sh)_PF1(MJ_AP)_PFA(MJ_SL)_PB(MJ_AP)_PN(AP).pdfcardiovascular diseasechronic kidney diseasedyslipidaemialipoprotein agarose gel electrophoresis |
| spellingShingle | Karra Madhu Latha Bhongir Aparna Varma Pre and Post Dialysis Variations in Serum Lipid Profile among End Stage Renal Disease Patients Journal of Clinical and Diagnostic Research cardiovascular disease chronic kidney disease dyslipidaemia lipoprotein agarose gel electrophoresis |
| title | Pre and Post Dialysis Variations in Serum Lipid Profile among End Stage Renal Disease Patients |
| title_full | Pre and Post Dialysis Variations in Serum Lipid Profile among End Stage Renal Disease Patients |
| title_fullStr | Pre and Post Dialysis Variations in Serum Lipid Profile among End Stage Renal Disease Patients |
| title_full_unstemmed | Pre and Post Dialysis Variations in Serum Lipid Profile among End Stage Renal Disease Patients |
| title_short | Pre and Post Dialysis Variations in Serum Lipid Profile among End Stage Renal Disease Patients |
| title_sort | pre and post dialysis variations in serum lipid profile among end stage renal disease patients |
| topic | cardiovascular disease chronic kidney disease dyslipidaemia lipoprotein agarose gel electrophoresis |
| url | https://jcdr.net/articles/PDF/11983/35432_230718_35432_CE[Ra]_F(Sh)_PF1(MJ_AP)_PFA(MJ_SL)_PB(MJ_AP)_PN(AP).pdf |
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