Zein-based 3D tubular constructs with tunable porosity for 3D cell culture and drug delivery
Manufacturing tubular constructs with tunable porosity can mimic the vascular structure, not only for supplying nutrients and removing metabolites to support long-term 3D cell culture but also for delivering bioactive components and drugs to tissues. There are few reports on the second purpose throu...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | Biomedical Engineering Advances |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667099222000354 |
| _version_ | 1797807170394259456 |
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| author | Ya-Qi Xue Yu-Cheng Zhang Yu-Bei Zhang Jin-Ye Wang |
| author_facet | Ya-Qi Xue Yu-Cheng Zhang Yu-Bei Zhang Jin-Ye Wang |
| author_sort | Ya-Qi Xue |
| collection | DOAJ |
| description | Manufacturing tubular constructs with tunable porosity can mimic the vascular structure, not only for supplying nutrients and removing metabolites to support long-term 3D cell culture but also for delivering bioactive components and drugs to tissues. There are few reports on the second purpose through 3D printing. In this study, bio-inspired tubular constructs with permeability were achieved using zein-based ink, forming structures with tunable porosity via the 3D printing technique. The parameters, e.g., zein content, with/without the addition of porogen, and drying conditions, were optimized to control the porous structure and porosity of the printed tubes. The inner wall of the resultant tube supported the adhesion of endothelial cells. A perfusion system was designed, and the penetrability of zein-based tubular constructs was demonstrated by the dialysis test. Moreover, perfusion of cell culture media and the anti-cancer drug in cell-laden hydrogels with tubular structure resulted in 3-day of 3D cell culture with a higher survival rate, and the drug was delivered to local cells around the tubular constructs, respectively. This is a new report on the preparation of 3D-printed tubular constructs using zein as the biomaterial inks with tunable porosity and porous structure, providing a general system for 3D cell culture, 3D drugs screening/pharmacokinetics in vitro, and tissue engineering. |
| first_indexed | 2024-03-13T06:18:32Z |
| format | Article |
| id | doaj.art-4a52ba513bde4808a1ed929b7769eb85 |
| institution | Directory Open Access Journal |
| issn | 2667-0992 |
| language | English |
| last_indexed | 2024-03-13T06:18:32Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biomedical Engineering Advances |
| spelling | doaj.art-4a52ba513bde4808a1ed929b7769eb852023-06-10T04:28:49ZengElsevierBiomedical Engineering Advances2667-09922023-06-015100059Zein-based 3D tubular constructs with tunable porosity for 3D cell culture and drug deliveryYa-Qi Xue0Yu-Cheng Zhang1Yu-Bei Zhang2Jin-Ye Wang3School of Biomedical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, ChinaSchool of Biomedical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, ChinaSchool of Biomedical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, ChinaSchool of Biomedical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, China; Corresponding author: School of Biomedical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, China.Manufacturing tubular constructs with tunable porosity can mimic the vascular structure, not only for supplying nutrients and removing metabolites to support long-term 3D cell culture but also for delivering bioactive components and drugs to tissues. There are few reports on the second purpose through 3D printing. In this study, bio-inspired tubular constructs with permeability were achieved using zein-based ink, forming structures with tunable porosity via the 3D printing technique. The parameters, e.g., zein content, with/without the addition of porogen, and drying conditions, were optimized to control the porous structure and porosity of the printed tubes. The inner wall of the resultant tube supported the adhesion of endothelial cells. A perfusion system was designed, and the penetrability of zein-based tubular constructs was demonstrated by the dialysis test. Moreover, perfusion of cell culture media and the anti-cancer drug in cell-laden hydrogels with tubular structure resulted in 3-day of 3D cell culture with a higher survival rate, and the drug was delivered to local cells around the tubular constructs, respectively. This is a new report on the preparation of 3D-printed tubular constructs using zein as the biomaterial inks with tunable porosity and porous structure, providing a general system for 3D cell culture, 3D drugs screening/pharmacokinetics in vitro, and tissue engineering.http://www.sciencedirect.com/science/article/pii/S2667099222000354Zein3D printingTubular constructs3D cell cultureDrug delivery |
| spellingShingle | Ya-Qi Xue Yu-Cheng Zhang Yu-Bei Zhang Jin-Ye Wang Zein-based 3D tubular constructs with tunable porosity for 3D cell culture and drug delivery Biomedical Engineering Advances Zein 3D printing Tubular constructs 3D cell culture Drug delivery |
| title | Zein-based 3D tubular constructs with tunable porosity for 3D cell culture and drug delivery |
| title_full | Zein-based 3D tubular constructs with tunable porosity for 3D cell culture and drug delivery |
| title_fullStr | Zein-based 3D tubular constructs with tunable porosity for 3D cell culture and drug delivery |
| title_full_unstemmed | Zein-based 3D tubular constructs with tunable porosity for 3D cell culture and drug delivery |
| title_short | Zein-based 3D tubular constructs with tunable porosity for 3D cell culture and drug delivery |
| title_sort | zein based 3d tubular constructs with tunable porosity for 3d cell culture and drug delivery |
| topic | Zein 3D printing Tubular constructs 3D cell culture Drug delivery |
| url | http://www.sciencedirect.com/science/article/pii/S2667099222000354 |
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