Combined Yeast Cultivation and Pectin Hydrolysis as an Effective Method of Producing Prebiotic Animal Feed from Sugar Beet Pulp
An effective and ecological method for liberation of pectin-derived oligosaccharides (POS) from sugar beet pulp (SBP) was developed using enzymatic and microorganism-mediated biomass conversion. The POS may be applied in the production of prebiotic feed additives. Various yeast strains were screened...
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MDPI AG
2020-05-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/10/5/724 |
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| author | Agnieszka Wilkowska Joanna Berlowska Adriana Nowak Ilona Motyl Aneta Antczak-Chrobot Maciej Wojtczak Alina Kunicka-Styczyńska Michał Binczarski Piotr Dziugan |
| author_facet | Agnieszka Wilkowska Joanna Berlowska Adriana Nowak Ilona Motyl Aneta Antczak-Chrobot Maciej Wojtczak Alina Kunicka-Styczyńska Michał Binczarski Piotr Dziugan |
| author_sort | Agnieszka Wilkowska |
| collection | DOAJ |
| description | An effective and ecological method for liberation of pectin-derived oligosaccharides (POS) from sugar beet pulp (SBP) was developed using enzymatic and microorganism-mediated biomass conversion. The POS may be applied in the production of prebiotic feed additives. Various yeast strains were screened for their capacity for protein synthesis and monosaccharide assimilation. Combined yeast cultivation and pectin hydrolysis were found to be an effective method of producing prebiotics. Separate enzymatic hydrolysis and fermentation of SBP resulted in the release of 3.6 g of POS per 100 g d.w., whereas the yield of POS acquired after the combined process was 17.9% higher, giving 4.2 g of POS per 100 g d.w. Introducing the yeast into the process improved hydrolysis performance due to lower enzyme inhibition by mono- and disaccharides. The prebiotic effect of the POS was assessed by <i>in vitro</i> fermentation using individual cultures of gastrointestinal bacteria. The POS in the SBP hydrolysate effectively promoted the growth of lactobacilli and bifidobacteria. A large increase in adherence to Caco-2 cells in the presence of POS was noted for beneficial <i>Lactobacillus brevis</i> strains, whereas pathogenic bacteria and yeast (<i>C. albicans, C. lusitanie, C. pelliculosa</i>), responsible for infections in breeding animals, showed much weaker adhesion. |
| first_indexed | 2024-03-10T20:00:12Z |
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| id | doaj.art-4a554f23ec064760b972dd7fa1e0f1ee |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T20:00:12Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-4a554f23ec064760b972dd7fa1e0f1ee2023-11-19T23:37:45ZengMDPI AGBiomolecules2218-273X2020-05-0110572410.3390/biom10050724Combined Yeast Cultivation and Pectin Hydrolysis as an Effective Method of Producing Prebiotic Animal Feed from Sugar Beet PulpAgnieszka Wilkowska0Joanna Berlowska1Adriana Nowak2Ilona Motyl3Aneta Antczak-Chrobot4Maciej Wojtczak5Alina Kunicka-Styczyńska6Michał Binczarski7Piotr Dziugan8Institute of Fermentation Technology and Microbiology, Faculty of Biotechnology and Food Science, Łódź University of Technology, Wólczańska 171/173, 90-924 Łódź, PolandDepartment of Environmental Biotechnology, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Wólczańska 171/173, 90-924 Łódź, PolandDepartment of Environmental Biotechnology, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Wólczańska 171/173, 90-924 Łódź, PolandDepartment of Environmental Biotechnology, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Wólczańska 171/173, 90-924 Łódź, PolandInstitute of Technology and Analysis of Food, Faculty of Biotechnology and Food Science, Lodz University of Technology, Wólczańska 171/173, 90-924 Łódź, PolandInstitute of Technology and Analysis of Food, Faculty of Biotechnology and Food Science, Lodz University of Technology, Wólczańska 171/173, 90-924 Łódź, PolandInstitute of Fermentation Technology and Microbiology, Faculty of Biotechnology and Food Science, Łódź University of Technology, Wólczańska 171/173, 90-924 Łódź, PolandInstitute of General and Ecological Chemistry, Faculty of Chemistry, Lodz University of Technology, Żeromskiego 116, 90-924 Łódź, PolandDepartment of Environmental Biotechnology, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Wólczańska 171/173, 90-924 Łódź, PolandAn effective and ecological method for liberation of pectin-derived oligosaccharides (POS) from sugar beet pulp (SBP) was developed using enzymatic and microorganism-mediated biomass conversion. The POS may be applied in the production of prebiotic feed additives. Various yeast strains were screened for their capacity for protein synthesis and monosaccharide assimilation. Combined yeast cultivation and pectin hydrolysis were found to be an effective method of producing prebiotics. Separate enzymatic hydrolysis and fermentation of SBP resulted in the release of 3.6 g of POS per 100 g d.w., whereas the yield of POS acquired after the combined process was 17.9% higher, giving 4.2 g of POS per 100 g d.w. Introducing the yeast into the process improved hydrolysis performance due to lower enzyme inhibition by mono- and disaccharides. The prebiotic effect of the POS was assessed by <i>in vitro</i> fermentation using individual cultures of gastrointestinal bacteria. The POS in the SBP hydrolysate effectively promoted the growth of lactobacilli and bifidobacteria. A large increase in adherence to Caco-2 cells in the presence of POS was noted for beneficial <i>Lactobacillus brevis</i> strains, whereas pathogenic bacteria and yeast (<i>C. albicans, C. lusitanie, C. pelliculosa</i>), responsible for infections in breeding animals, showed much weaker adhesion.https://www.mdpi.com/2218-273X/10/5/724pectin-derived oligosaccharides (POS)prebioticssugar beet pulpanimal feed |
| spellingShingle | Agnieszka Wilkowska Joanna Berlowska Adriana Nowak Ilona Motyl Aneta Antczak-Chrobot Maciej Wojtczak Alina Kunicka-Styczyńska Michał Binczarski Piotr Dziugan Combined Yeast Cultivation and Pectin Hydrolysis as an Effective Method of Producing Prebiotic Animal Feed from Sugar Beet Pulp Biomolecules pectin-derived oligosaccharides (POS) prebiotics sugar beet pulp animal feed |
| title | Combined Yeast Cultivation and Pectin Hydrolysis as an Effective Method of Producing Prebiotic Animal Feed from Sugar Beet Pulp |
| title_full | Combined Yeast Cultivation and Pectin Hydrolysis as an Effective Method of Producing Prebiotic Animal Feed from Sugar Beet Pulp |
| title_fullStr | Combined Yeast Cultivation and Pectin Hydrolysis as an Effective Method of Producing Prebiotic Animal Feed from Sugar Beet Pulp |
| title_full_unstemmed | Combined Yeast Cultivation and Pectin Hydrolysis as an Effective Method of Producing Prebiotic Animal Feed from Sugar Beet Pulp |
| title_short | Combined Yeast Cultivation and Pectin Hydrolysis as an Effective Method of Producing Prebiotic Animal Feed from Sugar Beet Pulp |
| title_sort | combined yeast cultivation and pectin hydrolysis as an effective method of producing prebiotic animal feed from sugar beet pulp |
| topic | pectin-derived oligosaccharides (POS) prebiotics sugar beet pulp animal feed |
| url | https://www.mdpi.com/2218-273X/10/5/724 |
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