Disturbance in Some Fertility Biomarkers Induced and Changes in Testis Architecture by Chronic Exposure to Various Dosages of Each of Nonylphenol or Bisphenol A and Their Mix
This investigation was conducted to demonstrate the potential impacts of different doses of Bisphenol A (BPA) or Nonylphenol (NP) and their mixtures on some biological activities in male albino rats. Seventy male albino rats were allocated to the control group (GI) and were given 1 mL of ethanol. G...
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MDPI AG
2022-10-01
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| author | Sahar J. Melebary Mariam S. AlGhamdi Manal E. A. Elhalwagy Soha A. Alsolmy Al Jawaher A. Bin Dohaish |
| author_facet | Sahar J. Melebary Mariam S. AlGhamdi Manal E. A. Elhalwagy Soha A. Alsolmy Al Jawaher A. Bin Dohaish |
| author_sort | Sahar J. Melebary |
| collection | DOAJ |
| description | This investigation was conducted to demonstrate the potential impacts of different doses of Bisphenol A (BPA) or Nonylphenol (NP) and their mixtures on some biological activities in male albino rats. Seventy male albino rats were allocated to the control group (GI) and were given 1 mL of ethanol. G II and G III were given 100 mg/kg of each of BPA and NP, G IV and G V were given 25 mg/kg of each of BPA and NP, G VI was given a high dose of BPA and NP, and G VII was given a low dose of BPA and NP. All animals were treated orally for 60 days. Serum biomarkers of oxidative stress, antioxidants, immune-inflammatory mediators, and apoptotic markers were determined, as well as a histopathological examination of the testis at the end of the experimental period. The results obtained showed a pronounced increase in malondialdehyde (MDA), protein carbonyl (PC), and 4-hydroxynonenol (4-HNE), concomitant with a significant reduction in serum Superoxide dismutase (SOD), catalase enzyme (CAT), and total antioxidant capacity (TAC) in all treated groups. A significant elevation in TNF Alpha, TNF Beta, and Caspase 3 serum was recorded individually and in the groups treated with high doses. The disturbance is represented by histological damage in the testis in the germinal epithelium and a decrease in spermatozoa inside the lumen of seminiferous tubules. The effects on testis tissues were dose-dependent, pronounced in mixture doses, and remarkable in higher doses. In conclusion, exposure to BPA and NP strongly impacts antioxidants, immune-inflammatory mediators, and testis tissue architecture. Furthermore, the data from this investigation support the idea that exposure to BPA and NP in daily life has multiple damages. |
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| last_indexed | 2024-03-09T12:12:43Z |
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| series | Life |
| spelling | doaj.art-4a599f85431f4a1b8c36790c0813c5a92023-11-30T22:50:26ZengMDPI AGLife2075-17292022-10-011210155510.3390/life12101555Disturbance in Some Fertility Biomarkers Induced and Changes in Testis Architecture by Chronic Exposure to Various Dosages of Each of Nonylphenol or Bisphenol A and Their MixSahar J. Melebary0Mariam S. AlGhamdi1Manal E. A. Elhalwagy2Soha A. Alsolmy3Al Jawaher A. Bin Dohaish4Department of Biology, College of Science, University of Jeddah, Jeddah 21493, Saudi ArabiaDepartment of Biology, College of Science, University of Jeddah, Jeddah 21493, Saudi ArabiaDepartment of Biochemistry, College of Science, University of Jeddah, Jeddah 21493, Saudi ArabiaDepartment of Biology, College of Science, University of Jeddah, Jeddah 21493, Saudi ArabiaDepartment of Biology, College of Science, University of Jeddah, Jeddah 21493, Saudi ArabiaThis investigation was conducted to demonstrate the potential impacts of different doses of Bisphenol A (BPA) or Nonylphenol (NP) and their mixtures on some biological activities in male albino rats. Seventy male albino rats were allocated to the control group (GI) and were given 1 mL of ethanol. G II and G III were given 100 mg/kg of each of BPA and NP, G IV and G V were given 25 mg/kg of each of BPA and NP, G VI was given a high dose of BPA and NP, and G VII was given a low dose of BPA and NP. All animals were treated orally for 60 days. Serum biomarkers of oxidative stress, antioxidants, immune-inflammatory mediators, and apoptotic markers were determined, as well as a histopathological examination of the testis at the end of the experimental period. The results obtained showed a pronounced increase in malondialdehyde (MDA), protein carbonyl (PC), and 4-hydroxynonenol (4-HNE), concomitant with a significant reduction in serum Superoxide dismutase (SOD), catalase enzyme (CAT), and total antioxidant capacity (TAC) in all treated groups. A significant elevation in TNF Alpha, TNF Beta, and Caspase 3 serum was recorded individually and in the groups treated with high doses. The disturbance is represented by histological damage in the testis in the germinal epithelium and a decrease in spermatozoa inside the lumen of seminiferous tubules. The effects on testis tissues were dose-dependent, pronounced in mixture doses, and remarkable in higher doses. In conclusion, exposure to BPA and NP strongly impacts antioxidants, immune-inflammatory mediators, and testis tissue architecture. Furthermore, the data from this investigation support the idea that exposure to BPA and NP in daily life has multiple damages.https://www.mdpi.com/2075-1729/12/10/1555Bisphenol ANonylphenoloxidative stress markersantioxidant markerstestis biomarkersinflammatory markers |
| spellingShingle | Sahar J. Melebary Mariam S. AlGhamdi Manal E. A. Elhalwagy Soha A. Alsolmy Al Jawaher A. Bin Dohaish Disturbance in Some Fertility Biomarkers Induced and Changes in Testis Architecture by Chronic Exposure to Various Dosages of Each of Nonylphenol or Bisphenol A and Their Mix Life Bisphenol A Nonylphenol oxidative stress markers antioxidant markers testis biomarkers inflammatory markers |
| title | Disturbance in Some Fertility Biomarkers Induced and Changes in Testis Architecture by Chronic Exposure to Various Dosages of Each of Nonylphenol or Bisphenol A and Their Mix |
| title_full | Disturbance in Some Fertility Biomarkers Induced and Changes in Testis Architecture by Chronic Exposure to Various Dosages of Each of Nonylphenol or Bisphenol A and Their Mix |
| title_fullStr | Disturbance in Some Fertility Biomarkers Induced and Changes in Testis Architecture by Chronic Exposure to Various Dosages of Each of Nonylphenol or Bisphenol A and Their Mix |
| title_full_unstemmed | Disturbance in Some Fertility Biomarkers Induced and Changes in Testis Architecture by Chronic Exposure to Various Dosages of Each of Nonylphenol or Bisphenol A and Their Mix |
| title_short | Disturbance in Some Fertility Biomarkers Induced and Changes in Testis Architecture by Chronic Exposure to Various Dosages of Each of Nonylphenol or Bisphenol A and Their Mix |
| title_sort | disturbance in some fertility biomarkers induced and changes in testis architecture by chronic exposure to various dosages of each of nonylphenol or bisphenol a and their mix |
| topic | Bisphenol A Nonylphenol oxidative stress markers antioxidant markers testis biomarkers inflammatory markers |
| url | https://www.mdpi.com/2075-1729/12/10/1555 |
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