Environmental and Circadian Regulation Combine to Shape the Rhythmic Selenoproteome
The circadian clock orchestrates an organism’s endogenous processes with environmental 24 h cycles. Redox homeostasis and the circadian clock regulate one another to negate the potential effects of our planet’s light/dark cycle on the generation of reactive oxygen species (ROS) and attain homeostasi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-01-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/11/3/340 |
| _version_ | 1827661202413584384 |
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| author | Holly Kay Harry Taylor Gerben van Ooijen |
| author_facet | Holly Kay Harry Taylor Gerben van Ooijen |
| author_sort | Holly Kay |
| collection | DOAJ |
| description | The circadian clock orchestrates an organism’s endogenous processes with environmental 24 h cycles. Redox homeostasis and the circadian clock regulate one another to negate the potential effects of our planet’s light/dark cycle on the generation of reactive oxygen species (ROS) and attain homeostasis. Selenoproteins are an important class of redox-related enzymes that have a selenocysteine residue in the active site. This study reports functional understanding of how environmental and endogenous circadian rhythms integrate to shape the selenoproteome in a model eukaryotic cell. We mined quantitative proteomic data for the 24 selenoproteins of the picoeukaryote <i>Ostreococcus tauri</i> across time series, under environmentally rhythmic entrained conditions of light/dark (LD) cycles, compared to constant circadian conditions of constant light (LL). We found an overrepresentation of selenoproteins among rhythmic proteins under LL, but an underrepresentation under LD conditions. Rhythmic selenoproteins under LL that reach peak abundance later in the day showed a greater relative amplitude of oscillations than those that peak early in the day. Under LD, amplitude did not correlate with peak phase; however, we identified high-amplitude selenium uptake rhythms under LD but not LL conditions. Selenium deprivation induced strong qualitative defects in clock gene expression under LD but not LL conditions. Overall, the clear conclusion is that the circadian and environmental cycles exert differential effects on the selenoproteome, and that the combination of the two enables homeostasis. Selenoproteins may therefore play an important role in the cellular response to reactive oxygen species that form as a consequence of the transitions between light and dark. |
| first_indexed | 2024-03-10T00:05:11Z |
| format | Article |
| id | doaj.art-4a5a23d30b77469f8099f0b7cf5b51be |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T00:05:11Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-4a5a23d30b77469f8099f0b7cf5b51be2023-11-23T16:10:15ZengMDPI AGCells2073-44092022-01-0111334010.3390/cells11030340Environmental and Circadian Regulation Combine to Shape the Rhythmic SelenoproteomeHolly Kay0Harry Taylor1Gerben van Ooijen2School of Biological Sciences, University of Edinburgh, Max Born Crescent, Edinburgh EH9 3BF, UKSchool of Biological Sciences, University of Edinburgh, Max Born Crescent, Edinburgh EH9 3BF, UKSchool of Biological Sciences, University of Edinburgh, Max Born Crescent, Edinburgh EH9 3BF, UKThe circadian clock orchestrates an organism’s endogenous processes with environmental 24 h cycles. Redox homeostasis and the circadian clock regulate one another to negate the potential effects of our planet’s light/dark cycle on the generation of reactive oxygen species (ROS) and attain homeostasis. Selenoproteins are an important class of redox-related enzymes that have a selenocysteine residue in the active site. This study reports functional understanding of how environmental and endogenous circadian rhythms integrate to shape the selenoproteome in a model eukaryotic cell. We mined quantitative proteomic data for the 24 selenoproteins of the picoeukaryote <i>Ostreococcus tauri</i> across time series, under environmentally rhythmic entrained conditions of light/dark (LD) cycles, compared to constant circadian conditions of constant light (LL). We found an overrepresentation of selenoproteins among rhythmic proteins under LL, but an underrepresentation under LD conditions. Rhythmic selenoproteins under LL that reach peak abundance later in the day showed a greater relative amplitude of oscillations than those that peak early in the day. Under LD, amplitude did not correlate with peak phase; however, we identified high-amplitude selenium uptake rhythms under LD but not LL conditions. Selenium deprivation induced strong qualitative defects in clock gene expression under LD but not LL conditions. Overall, the clear conclusion is that the circadian and environmental cycles exert differential effects on the selenoproteome, and that the combination of the two enables homeostasis. Selenoproteins may therefore play an important role in the cellular response to reactive oxygen species that form as a consequence of the transitions between light and dark.https://www.mdpi.com/2073-4409/11/3/340selenocysteinecircadian clockselenoproteomeseleniumcellular rhythms |
| spellingShingle | Holly Kay Harry Taylor Gerben van Ooijen Environmental and Circadian Regulation Combine to Shape the Rhythmic Selenoproteome Cells selenocysteine circadian clock selenoproteome selenium cellular rhythms |
| title | Environmental and Circadian Regulation Combine to Shape the Rhythmic Selenoproteome |
| title_full | Environmental and Circadian Regulation Combine to Shape the Rhythmic Selenoproteome |
| title_fullStr | Environmental and Circadian Regulation Combine to Shape the Rhythmic Selenoproteome |
| title_full_unstemmed | Environmental and Circadian Regulation Combine to Shape the Rhythmic Selenoproteome |
| title_short | Environmental and Circadian Regulation Combine to Shape the Rhythmic Selenoproteome |
| title_sort | environmental and circadian regulation combine to shape the rhythmic selenoproteome |
| topic | selenocysteine circadian clock selenoproteome selenium cellular rhythms |
| url | https://www.mdpi.com/2073-4409/11/3/340 |
| work_keys_str_mv | AT hollykay environmentalandcircadianregulationcombinetoshapetherhythmicselenoproteome AT harrytaylor environmentalandcircadianregulationcombinetoshapetherhythmicselenoproteome AT gerbenvanooijen environmentalandcircadianregulationcombinetoshapetherhythmicselenoproteome |