Chemical stabilization of porous silicon for enhanced biofunctionalization with immunoglobulin
Porous silicon (PSi) is widely used in biological experiments, owing to its biocompatibility and well-established fabrication methods that allow tailoring its surface. Nevertheless, there are some unresolved issues such as deciding whether the stabilization of PSi is necessary for its biological app...
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2012-01-01
|
| Series: | Science and Technology of Advanced Materials |
| Online Access: | http://dx.doi.org/10.1088/1468-6996/13/4/045009 |
| _version_ | 1818050446503182336 |
|---|---|
| author | Nelson Naveas, Vicente Torres Costa, Dario Gallach, Jacobo Hernandez-Montelongo, Raul Jose Martín Palma, Josefa Predenstinacion Garcia-Ruiz and Miguel Manso-Silván |
| author_facet | Nelson Naveas, Vicente Torres Costa, Dario Gallach, Jacobo Hernandez-Montelongo, Raul Jose Martín Palma, Josefa Predenstinacion Garcia-Ruiz and Miguel Manso-Silván |
| author_sort | Nelson Naveas, Vicente Torres Costa, Dario Gallach, Jacobo Hernandez-Montelongo, Raul Jose Martín Palma, Josefa Predenstinacion Garcia-Ruiz and Miguel Manso-Silván |
| collection | DOAJ |
| description | Porous silicon (PSi) is widely used in biological experiments, owing to its biocompatibility and well-established fabrication methods that allow tailoring its surface. Nevertheless, there are some unresolved issues such as deciding whether the stabilization of PSi is necessary for its biological applications and evaluating the effects of PSi stabilization on the surface biofunctionalization with proteins. In this work we demonstrate that non-stabilized PSi is prone to detachment owing to the stress induced upon biomolecular adsorption. Biofunctionalized non-stabilized PSi loses the interference properties characteristic of a thin film, and groove-like structures resulting from a final layer collapse were observed by scanning electron microscopy. Likewise, direct PSi derivatization with 3-aminopropyl-triethoxysilane (APTS) does not stabilize PSi against immunoglobulin biofunctionalization. To overcome this problem, we developed a simple chemical process of stabilizing PSi (CoxPSi) for biological applications, which has several advantages over thermal stabilization (ToxPSi). The process consists of chemical oxidation in H2O2, surface derivatization with APTS and a curing step at 120 °C. This process offers integral homogeneous PSi morphology, hydrophilic surface termination (contact angle θ = 26°) and highly efficient derivatized and biofunctionalized PSi surfaces (six times more efficient than ToxPSi). All these features are highly desirable for biological applications, such as biosensing, where our results can be used for the design and optimization of the biomolecular immobilization cascade on PSi surfaces. |
| first_indexed | 2024-12-10T10:53:36Z |
| format | Article |
| id | doaj.art-4a5c8fc0fd8c448aa0b709d8197e155c |
| institution | Directory Open Access Journal |
| issn | 1468-6996 1878-5514 |
| language | English |
| last_indexed | 2024-12-10T10:53:36Z |
| publishDate | 2012-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Science and Technology of Advanced Materials |
| spelling | doaj.art-4a5c8fc0fd8c448aa0b709d8197e155c2022-12-22T01:51:56ZengTaylor & Francis GroupScience and Technology of Advanced Materials1468-69961878-55142012-01-01134045009Chemical stabilization of porous silicon for enhanced biofunctionalization with immunoglobulinNelson Naveas, Vicente Torres Costa, Dario Gallach, Jacobo Hernandez-Montelongo, Raul Jose Martín Palma, Josefa Predenstinacion Garcia-Ruiz and Miguel Manso-SilvánPorous silicon (PSi) is widely used in biological experiments, owing to its biocompatibility and well-established fabrication methods that allow tailoring its surface. Nevertheless, there are some unresolved issues such as deciding whether the stabilization of PSi is necessary for its biological applications and evaluating the effects of PSi stabilization on the surface biofunctionalization with proteins. In this work we demonstrate that non-stabilized PSi is prone to detachment owing to the stress induced upon biomolecular adsorption. Biofunctionalized non-stabilized PSi loses the interference properties characteristic of a thin film, and groove-like structures resulting from a final layer collapse were observed by scanning electron microscopy. Likewise, direct PSi derivatization with 3-aminopropyl-triethoxysilane (APTS) does not stabilize PSi against immunoglobulin biofunctionalization. To overcome this problem, we developed a simple chemical process of stabilizing PSi (CoxPSi) for biological applications, which has several advantages over thermal stabilization (ToxPSi). The process consists of chemical oxidation in H2O2, surface derivatization with APTS and a curing step at 120 °C. This process offers integral homogeneous PSi morphology, hydrophilic surface termination (contact angle θ = 26°) and highly efficient derivatized and biofunctionalized PSi surfaces (six times more efficient than ToxPSi). All these features are highly desirable for biological applications, such as biosensing, where our results can be used for the design and optimization of the biomolecular immobilization cascade on PSi surfaces.http://dx.doi.org/10.1088/1468-6996/13/4/045009 |
| spellingShingle | Nelson Naveas, Vicente Torres Costa, Dario Gallach, Jacobo Hernandez-Montelongo, Raul Jose Martín Palma, Josefa Predenstinacion Garcia-Ruiz and Miguel Manso-Silván Chemical stabilization of porous silicon for enhanced biofunctionalization with immunoglobulin Science and Technology of Advanced Materials |
| title | Chemical stabilization of porous silicon for enhanced biofunctionalization with immunoglobulin |
| title_full | Chemical stabilization of porous silicon for enhanced biofunctionalization with immunoglobulin |
| title_fullStr | Chemical stabilization of porous silicon for enhanced biofunctionalization with immunoglobulin |
| title_full_unstemmed | Chemical stabilization of porous silicon for enhanced biofunctionalization with immunoglobulin |
| title_short | Chemical stabilization of porous silicon for enhanced biofunctionalization with immunoglobulin |
| title_sort | chemical stabilization of porous silicon for enhanced biofunctionalization with immunoglobulin |
| url | http://dx.doi.org/10.1088/1468-6996/13/4/045009 |
| work_keys_str_mv | AT nelsonnaveasvicentetorrescostadariogallachjacobohernandezmontelongorauljosemartinpalmajosefapredenstinaciongarciaruizandmiguelmansosilvan chemicalstabilizationofporoussiliconforenhancedbiofunctionalizationwithimmunoglobulin |