Development of a Fully Automated Flow Injection Analyzer Implementing Bioluminescent Biosensors for Water Toxicity Assessment
This paper describes the development of an automated Flow Injection analyzer for water toxicity assessment. The analyzer is validated by assessing the toxicity of heavy metal (Pb2+, Hg2+ and Cu2+) solutions. One hundred μL of a Vibrio fischeri suspension are injected in a carrier solution containing...
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| Format: | Article |
| Language: | English |
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MDPI AG
2010-07-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/10/8/7089/ |
| _version_ | 1798025897791455232 |
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| author | Constantinos Georgiou Dimitrios G. Georgakopoulos Gerasimos Kremmydas Efstathios Vasiliou Efstratios Komaitis |
| author_facet | Constantinos Georgiou Dimitrios G. Georgakopoulos Gerasimos Kremmydas Efstathios Vasiliou Efstratios Komaitis |
| author_sort | Constantinos Georgiou |
| collection | DOAJ |
| description | This paper describes the development of an automated Flow Injection analyzer for water toxicity assessment. The analyzer is validated by assessing the toxicity of heavy metal (Pb2+, Hg2+ and Cu2+) solutions. One hundred μL of a Vibrio fischeri suspension are injected in a carrier solution containing different heavy metal concentrations. Biosensor cells are mixed with the toxic carrier solution in the mixing coil on the way to the detector. Response registered is % inhibition of biosensor bioluminescence due to heavy metal toxicity in comparison to that resulting by injecting the Vibrio fischeri suspension in deionised water. Carrier solutions of mercury showed higher toxicity than the other heavy metals, whereas all metals show concentration related levels of toxicity. The biosensor’s response to carrier solutions of different pHs was tested. Vibrio fischeri’s bioluminescence is promoted in the pH 5–10 range. Experiments indicate that the whole cell biosensor, as applied in the automated fluidic system, responds to various toxic solutions. |
| first_indexed | 2024-04-11T18:27:29Z |
| format | Article |
| id | doaj.art-4a5f8985e4ff4a49af66bc454573fd8d |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T18:27:29Z |
| publishDate | 2010-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-4a5f8985e4ff4a49af66bc454573fd8d2022-12-22T04:09:35ZengMDPI AGSensors1424-82202010-07-011087089709810.3390/s100807089Development of a Fully Automated Flow Injection Analyzer Implementing Bioluminescent Biosensors for Water Toxicity AssessmentConstantinos GeorgiouDimitrios G. GeorgakopoulosGerasimos KremmydasEfstathios VasiliouEfstratios KomaitisThis paper describes the development of an automated Flow Injection analyzer for water toxicity assessment. The analyzer is validated by assessing the toxicity of heavy metal (Pb2+, Hg2+ and Cu2+) solutions. One hundred μL of a Vibrio fischeri suspension are injected in a carrier solution containing different heavy metal concentrations. Biosensor cells are mixed with the toxic carrier solution in the mixing coil on the way to the detector. Response registered is % inhibition of biosensor bioluminescence due to heavy metal toxicity in comparison to that resulting by injecting the Vibrio fischeri suspension in deionised water. Carrier solutions of mercury showed higher toxicity than the other heavy metals, whereas all metals show concentration related levels of toxicity. The biosensor’s response to carrier solutions of different pHs was tested. Vibrio fischeri’s bioluminescence is promoted in the pH 5–10 range. Experiments indicate that the whole cell biosensor, as applied in the automated fluidic system, responds to various toxic solutions.http://www.mdpi.com/1424-8220/10/8/7089/Vibrio fischeritoxicityflow injection |
| spellingShingle | Constantinos Georgiou Dimitrios G. Georgakopoulos Gerasimos Kremmydas Efstathios Vasiliou Efstratios Komaitis Development of a Fully Automated Flow Injection Analyzer Implementing Bioluminescent Biosensors for Water Toxicity Assessment Sensors Vibrio fischeri toxicity flow injection |
| title | Development of a Fully Automated Flow Injection Analyzer Implementing Bioluminescent Biosensors for Water Toxicity Assessment |
| title_full | Development of a Fully Automated Flow Injection Analyzer Implementing Bioluminescent Biosensors for Water Toxicity Assessment |
| title_fullStr | Development of a Fully Automated Flow Injection Analyzer Implementing Bioluminescent Biosensors for Water Toxicity Assessment |
| title_full_unstemmed | Development of a Fully Automated Flow Injection Analyzer Implementing Bioluminescent Biosensors for Water Toxicity Assessment |
| title_short | Development of a Fully Automated Flow Injection Analyzer Implementing Bioluminescent Biosensors for Water Toxicity Assessment |
| title_sort | development of a fully automated flow injection analyzer implementing bioluminescent biosensors for water toxicity assessment |
| topic | Vibrio fischeri toxicity flow injection |
| url | http://www.mdpi.com/1424-8220/10/8/7089/ |
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