Metabolic and Proteomic Analysis of <i>Chlorella sorokiniana</i>, <i>Chloroidium saccharofilum</i>, and <i>Chlorella vulgaris</i> Cells Cultured in Autotrophic, Photoheterotrophic, and Mixotrophic Cultivation Modes
<i>Chlorella</i> is one of the most well-known microalgal genera, currently comprising approximately a hundred species of single-celled green algae according to the AlgaeBase. Strains of the genus <i>Chlorella</i> have the ability to metabolize both inorganic and organic carb...
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| Format: | Article |
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MDPI AG
2022-07-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/15/4817 |
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| author | Agata Piasecka Andrea Baier |
| author_facet | Agata Piasecka Andrea Baier |
| author_sort | Agata Piasecka |
| collection | DOAJ |
| description | <i>Chlorella</i> is one of the most well-known microalgal genera, currently comprising approximately a hundred species of single-celled green algae according to the AlgaeBase. Strains of the genus <i>Chlorella</i> have the ability to metabolize both inorganic and organic carbon sources in various trophic modes and synthesize valuable metabolites that are widely used in many industries. The aim of this work was to investigate the impact of three trophic modes on the growth parameters, productivities of individual cell components, and biochemical composition of <i>Chlorella sorokiniana, Chloroidium saccharofilum</i><i>,</i> and <i>Chlorella vulgaris</i> cells with special consideration of protein profiles detected by SDS-PAGE gel electrophoresis and two-dimensional gel electrophoresis with MALDI-TOF/TOF MS. Mixotrophic conditions with the use of an agro-industrial by-product stimulated the growth of all <i>Chlorella</i> species, which was confirmed by the highest specific growth rates and the shortest biomass doubling times. The mixotrophic cultivation of all <i>Chlorella</i> species yielded a high amount of protein-rich biomass with reduced contents of chlorophyll a, chlorophyll b, carotenoids, and carbohydrates. Additionally, this work provides the first information about the proteome of <i>Chloroidium saccharofilum</i>, <i>Chlorella sorokiniana</i>, and <i>Chlorella vulgaris</i> cells cultured in molasses supplementation conditions. The proteomic analysis of the three <i>Chlorella</i> species growing photoheterotrophically and mixotrophically showed increased accumulation of proteins involved in the cell energy metabolism and carbon uptake, photosynthesis process, and protein synthesis, as well as proteins involved in intracellular movements and chaperone proteins. |
| first_indexed | 2024-03-09T10:06:25Z |
| format | Article |
| id | doaj.art-4a603f42948046b8a7c2dc8c6533176f |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T10:06:25Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-4a603f42948046b8a7c2dc8c6533176f2023-12-01T23:03:09ZengMDPI AGMolecules1420-30492022-07-012715481710.3390/molecules27154817Metabolic and Proteomic Analysis of <i>Chlorella sorokiniana</i>, <i>Chloroidium saccharofilum</i>, and <i>Chlorella vulgaris</i> Cells Cultured in Autotrophic, Photoheterotrophic, and Mixotrophic Cultivation ModesAgata Piasecka0Andrea Baier1Department of Physical Properties of Plant Materials, Institute of Agrophysics, Polish Academy of Sciences, Doswiadczalna 4, 20-290 Lublin, PolandDepartment of Animal Physiology and Toxicology, The John Paul II Catholic University of Lublin, Konstantynow 1 I, 20-708 Lublin, Poland<i>Chlorella</i> is one of the most well-known microalgal genera, currently comprising approximately a hundred species of single-celled green algae according to the AlgaeBase. Strains of the genus <i>Chlorella</i> have the ability to metabolize both inorganic and organic carbon sources in various trophic modes and synthesize valuable metabolites that are widely used in many industries. The aim of this work was to investigate the impact of three trophic modes on the growth parameters, productivities of individual cell components, and biochemical composition of <i>Chlorella sorokiniana, Chloroidium saccharofilum</i><i>,</i> and <i>Chlorella vulgaris</i> cells with special consideration of protein profiles detected by SDS-PAGE gel electrophoresis and two-dimensional gel electrophoresis with MALDI-TOF/TOF MS. Mixotrophic conditions with the use of an agro-industrial by-product stimulated the growth of all <i>Chlorella</i> species, which was confirmed by the highest specific growth rates and the shortest biomass doubling times. The mixotrophic cultivation of all <i>Chlorella</i> species yielded a high amount of protein-rich biomass with reduced contents of chlorophyll a, chlorophyll b, carotenoids, and carbohydrates. Additionally, this work provides the first information about the proteome of <i>Chloroidium saccharofilum</i>, <i>Chlorella sorokiniana</i>, and <i>Chlorella vulgaris</i> cells cultured in molasses supplementation conditions. The proteomic analysis of the three <i>Chlorella</i> species growing photoheterotrophically and mixotrophically showed increased accumulation of proteins involved in the cell energy metabolism and carbon uptake, photosynthesis process, and protein synthesis, as well as proteins involved in intracellular movements and chaperone proteins.https://www.mdpi.com/1420-3049/27/15/4817proteinsmicroalgae<i>Chlorella</i>proteomicsgel electrophoresismixotrophy |
| spellingShingle | Agata Piasecka Andrea Baier Metabolic and Proteomic Analysis of <i>Chlorella sorokiniana</i>, <i>Chloroidium saccharofilum</i>, and <i>Chlorella vulgaris</i> Cells Cultured in Autotrophic, Photoheterotrophic, and Mixotrophic Cultivation Modes Molecules proteins microalgae <i>Chlorella</i> proteomics gel electrophoresis mixotrophy |
| title | Metabolic and Proteomic Analysis of <i>Chlorella sorokiniana</i>, <i>Chloroidium saccharofilum</i>, and <i>Chlorella vulgaris</i> Cells Cultured in Autotrophic, Photoheterotrophic, and Mixotrophic Cultivation Modes |
| title_full | Metabolic and Proteomic Analysis of <i>Chlorella sorokiniana</i>, <i>Chloroidium saccharofilum</i>, and <i>Chlorella vulgaris</i> Cells Cultured in Autotrophic, Photoheterotrophic, and Mixotrophic Cultivation Modes |
| title_fullStr | Metabolic and Proteomic Analysis of <i>Chlorella sorokiniana</i>, <i>Chloroidium saccharofilum</i>, and <i>Chlorella vulgaris</i> Cells Cultured in Autotrophic, Photoheterotrophic, and Mixotrophic Cultivation Modes |
| title_full_unstemmed | Metabolic and Proteomic Analysis of <i>Chlorella sorokiniana</i>, <i>Chloroidium saccharofilum</i>, and <i>Chlorella vulgaris</i> Cells Cultured in Autotrophic, Photoheterotrophic, and Mixotrophic Cultivation Modes |
| title_short | Metabolic and Proteomic Analysis of <i>Chlorella sorokiniana</i>, <i>Chloroidium saccharofilum</i>, and <i>Chlorella vulgaris</i> Cells Cultured in Autotrophic, Photoheterotrophic, and Mixotrophic Cultivation Modes |
| title_sort | metabolic and proteomic analysis of i chlorella sorokiniana i i chloroidium saccharofilum i and i chlorella vulgaris i cells cultured in autotrophic photoheterotrophic and mixotrophic cultivation modes |
| topic | proteins microalgae <i>Chlorella</i> proteomics gel electrophoresis mixotrophy |
| url | https://www.mdpi.com/1420-3049/27/15/4817 |
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