Biochemical characterization of pectinase activity from the digestive midgut fluid of larvae and adult of the Colorado potato beetle, Leptinotarsa decemlineata (Col: Chrysomelidae)
The study of pectinase enzyme in potato leaf beetle that is the most important pest of potatoes consider as an effective way to develop control methods because this enzyme is important in degrading plant cell wall. Pectinase enzyme was studied in midgut of Colorado potato beetle (CPB). This enzyme w...
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| Format: | Article |
| Language: | English |
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International Academy of Ecology and Environmental Sciences
2015-03-01
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| Series: | Arthropods |
| Subjects: | |
| Online Access: | http://www.iaees.org/publications/journals/arthropods/articles/2015-4(1)/biochemical-characterization-of-pectinase-activity.pdf |
| _version_ | 1828849070839431168 |
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| author | Razieh Karimi Majid Kazzazi Mohammad Vatanparast |
| author_facet | Razieh Karimi Majid Kazzazi Mohammad Vatanparast |
| author_sort | Razieh Karimi |
| collection | DOAJ |
| description | The study of pectinase enzyme in potato leaf beetle that is the most important pest of potatoes consider as an effective way to develop control methods because this enzyme is important in degrading plant cell wall. Pectinase enzyme was studied in midgut of Colorado potato beetle (CPB). This enzyme was extracted from the midgut of larvae and adult of CPB and then their important features were examined by specific substrate, pectin 1 percent. The optimum enzyme activity in 4th instar larvae and adult occurred at pH 5-6 range. Effect of temperature on enzyme activity were examined, the results suggest that the pectinase in midgut of 4th instar larvae and adult shows maximum activity at temperature 40 Celsius degree and 35 Celsius degree, respectively. Zymogram analysis showed presence of two activity bands for pectinase enzyme. The effect of various chemical compounds on the activity of enzyme showed that SDS, Urea and Tris reduced the enzyme activity. NaCl and CaCl2 increased this enzyme activity in 4th instar larvae and adult of CPB. This is first report of pectinase activity in L. decemlineata. |
| first_indexed | 2024-12-12T22:43:18Z |
| format | Article |
| id | doaj.art-4a6040a9be684529b5f13a4b6454e9a7 |
| institution | Directory Open Access Journal |
| issn | 2224-4255 2224-4255 |
| language | English |
| last_indexed | 2024-12-12T22:43:18Z |
| publishDate | 2015-03-01 |
| publisher | International Academy of Ecology and Environmental Sciences |
| record_format | Article |
| series | Arthropods |
| spelling | doaj.art-4a6040a9be684529b5f13a4b6454e9a72022-12-22T00:09:16ZengInternational Academy of Ecology and Environmental SciencesArthropods2224-42552224-42552015-03-01412231Biochemical characterization of pectinase activity from the digestive midgut fluid of larvae and adult of the Colorado potato beetle, Leptinotarsa decemlineata (Col: Chrysomelidae)Razieh Karimi0Majid Kazzazi1Mohammad Vatanparast2Department of Plant Protection, College of Agriculture, Bu Ali-Sina University, Hamedan, IranDepartment of Plant Protection, College of Agriculture, Bu Ali-Sina University, Hamedan, IranDepartment of Plant Protection, College of Agriculture, Bu Ali-Sina University, Hamedan, IranThe study of pectinase enzyme in potato leaf beetle that is the most important pest of potatoes consider as an effective way to develop control methods because this enzyme is important in degrading plant cell wall. Pectinase enzyme was studied in midgut of Colorado potato beetle (CPB). This enzyme was extracted from the midgut of larvae and adult of CPB and then their important features were examined by specific substrate, pectin 1 percent. The optimum enzyme activity in 4th instar larvae and adult occurred at pH 5-6 range. Effect of temperature on enzyme activity were examined, the results suggest that the pectinase in midgut of 4th instar larvae and adult shows maximum activity at temperature 40 Celsius degree and 35 Celsius degree, respectively. Zymogram analysis showed presence of two activity bands for pectinase enzyme. The effect of various chemical compounds on the activity of enzyme showed that SDS, Urea and Tris reduced the enzyme activity. NaCl and CaCl2 increased this enzyme activity in 4th instar larvae and adult of CPB. This is first report of pectinase activity in L. decemlineata.http://www.iaees.org/publications/journals/arthropods/articles/2015-4(1)/biochemical-characterization-of-pectinase-activity.pdfactivityColorado potato beetlemidgutpectinaseZymogram |
| spellingShingle | Razieh Karimi Majid Kazzazi Mohammad Vatanparast Biochemical characterization of pectinase activity from the digestive midgut fluid of larvae and adult of the Colorado potato beetle, Leptinotarsa decemlineata (Col: Chrysomelidae) Arthropods activity Colorado potato beetle midgut pectinase Zymogram |
| title | Biochemical characterization of pectinase activity from the digestive midgut fluid of larvae and adult of the Colorado potato beetle, Leptinotarsa decemlineata (Col: Chrysomelidae) |
| title_full | Biochemical characterization of pectinase activity from the digestive midgut fluid of larvae and adult of the Colorado potato beetle, Leptinotarsa decemlineata (Col: Chrysomelidae) |
| title_fullStr | Biochemical characterization of pectinase activity from the digestive midgut fluid of larvae and adult of the Colorado potato beetle, Leptinotarsa decemlineata (Col: Chrysomelidae) |
| title_full_unstemmed | Biochemical characterization of pectinase activity from the digestive midgut fluid of larvae and adult of the Colorado potato beetle, Leptinotarsa decemlineata (Col: Chrysomelidae) |
| title_short | Biochemical characterization of pectinase activity from the digestive midgut fluid of larvae and adult of the Colorado potato beetle, Leptinotarsa decemlineata (Col: Chrysomelidae) |
| title_sort | biochemical characterization of pectinase activity from the digestive midgut fluid of larvae and adult of the colorado potato beetle leptinotarsa decemlineata col chrysomelidae |
| topic | activity Colorado potato beetle midgut pectinase Zymogram |
| url | http://www.iaees.org/publications/journals/arthropods/articles/2015-4(1)/biochemical-characterization-of-pectinase-activity.pdf |
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