Long pentraxin 3 and vitamin D receptor mRNA expression pattern of cumulus granulosa cells isolated from PCOS oocytes at different stages of nuclear maturation
Abstract Background A fine-tuned pro-inflammatory and anti-inflammatory balance in the follicular unit is essential for cumulus expansion and successful ovulation. While the long pentraxin 3 (PTX3) gene is required for the expansion of cumulus cells (CCs), ovulation, resumption of meiosis and fertil...
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| Format: | Article |
| Language: | English |
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BMC
2024-01-01
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| Series: | Reproductive Biology and Endocrinology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12958-023-01176-5 |
| _version_ | 1797362769851318272 |
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| author | Aynur Ersahin Onder Celik Nur D. Gungor Nilufer Celik Sureyya Melil Meltem Yardim Semih Dalkilic Cenk Ersahin Ece Dogukargin Sudenaz Celik Ramazan F. Akkoc |
| author_facet | Aynur Ersahin Onder Celik Nur D. Gungor Nilufer Celik Sureyya Melil Meltem Yardim Semih Dalkilic Cenk Ersahin Ece Dogukargin Sudenaz Celik Ramazan F. Akkoc |
| author_sort | Aynur Ersahin |
| collection | DOAJ |
| description | Abstract Background A fine-tuned pro-inflammatory and anti-inflammatory balance in the follicular unit is essential for cumulus expansion and successful ovulation. While the long pentraxin 3 (PTX3) gene is required for the expansion of cumulus cells (CCs), ovulation, resumption of meiosis and fertilization, the vitamin D receptor gene (VDR-X2) is required for intra-follicle redox balance. This study was planned to determine the expression pattern of VDR-X2 and PTX3 mRNA in CCs isolated from germinal vesicle (GV), metaphase I (MI), and metaphase II (MII) oocytes of PCOS patients with ovulatory dysfunction. Methods The relative expression of CC-PTX3 and CC-VDR-X2 mRNA were evaluated using qRT-PCR in a total of 79 CC samples collected from individual cumulus-oocyte complex of 40 infertile patients (20 PCOS and 20 non-PCOS normal responders) who underwent ovarian stimulation with the GnRH antagonist protocol. Results Relative PTX3 mRNA expressions of CCMI-control and CCMII-control showed 3- and 9-fold significant upregulation compared to CCGV-control, respectively. The relative PTX3 mRNA expression of CCMII-control increased approximately three fold compared to CCMI-control. Compared to CCGV-pcos, a 3-fold increase was noted in the relative PTX3 mRNA expression of CCMI-pcos and an approximately 4-fold increase in the PTX3 mRNA expression of CCMII-pcos. Relative PTX3 mRNA expression values of CCMII-pcos and CCMI-pcos were similar. A 6-fold upregulation of relative PTX3 mRNA and a 4-fold upregulation of VDR-X2 mRNA were detected in CCMII-control compared to CCMII-pcos. CC-VDR-X2 expression patterns of the PCOS and control groups overlapped with the CC-PTX3 pattern. Fertilization rates of the PCOS group exhibiting failed transcript expression were similar to normal responders. Conclusion The fact that relative CC-PTX3 and CC-VDR mRNA expression does not increase during the transition from MI to MII stage in PCOS as in normal responders suggests that PTX3 and VDR expression may be defective in cumulus cells of PCOS patients with ovulatory dysfunction. |
| first_indexed | 2024-03-08T16:11:16Z |
| format | Article |
| id | doaj.art-4a608c38ef2d4877b51790d193326026 |
| institution | Directory Open Access Journal |
| issn | 1477-7827 |
| language | English |
| last_indexed | 2024-03-08T16:11:16Z |
| publishDate | 2024-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Reproductive Biology and Endocrinology |
| spelling | doaj.art-4a608c38ef2d4877b51790d1933260262024-01-07T12:54:30ZengBMCReproductive Biology and Endocrinology1477-78272024-01-0122111010.1186/s12958-023-01176-5Long pentraxin 3 and vitamin D receptor mRNA expression pattern of cumulus granulosa cells isolated from PCOS oocytes at different stages of nuclear maturationAynur Ersahin0Onder Celik1Nur D. Gungor2Nilufer Celik3Sureyya Melil4Meltem Yardim5Semih Dalkilic6Cenk Ersahin7Ece Dogukargin8Sudenaz Celik9Ramazan F. Akkoc10Department of Obstetrics and Gynecology, Bahcesehir University Goztepe Medicalpark HospitalDepartment of Obstetrics and Gynecology, Private ClinicDepartment of Obstetrics and Gynecology, Bahcesehir University Goztepe Medicalpark HospitalDepartment of Medical Biochemistry, Behcet Uz Children’s HospitalGöztepe Medicalpark Hospital IVF-UnitDepartment of Medical Biochemistry, Yerkoy State HospitalFaculty of Science, Department of Biology, Molecular Biology and Genetics Program, Firat UniversityBahcesehir University School of MedicineBahcesehir University School of MedicineMedical Faculty, Sofia University “St. Kliment Ohridski”Department of Anatomy, School of Medicine, Firat UniversityAbstract Background A fine-tuned pro-inflammatory and anti-inflammatory balance in the follicular unit is essential for cumulus expansion and successful ovulation. While the long pentraxin 3 (PTX3) gene is required for the expansion of cumulus cells (CCs), ovulation, resumption of meiosis and fertilization, the vitamin D receptor gene (VDR-X2) is required for intra-follicle redox balance. This study was planned to determine the expression pattern of VDR-X2 and PTX3 mRNA in CCs isolated from germinal vesicle (GV), metaphase I (MI), and metaphase II (MII) oocytes of PCOS patients with ovulatory dysfunction. Methods The relative expression of CC-PTX3 and CC-VDR-X2 mRNA were evaluated using qRT-PCR in a total of 79 CC samples collected from individual cumulus-oocyte complex of 40 infertile patients (20 PCOS and 20 non-PCOS normal responders) who underwent ovarian stimulation with the GnRH antagonist protocol. Results Relative PTX3 mRNA expressions of CCMI-control and CCMII-control showed 3- and 9-fold significant upregulation compared to CCGV-control, respectively. The relative PTX3 mRNA expression of CCMII-control increased approximately three fold compared to CCMI-control. Compared to CCGV-pcos, a 3-fold increase was noted in the relative PTX3 mRNA expression of CCMI-pcos and an approximately 4-fold increase in the PTX3 mRNA expression of CCMII-pcos. Relative PTX3 mRNA expression values of CCMII-pcos and CCMI-pcos were similar. A 6-fold upregulation of relative PTX3 mRNA and a 4-fold upregulation of VDR-X2 mRNA were detected in CCMII-control compared to CCMII-pcos. CC-VDR-X2 expression patterns of the PCOS and control groups overlapped with the CC-PTX3 pattern. Fertilization rates of the PCOS group exhibiting failed transcript expression were similar to normal responders. Conclusion The fact that relative CC-PTX3 and CC-VDR mRNA expression does not increase during the transition from MI to MII stage in PCOS as in normal responders suggests that PTX3 and VDR expression may be defective in cumulus cells of PCOS patients with ovulatory dysfunction.https://doi.org/10.1186/s12958-023-01176-5Polycystic ovary syndromeCumulus expansionVDR, PTX3, fertilization |
| spellingShingle | Aynur Ersahin Onder Celik Nur D. Gungor Nilufer Celik Sureyya Melil Meltem Yardim Semih Dalkilic Cenk Ersahin Ece Dogukargin Sudenaz Celik Ramazan F. Akkoc Long pentraxin 3 and vitamin D receptor mRNA expression pattern of cumulus granulosa cells isolated from PCOS oocytes at different stages of nuclear maturation Reproductive Biology and Endocrinology Polycystic ovary syndrome Cumulus expansion VDR, PTX3, fertilization |
| title | Long pentraxin 3 and vitamin D receptor mRNA expression pattern of cumulus granulosa cells isolated from PCOS oocytes at different stages of nuclear maturation |
| title_full | Long pentraxin 3 and vitamin D receptor mRNA expression pattern of cumulus granulosa cells isolated from PCOS oocytes at different stages of nuclear maturation |
| title_fullStr | Long pentraxin 3 and vitamin D receptor mRNA expression pattern of cumulus granulosa cells isolated from PCOS oocytes at different stages of nuclear maturation |
| title_full_unstemmed | Long pentraxin 3 and vitamin D receptor mRNA expression pattern of cumulus granulosa cells isolated from PCOS oocytes at different stages of nuclear maturation |
| title_short | Long pentraxin 3 and vitamin D receptor mRNA expression pattern of cumulus granulosa cells isolated from PCOS oocytes at different stages of nuclear maturation |
| title_sort | long pentraxin 3 and vitamin d receptor mrna expression pattern of cumulus granulosa cells isolated from pcos oocytes at different stages of nuclear maturation |
| topic | Polycystic ovary syndrome Cumulus expansion VDR, PTX3, fertilization |
| url | https://doi.org/10.1186/s12958-023-01176-5 |
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