Inhibitory effect of Camellia sinensis, Ilex paraguariensis and Ardisia compressa tea extracts on the proliferation of human head and neck squamous carcinoma cells
In vitro cell proliferation, cell cycle arrest and induction of apoptosis were investigated, using three human head and neck squamous cell carcinoma (HNSCC) cell lines (OSCC-3, SCC-61, and SQ-20B). Aqueous extracts of Camellia sinensis, Ilex paraguariensis, and Ardisia compressa were tested and (−)...
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| Format: | Article |
| Language: | English |
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Elsevier
2016-01-01
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| Series: | Toxicology Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214750016300130 |
| _version_ | 1818381323103895552 |
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| author | Marco Vinicio Ramirez-Mares Hideka Kobayashi Elvira Gonzalez de Mejia |
| author_facet | Marco Vinicio Ramirez-Mares Hideka Kobayashi Elvira Gonzalez de Mejia |
| author_sort | Marco Vinicio Ramirez-Mares |
| collection | DOAJ |
| description | In vitro cell proliferation, cell cycle arrest and induction of apoptosis were investigated, using three human head and neck squamous cell carcinoma (HNSCC) cell lines (OSCC-3, SCC-61, and SQ-20B). Aqueous extracts of Camellia sinensis, Ilex paraguariensis, and Ardisia compressa were tested and (−) epigallocatechin-3-gallate (EGCG) was used for comparison. For EGCG the IC50 values were between 80 and 166 μM and for the extracts among 75 and 505 μM eq. (+) catechin, with C. sinensis demonstrating dominant cytotoxicity. There was not a correlation between antioxidant capacity and cytotoxicity. Flow cytometry analysis revealed similarities in response for EGCG and C. sinensis. The A. compressa extract altered DNA distribution (P < 0.05) and was the most effective in induction of apoptosis via caspases (P < 0.05). Not all HNSCC cells tested responded to the same preventive agents. The fact that A. compressa inhibits HNSCC cell proliferation makes this aqueous extract a potential source of chemopreventive agents. Keywords: Camellia sinensis, Ardisia compressa, Ilex paraguariensis, Polyphenols, Chemoprevention, Head and neck squamous carcinoma cells |
| first_indexed | 2024-12-14T02:32:45Z |
| format | Article |
| id | doaj.art-4a6228029fd54d1a91f1f737fc2c5f23 |
| institution | Directory Open Access Journal |
| issn | 2214-7500 |
| language | English |
| last_indexed | 2024-12-14T02:32:45Z |
| publishDate | 2016-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Toxicology Reports |
| spelling | doaj.art-4a6228029fd54d1a91f1f737fc2c5f232022-12-21T23:20:12ZengElsevierToxicology Reports2214-75002016-01-013269278Inhibitory effect of Camellia sinensis, Ilex paraguariensis and Ardisia compressa tea extracts on the proliferation of human head and neck squamous carcinoma cellsMarco Vinicio Ramirez-Mares0Hideka Kobayashi1Elvira Gonzalez de Mejia2Instituto de Recursos, Universidad del Mar, Ciudad Universitaria, Puerto Ángel, CP 70902 Oaxaca, México; Corresponding author. Fax: +52 958 5843078Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, 228 ERML, MC-051, 1201 W. Gregory Drive, Urbana, IL 61801,USADepartment of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, 228 ERML, MC-051, 1201 W. Gregory Drive, Urbana, IL 61801,USAIn vitro cell proliferation, cell cycle arrest and induction of apoptosis were investigated, using three human head and neck squamous cell carcinoma (HNSCC) cell lines (OSCC-3, SCC-61, and SQ-20B). Aqueous extracts of Camellia sinensis, Ilex paraguariensis, and Ardisia compressa were tested and (−) epigallocatechin-3-gallate (EGCG) was used for comparison. For EGCG the IC50 values were between 80 and 166 μM and for the extracts among 75 and 505 μM eq. (+) catechin, with C. sinensis demonstrating dominant cytotoxicity. There was not a correlation between antioxidant capacity and cytotoxicity. Flow cytometry analysis revealed similarities in response for EGCG and C. sinensis. The A. compressa extract altered DNA distribution (P < 0.05) and was the most effective in induction of apoptosis via caspases (P < 0.05). Not all HNSCC cells tested responded to the same preventive agents. The fact that A. compressa inhibits HNSCC cell proliferation makes this aqueous extract a potential source of chemopreventive agents. Keywords: Camellia sinensis, Ardisia compressa, Ilex paraguariensis, Polyphenols, Chemoprevention, Head and neck squamous carcinoma cellshttp://www.sciencedirect.com/science/article/pii/S2214750016300130 |
| spellingShingle | Marco Vinicio Ramirez-Mares Hideka Kobayashi Elvira Gonzalez de Mejia Inhibitory effect of Camellia sinensis, Ilex paraguariensis and Ardisia compressa tea extracts on the proliferation of human head and neck squamous carcinoma cells Toxicology Reports |
| title | Inhibitory effect of Camellia sinensis, Ilex paraguariensis and Ardisia compressa tea extracts on the proliferation of human head and neck squamous carcinoma cells |
| title_full | Inhibitory effect of Camellia sinensis, Ilex paraguariensis and Ardisia compressa tea extracts on the proliferation of human head and neck squamous carcinoma cells |
| title_fullStr | Inhibitory effect of Camellia sinensis, Ilex paraguariensis and Ardisia compressa tea extracts on the proliferation of human head and neck squamous carcinoma cells |
| title_full_unstemmed | Inhibitory effect of Camellia sinensis, Ilex paraguariensis and Ardisia compressa tea extracts on the proliferation of human head and neck squamous carcinoma cells |
| title_short | Inhibitory effect of Camellia sinensis, Ilex paraguariensis and Ardisia compressa tea extracts on the proliferation of human head and neck squamous carcinoma cells |
| title_sort | inhibitory effect of camellia sinensis ilex paraguariensis and ardisia compressa tea extracts on the proliferation of human head and neck squamous carcinoma cells |
| url | http://www.sciencedirect.com/science/article/pii/S2214750016300130 |
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