Development and Evaluation of a Set of Spike and Receptor Binding Domain-Based Enzyme-Linked Immunosorbent Assays for SARS-CoV-2 Serological Testing

Development and Evaluation of a Set of Spike and Receptor Binding Domain-Based Enzyme-Linked Immunosorbent Assays for SARS-CoV-2 Serological Testing

The implementation and validation of anti-SARS-CoV-2 IgG serological assays are reported in this paper. S1 and RBD proteins were used to coat ELISA plates, and several secondary antibodies served as reporters. The assays were initially validated with 50 RT-PCR positive COVID-19 sera, which showed hi...

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Bibliographic Details
Main Authors: Rosa Camacho-Sandoval, Alejandro Nieto-Patlán, Gregorio Carballo-Uicab, Alejandra Montes-Luna, María C. Jiménez-Martínez, Luis Vallejo-Castillo, Edith González-González, Hugo Iván Arrieta-Oliva, Keyla Gómez-Castellano, Omar U. Guzmán-Bringas, María Pilar Cruz-Domínguez, Gabriela Medina, Laura A. Montiel-Cervantes, Maricela Gordillo-Marín, Roberto Vázquez-Campuzano, Belem Torres-Longoria, Irma López-Martínez, Sonia M. Pérez-Tapia, Juan Carlos Almagro
Format: Article
Language:English
Published: MDPI AG 2021-08-01
Series:Diagnostics
Subjects:
COVID-19
IgG isotypes
serological diagnostics
seroconversion
UDITEST-V2G<sup>®</sup>
Online Access:https://www.mdpi.com/2075-4418/11/8/1506

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