Effects of timolol treatment on pancreatic antioxidant enzymes in streptozotocin-induced diabetic rats: An experimental and computational study
Background: The study aimed to investigate whether timolol-treatment has a beneficial effect on pentose phosphate pathway enzyme activities such as glucose-6-phosphate dehydrogenase (G6PD), 6-phosphogluconate dehydrogenase (6PGDH) enzyme activities and cAMP level in streptozotocin-induced diabetic r...
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| Format: | Article |
| Language: | English |
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Society of Medical Biochemists of Serbia, Belgrade
2019-01-01
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| Series: | Journal of Medical Biochemistry |
| Subjects: | |
| Online Access: | https://scindeks-clanci.ceon.rs/data/pdf/1452-8258/2019/1452-82581903306N.pdf |
| _version_ | 1811286863311273984 |
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| author | Nuray Ulusu Nuriye Gok Muslum Erman Burak Turan Belma |
| author_facet | Nuray Ulusu Nuriye Gok Muslum Erman Burak Turan Belma |
| author_sort | Nuray Ulusu Nuriye |
| collection | DOAJ |
| description | Background: The study aimed to investigate whether timolol-treatment has a beneficial effect on pentose phosphate pathway enzyme activities such as glucose-6-phosphate dehydrogenase (G6PD), 6-phosphogluconate dehydrogenase (6PGDH) enzyme activities and cAMP level in streptozotocin-induced diabetic rats in pancreatic tissues Methods: Diabetes was induced by streptozotocin (STZ) in 3-month old male Wistar rats. The diabetic rats were treated with timolol (5 mg/kg body weight, for 12 weeks) while the control group received saline. Enzyme activities were determined in pancreas tissue. To support our results, we performed in silico calculations, using Protein Data Bank structures. Results: Timolol treatment of STZ-induced diabetic rats had no noteworthy effect on high blood-glucose levels. However, this treatment induced activities of G6PD and 6PGDH in diabetic rats. Timolol treatment significantly increased cAMP level in diabetic pancreatic tissue. We found that timolol cannot bind strongly to either G6PD or 6PGD, but there is a relatively higher binding affinity to adenylyl cyclase, responsible for cAMP production, serving as a regulatory signal via specific cAMP-binding proteins. Conclusions: Our data point out that timolol treatment has beneficial effects on the antioxidant defence mechanism enzymes in the pancreas of STZ-induced diabetic rats. |
| first_indexed | 2024-04-13T03:07:21Z |
| format | Article |
| id | doaj.art-4a66d86e6d504af28d447dc9bc710a7a |
| institution | Directory Open Access Journal |
| issn | 1452-8258 1452-8266 |
| language | English |
| last_indexed | 2024-04-13T03:07:21Z |
| publishDate | 2019-01-01 |
| publisher | Society of Medical Biochemists of Serbia, Belgrade |
| record_format | Article |
| series | Journal of Medical Biochemistry |
| spelling | doaj.art-4a66d86e6d504af28d447dc9bc710a7a2022-12-22T03:05:11ZengSociety of Medical Biochemists of Serbia, BelgradeJournal of Medical Biochemistry1452-82581452-82662019-01-013833063161452-82581903306NEffects of timolol treatment on pancreatic antioxidant enzymes in streptozotocin-induced diabetic rats: An experimental and computational studyNuray Ulusu Nuriye0Gok Muslum1Erman Burak2Turan Belma3Koc University, School of Medicine, Department of Medical Biochemistry, Istanbul, TurkeyHacettepe University, Faculty of Medicine, Department of Medical Biochemistry, Ankara, TurkeyKoc University, School of Engineering, Department of Chemical and Biological Engineering, Istanbul, TurkeyAnkara University, Faculty of Medicine, Department of Biophysics, Ankara, TurkeyBackground: The study aimed to investigate whether timolol-treatment has a beneficial effect on pentose phosphate pathway enzyme activities such as glucose-6-phosphate dehydrogenase (G6PD), 6-phosphogluconate dehydrogenase (6PGDH) enzyme activities and cAMP level in streptozotocin-induced diabetic rats in pancreatic tissues Methods: Diabetes was induced by streptozotocin (STZ) in 3-month old male Wistar rats. The diabetic rats were treated with timolol (5 mg/kg body weight, for 12 weeks) while the control group received saline. Enzyme activities were determined in pancreas tissue. To support our results, we performed in silico calculations, using Protein Data Bank structures. Results: Timolol treatment of STZ-induced diabetic rats had no noteworthy effect on high blood-glucose levels. However, this treatment induced activities of G6PD and 6PGDH in diabetic rats. Timolol treatment significantly increased cAMP level in diabetic pancreatic tissue. We found that timolol cannot bind strongly to either G6PD or 6PGD, but there is a relatively higher binding affinity to adenylyl cyclase, responsible for cAMP production, serving as a regulatory signal via specific cAMP-binding proteins. Conclusions: Our data point out that timolol treatment has beneficial effects on the antioxidant defence mechanism enzymes in the pancreas of STZ-induced diabetic rats.https://scindeks-clanci.ceon.rs/data/pdf/1452-8258/2019/1452-82581903306N.pdfglucose-6-phosphate dehydrogenase6-phosphogluconate dehydrogenasecampdiabetestimolol |
| spellingShingle | Nuray Ulusu Nuriye Gok Muslum Erman Burak Turan Belma Effects of timolol treatment on pancreatic antioxidant enzymes in streptozotocin-induced diabetic rats: An experimental and computational study Journal of Medical Biochemistry glucose-6-phosphate dehydrogenase 6-phosphogluconate dehydrogenase camp diabetes timolol |
| title | Effects of timolol treatment on pancreatic antioxidant enzymes in streptozotocin-induced diabetic rats: An experimental and computational study |
| title_full | Effects of timolol treatment on pancreatic antioxidant enzymes in streptozotocin-induced diabetic rats: An experimental and computational study |
| title_fullStr | Effects of timolol treatment on pancreatic antioxidant enzymes in streptozotocin-induced diabetic rats: An experimental and computational study |
| title_full_unstemmed | Effects of timolol treatment on pancreatic antioxidant enzymes in streptozotocin-induced diabetic rats: An experimental and computational study |
| title_short | Effects of timolol treatment on pancreatic antioxidant enzymes in streptozotocin-induced diabetic rats: An experimental and computational study |
| title_sort | effects of timolol treatment on pancreatic antioxidant enzymes in streptozotocin induced diabetic rats an experimental and computational study |
| topic | glucose-6-phosphate dehydrogenase 6-phosphogluconate dehydrogenase camp diabetes timolol |
| url | https://scindeks-clanci.ceon.rs/data/pdf/1452-8258/2019/1452-82581903306N.pdf |
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