Oxygen tension-independent protection against hypoxic cell killing in rat liver by low sodium
<p>The role of Na<sup>+</sup> in hypoxic injury was evaluated by a time-course analysis of damage in isolated livers perfused with N<sub>2</sub>-saturated buffer containing standard (143 mM) or low (25 mM) Na<sup>+</sup> levels. Trypan blue uptake was used t...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
PAGEPress Publications
2017-05-01
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| Series: | European Journal of Histochemistry |
| Subjects: | |
| Online Access: | http://ejh.it/index.php/ejh/article/view/2798 |
| Summary: | <p>The role of Na<sup>+</sup> in hypoxic injury was evaluated by a time-course analysis of damage in isolated livers perfused with N<sub>2</sub>-saturated buffer containing standard (143 mM) or low (25 mM) Na<sup>+</sup> levels. Trypan blue uptake was used to detect non-viable cells. Under hypoxia with standard-Na<sup>+</sup>, trypan blue uptake began at the border between pericentral areas and periportal regions and increased in the latter zone; using a low-Na<sup>+</sup> buffer, no trypan blue zonation occurred but a homogenous distribution of dye was found associated with sinusoidal endothelial cell (SEC) staining. A decrease in hyaluronic acid (HA) uptake, index of SEC damage, was observed using a low-Na<sup>+</sup> buffer. A time dependent injury was confirmed by an increase in LDH and TBARS levels with standard-Na<sup>+</sup> buffer. Using low-Na<sup>+</sup> buffer, SEC susceptibility appears elevated under hypoxia and hepatocytes was protected, in an oxygen independent manner.</p> |
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| ISSN: | 1121-760X 2038-8306 |