Oxygen tension-independent protection against hypoxic cell killing in rat liver by low sodium
<p>The role of Na<sup>+</sup> in hypoxic injury was evaluated by a time-course analysis of damage in isolated livers perfused with N<sub>2</sub>-saturated buffer containing standard (143 mM) or low (25 mM) Na<sup>+</sup> levels. Trypan blue uptake was used t...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
PAGEPress Publications
2017-05-01
|
| Series: | European Journal of Histochemistry |
| Subjects: | |
| Online Access: | http://ejh.it/index.php/ejh/article/view/2798 |
| _version_ | 1818568875535499264 |
|---|---|
| author | Andrea Ferrigno Laura G. Di Pasqua Clarissa Berardo Veronica Siciliano Plinio Richelmi Mariapia Vairetti |
| author_facet | Andrea Ferrigno Laura G. Di Pasqua Clarissa Berardo Veronica Siciliano Plinio Richelmi Mariapia Vairetti |
| author_sort | Andrea Ferrigno |
| collection | DOAJ |
| description | <p>The role of Na<sup>+</sup> in hypoxic injury was evaluated by a time-course analysis of damage in isolated livers perfused with N<sub>2</sub>-saturated buffer containing standard (143 mM) or low (25 mM) Na<sup>+</sup> levels. Trypan blue uptake was used to detect non-viable cells. Under hypoxia with standard-Na<sup>+</sup>, trypan blue uptake began at the border between pericentral areas and periportal regions and increased in the latter zone; using a low-Na<sup>+</sup> buffer, no trypan blue zonation occurred but a homogenous distribution of dye was found associated with sinusoidal endothelial cell (SEC) staining. A decrease in hyaluronic acid (HA) uptake, index of SEC damage, was observed using a low-Na<sup>+</sup> buffer. A time dependent injury was confirmed by an increase in LDH and TBARS levels with standard-Na<sup>+</sup> buffer. Using low-Na<sup>+</sup> buffer, SEC susceptibility appears elevated under hypoxia and hepatocytes was protected, in an oxygen independent manner.</p> |
| first_indexed | 2024-12-14T06:40:48Z |
| format | Article |
| id | doaj.art-4a683db6a17d4232904fd24e2cfac416 |
| institution | Directory Open Access Journal |
| issn | 1121-760X 2038-8306 |
| language | English |
| last_indexed | 2024-12-14T06:40:48Z |
| publishDate | 2017-05-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | European Journal of Histochemistry |
| spelling | doaj.art-4a683db6a17d4232904fd24e2cfac4162022-12-21T23:13:14ZengPAGEPress PublicationsEuropean Journal of Histochemistry1121-760X2038-83062017-05-0161210.4081/ejh.2017.27981555Oxygen tension-independent protection against hypoxic cell killing in rat liver by low sodiumAndrea Ferrigno0Laura G. Di Pasqua1Clarissa Berardo2Veronica Siciliano3Plinio Richelmi4Mariapia Vairetti5University of Pavia, Department of Internal Medicine and TherapeuticsUniversity of Pavia, Department of Internal Medicine and TherapeuticsUniversity of Pavia, Department of Internal Medicine and TherapeuticsUniversity of Pavia, Department of Internal Medicine and TherapeuticsUniversity of Pavia, Department of Internal Medicine and TherapeuticsUniversity of Pavia, Department of Internal Medicine and Therapeutics<p>The role of Na<sup>+</sup> in hypoxic injury was evaluated by a time-course analysis of damage in isolated livers perfused with N<sub>2</sub>-saturated buffer containing standard (143 mM) or low (25 mM) Na<sup>+</sup> levels. Trypan blue uptake was used to detect non-viable cells. Under hypoxia with standard-Na<sup>+</sup>, trypan blue uptake began at the border between pericentral areas and periportal regions and increased in the latter zone; using a low-Na<sup>+</sup> buffer, no trypan blue zonation occurred but a homogenous distribution of dye was found associated with sinusoidal endothelial cell (SEC) staining. A decrease in hyaluronic acid (HA) uptake, index of SEC damage, was observed using a low-Na<sup>+</sup> buffer. A time dependent injury was confirmed by an increase in LDH and TBARS levels with standard-Na<sup>+</sup> buffer. Using low-Na<sup>+</sup> buffer, SEC susceptibility appears elevated under hypoxia and hepatocytes was protected, in an oxygen independent manner.</p>http://ejh.it/index.php/ejh/article/view/2798LiverhypoxiaNa+hepatocytessinusoidal endothelial cells. |
| spellingShingle | Andrea Ferrigno Laura G. Di Pasqua Clarissa Berardo Veronica Siciliano Plinio Richelmi Mariapia Vairetti Oxygen tension-independent protection against hypoxic cell killing in rat liver by low sodium European Journal of Histochemistry Liver hypoxia Na+ hepatocytes sinusoidal endothelial cells. |
| title | Oxygen tension-independent protection against hypoxic cell killing in rat liver by low sodium |
| title_full | Oxygen tension-independent protection against hypoxic cell killing in rat liver by low sodium |
| title_fullStr | Oxygen tension-independent protection against hypoxic cell killing in rat liver by low sodium |
| title_full_unstemmed | Oxygen tension-independent protection against hypoxic cell killing in rat liver by low sodium |
| title_short | Oxygen tension-independent protection against hypoxic cell killing in rat liver by low sodium |
| title_sort | oxygen tension independent protection against hypoxic cell killing in rat liver by low sodium |
| topic | Liver hypoxia Na+ hepatocytes sinusoidal endothelial cells. |
| url | http://ejh.it/index.php/ejh/article/view/2798 |
| work_keys_str_mv | AT andreaferrigno oxygentensionindependentprotectionagainsthypoxiccellkillinginratliverbylowsodium AT lauragdipasqua oxygentensionindependentprotectionagainsthypoxiccellkillinginratliverbylowsodium AT clarissaberardo oxygentensionindependentprotectionagainsthypoxiccellkillinginratliverbylowsodium AT veronicasiciliano oxygentensionindependentprotectionagainsthypoxiccellkillinginratliverbylowsodium AT pliniorichelmi oxygentensionindependentprotectionagainsthypoxiccellkillinginratliverbylowsodium AT mariapiavairetti oxygentensionindependentprotectionagainsthypoxiccellkillinginratliverbylowsodium |