Conversion of <i>Escherichia coli</i> into Mixotrophic CO<sub>2</sub> Assimilation with Malate and Hydrogen Based on Recombinant Expression of 2-Oxoglutarate:Ferredoxin Oxidoreductase Using Adaptive Laboratory Evolution

Conversion of <i>Escherichia coli</i> into Mixotrophic CO<sub>2</sub> Assimilation with Malate and Hydrogen Based on Recombinant Expression of 2-Oxoglutarate:Ferredoxin Oxidoreductase Using Adaptive Laboratory Evolution

We report the mixotrophic growth of <i>Escherichia coli</i> based on recombinant 2-oxoglutarate:ferredoxin oxidoreductase (OGOR) to assimilate CO<sub>2</sub> using malate as an auxiliary carbon source and hydrogen as an energy source. We employ a long-term (~184 days) two-sta...

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Bibliographic Details
Main Authors: Yu-Chen Cheng, Wei-Han Huang, Shou-Chen Lo, Eugene Huang, En-Pei Isabel Chiang, Chieh-Chen Huang, Ya-Tang Yang
Format: Article
Language:English
Published: MDPI AG 2023-01-01
Series:Microorganisms
Subjects:
carbon fixation
reductive tricarboxylic acid cycle
2-oxoglutarate:ferredoxin oxidoreductase
adaptive laboratory evolution
Online Access:https://www.mdpi.com/2076-2607/11/2/253

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https://www.mdpi.com/2076-2607/11/2/253

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