Prognostic Value and Related Regulatory Networks of MRPL15 in Non-Small-Cell Lung Cancer
BackgroundMitochondrial ribosomal protein L15 (MRPL15), a member of mitochondrial ribosomal proteins whose abnormal expression is related to tumorigenesis. However, the prognostic value and regulatory mechanisms of MRPL15 in non-small-cell lung cancer (NSCLC) remain unclear.MethodsGEPIA, ONCOMINE, G...
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| Format: | Article |
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Frontiers Media S.A.
2021-05-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2021.656172/full |
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| author | Yangyang Zeng Yangyang Zeng Yingying Shi Yingying Shi Lu Xu Lu Xu Yulan Zeng Xiao Cui Xiao Cui Yuan Wang Yuan Wang Ningning Yang Ningning Yang Fuxiang Zhou Fuxiang Zhou Yunfeng Zhou Yunfeng Zhou |
| author_facet | Yangyang Zeng Yangyang Zeng Yingying Shi Yingying Shi Lu Xu Lu Xu Yulan Zeng Xiao Cui Xiao Cui Yuan Wang Yuan Wang Ningning Yang Ningning Yang Fuxiang Zhou Fuxiang Zhou Yunfeng Zhou Yunfeng Zhou |
| author_sort | Yangyang Zeng |
| collection | DOAJ |
| description | BackgroundMitochondrial ribosomal protein L15 (MRPL15), a member of mitochondrial ribosomal proteins whose abnormal expression is related to tumorigenesis. However, the prognostic value and regulatory mechanisms of MRPL15 in non-small-cell lung cancer (NSCLC) remain unclear.MethodsGEPIA, ONCOMINE, Gene Expression Omnibus (GEO), UALCAN, Kaplan–Meier plotter, PrognoScan, LinkedOmics and GeneMANIA database were utilized to explore the expression and prognostic value of MRPL15 in NSCLC. Additionally, immune infiltration patterns were evaluated via ESTIMATE algorithm and TISIDB database. Furthermore, the expression and prognostic value of MRPL15 in lung cancer were validated via immunohistochemistry (IHC) assays.ResultsIn NSCLC, multiple cohorts including GEPIA, ONCOMINE and 8 GEO series (GSE8569, GSE101929, GSE33532, GSE27262, GSE21933, GSE19804, GSE19188, GSE18842) described that MRPL15 was up-regulated. Moreover, MRPL15 was notably linked to gender, clinical stage, lymph node status and the TP53 mutation status. And patients with high MRPL15 expression showed poor overall survival (OS), progression-free survival (PFS), disease-free survival (DFS) and relapse-free survival (RFS) in NSCLC. Then, functional network analysis suggested that MRPL15 participated in metabolism-related pathways, DNA replication and cell cycle signaling via pathways involving several kinases, miRNAs and transcription factors. Additionally, it was found that MRPL15 expression was negatively related to immune infiltration, including immune scores, stromal scores and several tumor-infiltrating lymphocytes (TILs). Furthermore, IHC results further confirmed the high MRPL15 expression and its prognostic potential in lung cancer.ConclusionsThese findings demonstrate that high MRPL15 expression indicates poor prognosis in NSCLC and reveal potential regulatory networks as well as the negative relationship with immune infiltration. Thus, MRPL15 may be an attractive predictor and therapeutic strategy for NSCLC. |
| first_indexed | 2024-12-20T06:17:19Z |
| format | Article |
| id | doaj.art-4a6a391b8baa48838d374d156e60d73f |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-12-20T06:17:19Z |
| publishDate | 2021-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-4a6a391b8baa48838d374d156e60d73f2022-12-21T19:50:31ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2021-05-011110.3389/fonc.2021.656172656172Prognostic Value and Related Regulatory Networks of MRPL15 in Non-Small-Cell Lung CancerYangyang Zeng0Yangyang Zeng1Yingying Shi2Yingying Shi3Lu Xu4Lu Xu5Yulan Zeng6Xiao Cui7Xiao Cui8Yuan Wang9Yuan Wang10Ningning Yang11Ningning Yang12Fuxiang Zhou13Fuxiang Zhou14Yunfeng Zhou15Yunfeng Zhou16Hubei Key Laboratory of Tumor Biological Behaviors, Zhongnan Hospital of Wuhan University, Wuhan, ChinaDepartment of Radiation and Medical Oncology, Zhongnan Hospital of Wuhan University, Wuhan, ChinaHubei Key Laboratory of Tumor Biological Behaviors, Zhongnan Hospital of Wuhan University, Wuhan, ChinaDepartment of Radiation and Medical Oncology, Zhongnan Hospital of Wuhan University, Wuhan, ChinaHubei Key Laboratory of Tumor Biological Behaviors, Zhongnan Hospital of Wuhan University, Wuhan, ChinaDepartment of Radiation and Medical Oncology, Zhongnan Hospital of Wuhan University, Wuhan, ChinaCancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaHubei Key Laboratory of Tumor Biological Behaviors, Zhongnan Hospital of Wuhan University, Wuhan, ChinaDepartment of Radiation and Medical Oncology, Zhongnan Hospital of Wuhan University, Wuhan, ChinaHubei Key Laboratory of Tumor Biological Behaviors, Zhongnan Hospital of Wuhan University, Wuhan, ChinaDepartment of Radiation and Medical Oncology, Zhongnan Hospital of Wuhan University, Wuhan, ChinaHubei Key Laboratory of Tumor Biological Behaviors, Zhongnan Hospital of Wuhan University, Wuhan, ChinaDepartment of Radiation and Medical Oncology, Zhongnan Hospital of Wuhan University, Wuhan, ChinaHubei Key Laboratory of Tumor Biological Behaviors, Zhongnan Hospital of Wuhan University, Wuhan, ChinaDepartment of Radiation and Medical Oncology, Zhongnan Hospital of Wuhan University, Wuhan, ChinaHubei Key Laboratory of Tumor Biological Behaviors, Zhongnan Hospital of Wuhan University, Wuhan, ChinaDepartment of Radiation and Medical Oncology, Zhongnan Hospital of Wuhan University, Wuhan, ChinaBackgroundMitochondrial ribosomal protein L15 (MRPL15), a member of mitochondrial ribosomal proteins whose abnormal expression is related to tumorigenesis. However, the prognostic value and regulatory mechanisms of MRPL15 in non-small-cell lung cancer (NSCLC) remain unclear.MethodsGEPIA, ONCOMINE, Gene Expression Omnibus (GEO), UALCAN, Kaplan–Meier plotter, PrognoScan, LinkedOmics and GeneMANIA database were utilized to explore the expression and prognostic value of MRPL15 in NSCLC. Additionally, immune infiltration patterns were evaluated via ESTIMATE algorithm and TISIDB database. Furthermore, the expression and prognostic value of MRPL15 in lung cancer were validated via immunohistochemistry (IHC) assays.ResultsIn NSCLC, multiple cohorts including GEPIA, ONCOMINE and 8 GEO series (GSE8569, GSE101929, GSE33532, GSE27262, GSE21933, GSE19804, GSE19188, GSE18842) described that MRPL15 was up-regulated. Moreover, MRPL15 was notably linked to gender, clinical stage, lymph node status and the TP53 mutation status. And patients with high MRPL15 expression showed poor overall survival (OS), progression-free survival (PFS), disease-free survival (DFS) and relapse-free survival (RFS) in NSCLC. Then, functional network analysis suggested that MRPL15 participated in metabolism-related pathways, DNA replication and cell cycle signaling via pathways involving several kinases, miRNAs and transcription factors. Additionally, it was found that MRPL15 expression was negatively related to immune infiltration, including immune scores, stromal scores and several tumor-infiltrating lymphocytes (TILs). Furthermore, IHC results further confirmed the high MRPL15 expression and its prognostic potential in lung cancer.ConclusionsThese findings demonstrate that high MRPL15 expression indicates poor prognosis in NSCLC and reveal potential regulatory networks as well as the negative relationship with immune infiltration. Thus, MRPL15 may be an attractive predictor and therapeutic strategy for NSCLC.https://www.frontiersin.org/articles/10.3389/fonc.2021.656172/fullMRPL15non-small-cell lung cancerprognostic valuefunctional network analysisimmune infiltration |
| spellingShingle | Yangyang Zeng Yangyang Zeng Yingying Shi Yingying Shi Lu Xu Lu Xu Yulan Zeng Xiao Cui Xiao Cui Yuan Wang Yuan Wang Ningning Yang Ningning Yang Fuxiang Zhou Fuxiang Zhou Yunfeng Zhou Yunfeng Zhou Prognostic Value and Related Regulatory Networks of MRPL15 in Non-Small-Cell Lung Cancer Frontiers in Oncology MRPL15 non-small-cell lung cancer prognostic value functional network analysis immune infiltration |
| title | Prognostic Value and Related Regulatory Networks of MRPL15 in Non-Small-Cell Lung Cancer |
| title_full | Prognostic Value and Related Regulatory Networks of MRPL15 in Non-Small-Cell Lung Cancer |
| title_fullStr | Prognostic Value and Related Regulatory Networks of MRPL15 in Non-Small-Cell Lung Cancer |
| title_full_unstemmed | Prognostic Value and Related Regulatory Networks of MRPL15 in Non-Small-Cell Lung Cancer |
| title_short | Prognostic Value and Related Regulatory Networks of MRPL15 in Non-Small-Cell Lung Cancer |
| title_sort | prognostic value and related regulatory networks of mrpl15 in non small cell lung cancer |
| topic | MRPL15 non-small-cell lung cancer prognostic value functional network analysis immune infiltration |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2021.656172/full |
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