Potential therapeutic applications of infusions and hydroalcoholic extracts of Romanian glutinous sage (Salvia glutinosa L.)
Ethnopharmacological relevance:Salvia glutinosa, also known as the glutinous sage, has been used in Romanian folk medicine in the treatment of inflammation, injuries, and mild infections. However, there is no direct scientific evidence to demonstrate these activities.Aim of the Study: The present re...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2022.975800/full |
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| author | Alexandru Nicolescu Mihai Babotă Maria Ilea Maria Inês Dias Ricardo C. Calhelha Laura Gavrilaș Gabriele Rocchetti Gianina Crișan Andrei Mocan Andrei Mocan Lillian Barros Alina Elena Pârvu |
| author_facet | Alexandru Nicolescu Mihai Babotă Maria Ilea Maria Inês Dias Ricardo C. Calhelha Laura Gavrilaș Gabriele Rocchetti Gianina Crișan Andrei Mocan Andrei Mocan Lillian Barros Alina Elena Pârvu |
| author_sort | Alexandru Nicolescu |
| collection | DOAJ |
| description | Ethnopharmacological relevance:Salvia glutinosa, also known as the glutinous sage, has been used in Romanian folk medicine in the treatment of inflammation, injuries, and mild infections. However, there is no direct scientific evidence to demonstrate these activities.Aim of the Study: The present research was based on evaluating antioxidant, antiproliferative, and α-glucosidase inhibitory activity of S. glutinosa extracts, as well as the in vivo anti-inflammatory activity.Materials and Methods: Infusions and 70% (v:v) ethanol solution extracts of S. glutinosa stems and leaves, collected from two different locations in Romania, were prepared. Ten phenolic compounds were identified and quantified using the LC-DAD-ESI/MSn method, and total phenolic and flavonoid content, as well as in vitro antioxidant (DPPH, ABTS, and FRAP assays), antiproliferative, anti-inflammatory and alpha-glucosidase inhibitory activities were determined. A rat model of induced inflammation with turpentine oil was used for the examination of in vivo effects of the extracts, using diclofenac as an anti-inflammatory control.Results: The highest inhibitory α-glucosidase activity was determined to be IC50 = 0.546 mg/ml for the hydroalcoholic extract made with plant material collected on the road to Sighișoara. The highest cytotoxic activity against HepG2 cell line was determined to be GI50 = 131.68 ± 5.03 μg/ml, for the hydroalcoholic extract made with plant material from Sighișoara. In vivo administration of extract (200 mg lyophilized powder/ml) showed a significant reduction of NO production.Conclusion: Our findings indicate that S. glutinosa extracts exhibit antioxidant, α-glucosidase inhibitory activity, as well as a modest cytotoxic effect on HepG2 cell line. By in vivo administration, the extracts show anti-inflammatory and antioxidant activity, which correlates with the traditional use of the species. The environmental conditions seemed to induce important changes in the chemical composition and the bioactivity of the herbal preparations derived from S. glutinosa. |
| first_indexed | 2024-04-13T18:31:23Z |
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| id | doaj.art-4a6a5df8ec7f4318827504c82d8980c5 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-04-13T18:31:23Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-4a6a5df8ec7f4318827504c82d8980c52022-12-22T02:35:05ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122022-08-011310.3389/fphar.2022.975800975800Potential therapeutic applications of infusions and hydroalcoholic extracts of Romanian glutinous sage (Salvia glutinosa L.)Alexandru Nicolescu0Mihai Babotă1Maria Ilea2Maria Inês Dias3Ricardo C. Calhelha4Laura Gavrilaș5Gabriele Rocchetti6Gianina Crișan7Andrei Mocan8Andrei Mocan9Lillian Barros10Alina Elena Pârvu11Department of Pharmaceutical Botany, “Iuliu Hațieganu” University of Medicine and Pharmacy, Cluj-Napoca, RomaniaDepartment of Pharmaceutical Botany, “Iuliu Hațieganu” University of Medicine and Pharmacy, Cluj-Napoca, RomaniaDepartment of Pathophysiology, Faculty of Medicine, “Iuliu Hațieganu” University of Medicine and Pharmacy, Cluj-Napoca, RomaniaCentro de Investigação de Montanha (CIMO), Instituto Politécnico de Bragança, Bragança, PortugalCentro de Investigação de Montanha (CIMO), Instituto Politécnico de Bragança, Bragança, PortugalDepartment of Bromatology, Hygiene, Nutrition, “Iuliu Hațieganu” University of Medicine and Pharmacy, Cluj-Napoca, RomaniaDepartment of Animal Science, Food and Nutrition, Università Cattolica del Sacro Cuore, Piacenza, ItalyDepartment of Pharmaceutical Botany, “Iuliu Hațieganu” University of Medicine and Pharmacy, Cluj-Napoca, RomaniaDepartment of Pharmaceutical Botany, “Iuliu Hațieganu” University of Medicine and Pharmacy, Cluj-Napoca, RomaniaLaboratory of Chromatography, Institute of Advanced Horticulture Research of Transylvania, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, RomaniaCentro de Investigação de Montanha (CIMO), Instituto Politécnico de Bragança, Bragança, PortugalDepartment of Pathophysiology, Faculty of Medicine, “Iuliu Hațieganu” University of Medicine and Pharmacy, Cluj-Napoca, RomaniaEthnopharmacological relevance:Salvia glutinosa, also known as the glutinous sage, has been used in Romanian folk medicine in the treatment of inflammation, injuries, and mild infections. However, there is no direct scientific evidence to demonstrate these activities.Aim of the Study: The present research was based on evaluating antioxidant, antiproliferative, and α-glucosidase inhibitory activity of S. glutinosa extracts, as well as the in vivo anti-inflammatory activity.Materials and Methods: Infusions and 70% (v:v) ethanol solution extracts of S. glutinosa stems and leaves, collected from two different locations in Romania, were prepared. Ten phenolic compounds were identified and quantified using the LC-DAD-ESI/MSn method, and total phenolic and flavonoid content, as well as in vitro antioxidant (DPPH, ABTS, and FRAP assays), antiproliferative, anti-inflammatory and alpha-glucosidase inhibitory activities were determined. A rat model of induced inflammation with turpentine oil was used for the examination of in vivo effects of the extracts, using diclofenac as an anti-inflammatory control.Results: The highest inhibitory α-glucosidase activity was determined to be IC50 = 0.546 mg/ml for the hydroalcoholic extract made with plant material collected on the road to Sighișoara. The highest cytotoxic activity against HepG2 cell line was determined to be GI50 = 131.68 ± 5.03 μg/ml, for the hydroalcoholic extract made with plant material from Sighișoara. In vivo administration of extract (200 mg lyophilized powder/ml) showed a significant reduction of NO production.Conclusion: Our findings indicate that S. glutinosa extracts exhibit antioxidant, α-glucosidase inhibitory activity, as well as a modest cytotoxic effect on HepG2 cell line. By in vivo administration, the extracts show anti-inflammatory and antioxidant activity, which correlates with the traditional use of the species. The environmental conditions seemed to induce important changes in the chemical composition and the bioactivity of the herbal preparations derived from S. glutinosa.https://www.frontiersin.org/articles/10.3389/fphar.2022.975800/fullSalvia glutinosaanti-inflammatoryROSantioxidantalpha-glucosidasepolyphenols |
| spellingShingle | Alexandru Nicolescu Mihai Babotă Maria Ilea Maria Inês Dias Ricardo C. Calhelha Laura Gavrilaș Gabriele Rocchetti Gianina Crișan Andrei Mocan Andrei Mocan Lillian Barros Alina Elena Pârvu Potential therapeutic applications of infusions and hydroalcoholic extracts of Romanian glutinous sage (Salvia glutinosa L.) Frontiers in Pharmacology Salvia glutinosa anti-inflammatory ROS antioxidant alpha-glucosidase polyphenols |
| title | Potential therapeutic applications of infusions and hydroalcoholic extracts of Romanian glutinous sage (Salvia glutinosa L.) |
| title_full | Potential therapeutic applications of infusions and hydroalcoholic extracts of Romanian glutinous sage (Salvia glutinosa L.) |
| title_fullStr | Potential therapeutic applications of infusions and hydroalcoholic extracts of Romanian glutinous sage (Salvia glutinosa L.) |
| title_full_unstemmed | Potential therapeutic applications of infusions and hydroalcoholic extracts of Romanian glutinous sage (Salvia glutinosa L.) |
| title_short | Potential therapeutic applications of infusions and hydroalcoholic extracts of Romanian glutinous sage (Salvia glutinosa L.) |
| title_sort | potential therapeutic applications of infusions and hydroalcoholic extracts of romanian glutinous sage salvia glutinosa l |
| topic | Salvia glutinosa anti-inflammatory ROS antioxidant alpha-glucosidase polyphenols |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2022.975800/full |
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