Evaluation of Fluorescent Cu<sup>2+</sup> Probes: Instant Sensing, Cell Permeable Recognition and Quantitative Detection
By incorporating a rhodamine spirolactam structure as the recognition site for Cu<sup>2+</sup>, two novel probes were synthesized through a connection of rhodamine 6G acylhydrazine and 5-formyl-6-hydroxyl-4-methylcoumarin/2,4-dihydroxybenzaldehyde. In the recognition process of probes to...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-01-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/2/512 |
| _version_ | 1827599638122725376 |
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| author | Hao He Zhao Cheng Lei Zheng Xuejiao Zhang |
| author_facet | Hao He Zhao Cheng Lei Zheng Xuejiao Zhang |
| author_sort | Hao He |
| collection | DOAJ |
| description | By incorporating a rhodamine spirolactam structure as the recognition site for Cu<sup>2+</sup>, two novel probes were synthesized through a connection of rhodamine 6G acylhydrazine and 5-formyl-6-hydroxyl-4-methylcoumarin/2,4-dihydroxybenzaldehyde. In the recognition process of probes towards Cu<sup>2+</sup>, the spirolactam ring exhibited opening and closing, accompanying an instant and specific change in fluorescence and in color, which could also achieve a naked-eye and semiquantitative recognition of aqueous Cu<sup>2+</sup> besides the fluorescent Cu<sup>2+</sup> detection method. Fluorescent analyses and ECV304 cell imaging further revealed the probes’ good optical stability, instant response, low toxicity, and membrane permeability, which offers future possibilities for the probes’ instant detection and the real-time tracking of Cu<sup>2+</sup> in biological systems. |
| first_indexed | 2024-03-09T04:17:52Z |
| format | Article |
| id | doaj.art-4a6b1a7d4fa64deba2e77dad5abd0c33 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T04:17:52Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-4a6b1a7d4fa64deba2e77dad5abd0c332023-12-03T13:51:06ZengMDPI AGMolecules1420-30492021-01-0126251210.3390/molecules26020512Evaluation of Fluorescent Cu<sup>2+</sup> Probes: Instant Sensing, Cell Permeable Recognition and Quantitative DetectionHao He0Zhao Cheng1Lei Zheng2Xuejiao Zhang3School of Pharmacy, Xi’an Medical University, Xi’an 710021, ChinaSchool of Pharmacy, Xi’an Medical University, Xi’an 710021, ChinaSchool of Pharmacy, Xi’an Medical University, Xi’an 710021, ChinaSchool of Pharmacy, Xi’an Medical University, Xi’an 710021, ChinaBy incorporating a rhodamine spirolactam structure as the recognition site for Cu<sup>2+</sup>, two novel probes were synthesized through a connection of rhodamine 6G acylhydrazine and 5-formyl-6-hydroxyl-4-methylcoumarin/2,4-dihydroxybenzaldehyde. In the recognition process of probes towards Cu<sup>2+</sup>, the spirolactam ring exhibited opening and closing, accompanying an instant and specific change in fluorescence and in color, which could also achieve a naked-eye and semiquantitative recognition of aqueous Cu<sup>2+</sup> besides the fluorescent Cu<sup>2+</sup> detection method. Fluorescent analyses and ECV304 cell imaging further revealed the probes’ good optical stability, instant response, low toxicity, and membrane permeability, which offers future possibilities for the probes’ instant detection and the real-time tracking of Cu<sup>2+</sup> in biological systems.https://www.mdpi.com/1420-3049/26/2/512rhodaminenaked-eye recognitioncopper ionsdynamic bioimagingsemiquantitative test paper |
| spellingShingle | Hao He Zhao Cheng Lei Zheng Xuejiao Zhang Evaluation of Fluorescent Cu<sup>2+</sup> Probes: Instant Sensing, Cell Permeable Recognition and Quantitative Detection Molecules rhodamine naked-eye recognition copper ions dynamic bioimaging semiquantitative test paper |
| title | Evaluation of Fluorescent Cu<sup>2+</sup> Probes: Instant Sensing, Cell Permeable Recognition and Quantitative Detection |
| title_full | Evaluation of Fluorescent Cu<sup>2+</sup> Probes: Instant Sensing, Cell Permeable Recognition and Quantitative Detection |
| title_fullStr | Evaluation of Fluorescent Cu<sup>2+</sup> Probes: Instant Sensing, Cell Permeable Recognition and Quantitative Detection |
| title_full_unstemmed | Evaluation of Fluorescent Cu<sup>2+</sup> Probes: Instant Sensing, Cell Permeable Recognition and Quantitative Detection |
| title_short | Evaluation of Fluorescent Cu<sup>2+</sup> Probes: Instant Sensing, Cell Permeable Recognition and Quantitative Detection |
| title_sort | evaluation of fluorescent cu sup 2 sup probes instant sensing cell permeable recognition and quantitative detection |
| topic | rhodamine naked-eye recognition copper ions dynamic bioimaging semiquantitative test paper |
| url | https://www.mdpi.com/1420-3049/26/2/512 |
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