The novel immunobiotic Clostridium butyricum S-45-5 displays broad-spectrum antiviral activity in vitro and in vivo by inducing immune modulation
Clostridium butyricum is known as a probiotic butyric acid bacterium that can improve the intestinal environment. In this study, we isolated a new strain of C. butyricum from infant feces and evaluated its physiological characteristics and antiviral efficacy by modulating the innate immune responses...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-10-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1242183/full |
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| author | Kiramage Chathuranga Yeseul Shin Md Bashir Uddin Md Bashir Uddin Md Bashir Uddin Jayoung Paek W. A. Gayan Chathuranga Yebin Seong Lu Bai Hongik Kim Jeong Hwan Shin Young-Hyo Chang Jong-Soo Lee |
| author_facet | Kiramage Chathuranga Yeseul Shin Md Bashir Uddin Md Bashir Uddin Md Bashir Uddin Jayoung Paek W. A. Gayan Chathuranga Yebin Seong Lu Bai Hongik Kim Jeong Hwan Shin Young-Hyo Chang Jong-Soo Lee |
| author_sort | Kiramage Chathuranga |
| collection | DOAJ |
| description | Clostridium butyricum is known as a probiotic butyric acid bacterium that can improve the intestinal environment. In this study, we isolated a new strain of C. butyricum from infant feces and evaluated its physiological characteristics and antiviral efficacy by modulating the innate immune responses in vitro and in vivo. The isolated C. butyricum S-45-5 showed typical characteristics of C. butyricum including bile acid resistance, antibacterial ability, and growth promotion of various lactic acid bacteria. As an antiviral effect, C. butyricum S-45-5 markedly reduced the replication of influenza A virus (PR8), Newcastle Disease Virus (NDV), and Herpes Simplex Virus (HSV) in RAW264.7 cells in vitro. This suppression can be explained by the induction of antiviral state in cells by the induction of antiviral, IFN-related genes and secretion of IFNs and pro-inflammatory cytokines. In vivo, oral administration of C. butyricum S-45-5 exhibited prophylactic effects on BALB/c mice against fatal doses of highly pathogenic mouse-adapted influenza A subtypes (H1N1, H3N2, and H9N2). Before challenge with influenza virus, C. butyricum S-45-5-treated BALB/c mice showed increased levels of IFN-β, IFN-γ, IL-6, and IL-12 in serum, the small intestine, and bronchoalveolar lavage fluid (BALF), which correlated with observed prophylactic effects. Interestingly, after challenge with influenza virus, C. butyricum S-45-5-treated BALB/c mice showed reduced levels of pro-inflammatory cytokines and relatively higher levels of anti-inflammatory cytokines at day 7 post-infection. Taken together, these findings suggest that C. butyricum S-45-5 plays an antiviral role in vitro and in vivo by inducing an antiviral state and affects immune modulation to alleviate local and systemic inflammatory responses caused by influenza virus infection. Our study provides the beneficial effects of the new C. butyricum S-45-5 with antiviral effects as a probiotic. |
| first_indexed | 2024-03-11T19:07:09Z |
| format | Article |
| id | doaj.art-4a7a8b8cdaa6437d845f6e4a58d49a6d |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-03-11T19:07:09Z |
| publishDate | 2023-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-4a7a8b8cdaa6437d845f6e4a58d49a6d2023-10-10T04:57:38ZengFrontiers Media S.A.Frontiers in Immunology1664-32242023-10-011410.3389/fimmu.2023.12421831242183The novel immunobiotic Clostridium butyricum S-45-5 displays broad-spectrum antiviral activity in vitro and in vivo by inducing immune modulationKiramage Chathuranga0Yeseul Shin1Md Bashir Uddin2Md Bashir Uddin3Md Bashir Uddin4Jayoung Paek5W. A. Gayan Chathuranga6Yebin Seong7Lu Bai8Hongik Kim9Jeong Hwan Shin10Young-Hyo Chang11Jong-Soo Lee12College of Veterinary Medicine, Chungnam National University, Daejeon, Republic of KoreaAccess to Genetic Resources and Benefit-Sharing (ABS) Research Support Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of KoreaCollege of Veterinary Medicine, Chungnam National University, Daejeon, Republic of KoreaDepartment of Medicine, Sylhet Agricultural University, Sylhet, BangladeshDepartment of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX, United StatesAccess to Genetic Resources and Benefit-Sharing (ABS) Research Support Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of KoreaCollege of Veterinary Medicine, Chungnam National University, Daejeon, Republic of KoreaCollege of Veterinary Medicine, Chungnam National University, Daejeon, Republic of KoreaAccess to Genetic Resources and Benefit-Sharing (ABS) Research Support Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of KoreaResearch and Development Division, Vitabio, Inc., Daejeon, Republic of KoreaDepartment of Laboratory Medicine, Inje University College of Medicine, Busan, Republic of KoreaAccess to Genetic Resources and Benefit-Sharing (ABS) Research Support Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of KoreaCollege of Veterinary Medicine, Chungnam National University, Daejeon, Republic of KoreaClostridium butyricum is known as a probiotic butyric acid bacterium that can improve the intestinal environment. In this study, we isolated a new strain of C. butyricum from infant feces and evaluated its physiological characteristics and antiviral efficacy by modulating the innate immune responses in vitro and in vivo. The isolated C. butyricum S-45-5 showed typical characteristics of C. butyricum including bile acid resistance, antibacterial ability, and growth promotion of various lactic acid bacteria. As an antiviral effect, C. butyricum S-45-5 markedly reduced the replication of influenza A virus (PR8), Newcastle Disease Virus (NDV), and Herpes Simplex Virus (HSV) in RAW264.7 cells in vitro. This suppression can be explained by the induction of antiviral state in cells by the induction of antiviral, IFN-related genes and secretion of IFNs and pro-inflammatory cytokines. In vivo, oral administration of C. butyricum S-45-5 exhibited prophylactic effects on BALB/c mice against fatal doses of highly pathogenic mouse-adapted influenza A subtypes (H1N1, H3N2, and H9N2). Before challenge with influenza virus, C. butyricum S-45-5-treated BALB/c mice showed increased levels of IFN-β, IFN-γ, IL-6, and IL-12 in serum, the small intestine, and bronchoalveolar lavage fluid (BALF), which correlated with observed prophylactic effects. Interestingly, after challenge with influenza virus, C. butyricum S-45-5-treated BALB/c mice showed reduced levels of pro-inflammatory cytokines and relatively higher levels of anti-inflammatory cytokines at day 7 post-infection. Taken together, these findings suggest that C. butyricum S-45-5 plays an antiviral role in vitro and in vivo by inducing an antiviral state and affects immune modulation to alleviate local and systemic inflammatory responses caused by influenza virus infection. Our study provides the beneficial effects of the new C. butyricum S-45-5 with antiviral effects as a probiotic.https://www.frontiersin.org/articles/10.3389/fimmu.2023.1242183/fullClostridium butyricum S-45-5probioticsantiviral activityinterferoninfluenza virus |
| spellingShingle | Kiramage Chathuranga Yeseul Shin Md Bashir Uddin Md Bashir Uddin Md Bashir Uddin Jayoung Paek W. A. Gayan Chathuranga Yebin Seong Lu Bai Hongik Kim Jeong Hwan Shin Young-Hyo Chang Jong-Soo Lee The novel immunobiotic Clostridium butyricum S-45-5 displays broad-spectrum antiviral activity in vitro and in vivo by inducing immune modulation Frontiers in Immunology Clostridium butyricum S-45-5 probiotics antiviral activity interferon influenza virus |
| title | The novel immunobiotic Clostridium butyricum S-45-5 displays broad-spectrum antiviral activity in vitro and in vivo by inducing immune modulation |
| title_full | The novel immunobiotic Clostridium butyricum S-45-5 displays broad-spectrum antiviral activity in vitro and in vivo by inducing immune modulation |
| title_fullStr | The novel immunobiotic Clostridium butyricum S-45-5 displays broad-spectrum antiviral activity in vitro and in vivo by inducing immune modulation |
| title_full_unstemmed | The novel immunobiotic Clostridium butyricum S-45-5 displays broad-spectrum antiviral activity in vitro and in vivo by inducing immune modulation |
| title_short | The novel immunobiotic Clostridium butyricum S-45-5 displays broad-spectrum antiviral activity in vitro and in vivo by inducing immune modulation |
| title_sort | novel immunobiotic clostridium butyricum s 45 5 displays broad spectrum antiviral activity in vitro and in vivo by inducing immune modulation |
| topic | Clostridium butyricum S-45-5 probiotics antiviral activity interferon influenza virus |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1242183/full |
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