Orf165 is associated with cytoplasmic male sterility in pepper
Abstract Cytoplasmic male sterility (CMS) is a maternally inherited trait that derives from the inability to produce functional pollen in higher plants. CMS results from recombination of the mitochondrial genome. However, understanding of the molecular mechanism of CMS in pepper is limited. In this...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
2021-09-01
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| Series: | Genetics and Molecular Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572021000400302&tlng=en |
| _version_ | 1818744620879708160 |
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| author | Jin-fen Wen Kai Zhao Jun-heng Lv Jin-long Huo Zi-ran Wang Hong-jian Wan Hai-shan Zhu Zhu-qing Zhang Gui-fang Shao Jiao Wang Shui Zhang Ting-yu Yang Jing-rou Zhang Xue-xiao Zou Ming-hua Deng |
| author_facet | Jin-fen Wen Kai Zhao Jun-heng Lv Jin-long Huo Zi-ran Wang Hong-jian Wan Hai-shan Zhu Zhu-qing Zhang Gui-fang Shao Jiao Wang Shui Zhang Ting-yu Yang Jing-rou Zhang Xue-xiao Zou Ming-hua Deng |
| author_sort | Jin-fen Wen |
| collection | DOAJ |
| description | Abstract Cytoplasmic male sterility (CMS) is a maternally inherited trait that derives from the inability to produce functional pollen in higher plants. CMS results from recombination of the mitochondrial genome. However, understanding of the molecular mechanism of CMS in pepper is limited. In this study, comparative transcriptomic analyses were performed using a near-isogenic CMS line 14A (CMS-14A) and a maintainer line 14B (ML-14B) as experimental materials. A total of 17,349 differentially expressed genes were detected between CMS-14A and ML-14B at the PMC meiosis stage. Among them, six unigenes associated with CMS and 108 unigenes involved in energy metabolism were identified. The gene orf165 was found in CMS-14A. When orf165 was introduced into ML-14B, almost 30% of transgenic plants were CMS. In addition, orf165 expression in transgenic CMS plants resulted in abnormal function of some genes involved in energy metabolism. When orf165 in transgenic CMS plant was silenced, the resulted orf165-silenced plant was male fertile and the expression patterns of some genes associated with energy metabolism were similar to ML-14B. Thus, we confirmed that orf165 influenced CMS in pepper. |
| first_indexed | 2024-12-18T02:47:13Z |
| format | Article |
| id | doaj.art-4a7d387632ed43d4a037b6dd210a470f |
| institution | Directory Open Access Journal |
| issn | 1678-4685 |
| language | English |
| last_indexed | 2024-12-18T02:47:13Z |
| publishDate | 2021-09-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-4a7d387632ed43d4a037b6dd210a470f2022-12-21T21:23:32ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1678-46852021-09-0144310.1590/1678-4685-gmb-2021-0030Orf165 is associated with cytoplasmic male sterility in pepperJin-fen WenKai ZhaoJun-heng LvJin-long HuoZi-ran WangHong-jian WanHai-shan ZhuZhu-qing ZhangGui-fang ShaoJiao WangShui ZhangTing-yu YangJing-rou ZhangXue-xiao ZouMing-hua Denghttps://orcid.org/0000-0001-8293-9035Abstract Cytoplasmic male sterility (CMS) is a maternally inherited trait that derives from the inability to produce functional pollen in higher plants. CMS results from recombination of the mitochondrial genome. However, understanding of the molecular mechanism of CMS in pepper is limited. In this study, comparative transcriptomic analyses were performed using a near-isogenic CMS line 14A (CMS-14A) and a maintainer line 14B (ML-14B) as experimental materials. A total of 17,349 differentially expressed genes were detected between CMS-14A and ML-14B at the PMC meiosis stage. Among them, six unigenes associated with CMS and 108 unigenes involved in energy metabolism were identified. The gene orf165 was found in CMS-14A. When orf165 was introduced into ML-14B, almost 30% of transgenic plants were CMS. In addition, orf165 expression in transgenic CMS plants resulted in abnormal function of some genes involved in energy metabolism. When orf165 in transgenic CMS plant was silenced, the resulted orf165-silenced plant was male fertile and the expression patterns of some genes associated with energy metabolism were similar to ML-14B. Thus, we confirmed that orf165 influenced CMS in pepper.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572021000400302&tlng=enCapsicum annuum L.comparative transcriptomic analysestransgenic plantsmitochondrial targetingVIGS |
| spellingShingle | Jin-fen Wen Kai Zhao Jun-heng Lv Jin-long Huo Zi-ran Wang Hong-jian Wan Hai-shan Zhu Zhu-qing Zhang Gui-fang Shao Jiao Wang Shui Zhang Ting-yu Yang Jing-rou Zhang Xue-xiao Zou Ming-hua Deng Orf165 is associated with cytoplasmic male sterility in pepper Genetics and Molecular Biology Capsicum annuum L. comparative transcriptomic analyses transgenic plants mitochondrial targeting VIGS |
| title | Orf165 is associated with cytoplasmic male sterility in pepper |
| title_full | Orf165 is associated with cytoplasmic male sterility in pepper |
| title_fullStr | Orf165 is associated with cytoplasmic male sterility in pepper |
| title_full_unstemmed | Orf165 is associated with cytoplasmic male sterility in pepper |
| title_short | Orf165 is associated with cytoplasmic male sterility in pepper |
| title_sort | orf165 is associated with cytoplasmic male sterility in pepper |
| topic | Capsicum annuum L. comparative transcriptomic analyses transgenic plants mitochondrial targeting VIGS |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572021000400302&tlng=en |
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