Oxygen availability is a major factor in determining the composition of microbial communities involved in methane oxidation
We have previously observed that methane supplied to lake sediment microbial communities as a substrate not only causes a response by bona fide methanotrophic bacteria, but also by non-methane-oxidizing bacteria, especially by members of the family Methylophilaceae. This result suggested that methan...
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| Format: | Article |
| Language: | English |
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PeerJ Inc.
2015-02-01
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| Online Access: | https://peerj.com/articles/801.pdf |
| _version_ | 1797422156025430016 |
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| author | Maria E. Hernandez David A.C. Beck Mary E. Lidstrom Ludmila Chistoserdova |
| author_facet | Maria E. Hernandez David A.C. Beck Mary E. Lidstrom Ludmila Chistoserdova |
| author_sort | Maria E. Hernandez |
| collection | DOAJ |
| description | We have previously observed that methane supplied to lake sediment microbial communities as a substrate not only causes a response by bona fide methanotrophic bacteria, but also by non-methane-oxidizing bacteria, especially by members of the family Methylophilaceae. This result suggested that methane oxidation in this environment likely involves communities composed of different functional guilds, rather than a single type of microbe. To obtain further support for this concept and to obtain further insights into the factors that may define such partnerships, we carried out microcosm incubations with sediment samples from Lake Washington at five different oxygen tensions, while methane was supplied at the same concentration in each. Community composition was determined through 16S rRNA gene amplicon sequencing after 10 and 16 weeks of incubation. We demonstrate that, in support of our prior observations, the methane-consuming communities were represented by two major types: the methanotrophs of the family Methylococcaceae and by non-methanotrophic methylotrophs of the family Methylophilaceae. However, different species persisted under different oxygen tensions. At high initial oxygen tensions (150 to 225 µM) the major players were, respectively, species of the genera Methylosarcina and Methylophilus, while at low initial oxygen tensions (15 to 75 µM) the major players were Methylobacter and Methylotenera. These data suggest that oxygen availability is at least one major factor determining specific partnerships in methane oxidation. The data also suggest that speciation within Methylococcaceae and Methylophilaceae may be driven by niche adaptation tailored toward specific placements within the oxygen gradient. |
| first_indexed | 2024-03-09T07:28:07Z |
| format | Article |
| id | doaj.art-4a7d9dd9ef37417fa6fa805ee2cae784 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T07:28:07Z |
| publishDate | 2015-02-01 |
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| spelling | doaj.art-4a7d9dd9ef37417fa6fa805ee2cae7842023-12-03T06:48:42ZengPeerJ Inc.PeerJ2167-83592015-02-013e80110.7717/peerj.801801Oxygen availability is a major factor in determining the composition of microbial communities involved in methane oxidationMaria E. Hernandez0David A.C. Beck1Mary E. Lidstrom2Ludmila Chistoserdova3Department of Chemical Engineering, University of Washington, Seattle, USADepartment of Chemical Engineering, University of Washington, Seattle, USADepartment of Chemical Engineering, University of Washington, Seattle, USADepartment of Chemical Engineering, University of Washington, Seattle, USAWe have previously observed that methane supplied to lake sediment microbial communities as a substrate not only causes a response by bona fide methanotrophic bacteria, but also by non-methane-oxidizing bacteria, especially by members of the family Methylophilaceae. This result suggested that methane oxidation in this environment likely involves communities composed of different functional guilds, rather than a single type of microbe. To obtain further support for this concept and to obtain further insights into the factors that may define such partnerships, we carried out microcosm incubations with sediment samples from Lake Washington at five different oxygen tensions, while methane was supplied at the same concentration in each. Community composition was determined through 16S rRNA gene amplicon sequencing after 10 and 16 weeks of incubation. We demonstrate that, in support of our prior observations, the methane-consuming communities were represented by two major types: the methanotrophs of the family Methylococcaceae and by non-methanotrophic methylotrophs of the family Methylophilaceae. However, different species persisted under different oxygen tensions. At high initial oxygen tensions (150 to 225 µM) the major players were, respectively, species of the genera Methylosarcina and Methylophilus, while at low initial oxygen tensions (15 to 75 µM) the major players were Methylobacter and Methylotenera. These data suggest that oxygen availability is at least one major factor determining specific partnerships in methane oxidation. The data also suggest that speciation within Methylococcaceae and Methylophilaceae may be driven by niche adaptation tailored toward specific placements within the oxygen gradient.https://peerj.com/articles/801.pdf Methylophilaceae Methanotroph Methylophilus Methylobacter Methylotenera Methylosarcina |
| spellingShingle | Maria E. Hernandez David A.C. Beck Mary E. Lidstrom Ludmila Chistoserdova Oxygen availability is a major factor in determining the composition of microbial communities involved in methane oxidation PeerJ Methylophilaceae Methanotroph Methylophilus Methylobacter Methylotenera Methylosarcina |
| title | Oxygen availability is a major factor in determining the composition of microbial communities involved in methane oxidation |
| title_full | Oxygen availability is a major factor in determining the composition of microbial communities involved in methane oxidation |
| title_fullStr | Oxygen availability is a major factor in determining the composition of microbial communities involved in methane oxidation |
| title_full_unstemmed | Oxygen availability is a major factor in determining the composition of microbial communities involved in methane oxidation |
| title_short | Oxygen availability is a major factor in determining the composition of microbial communities involved in methane oxidation |
| title_sort | oxygen availability is a major factor in determining the composition of microbial communities involved in methane oxidation |
| topic | Methylophilaceae Methanotroph Methylophilus Methylobacter Methylotenera Methylosarcina |
| url | https://peerj.com/articles/801.pdf |
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