Comparative transcriptomic profiling in the pulp and peel of pitaya fruit uncovers the gene networks regulating pulp color formation
Pitaya (genus Hylocereus) is a popular fruit. To develop pitaya fruit with greater marketability and high nutritional value, it is important to elucidate the roles of candidate genes and key metabolites that contribute to the coloration of the pitaya pulp and peel. By combining transcriptome and bio...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.968925/full |
| _version_ | 1818010855038517248 |
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| author | Xiaomei Li Yayuan Tang Yayuan Tang Li Li Li Li Guidong Liang Jing Li Chaoan Liu Xuemei He Xuemei He Jian Sun |
| author_facet | Xiaomei Li Yayuan Tang Yayuan Tang Li Li Li Li Guidong Liang Jing Li Chaoan Liu Xuemei He Xuemei He Jian Sun |
| author_sort | Xiaomei Li |
| collection | DOAJ |
| description | Pitaya (genus Hylocereus) is a popular fruit. To develop pitaya fruit with greater marketability and high nutritional value, it is important to elucidate the roles of candidate genes and key metabolites that contribute to the coloration of the pitaya pulp and peel. By combining transcriptome and biochemical analyses, we compared and analyzed the dynamic changes in the peel and pulp of H. undatus (white pulp) and H. polyrhizus (red pulp) fruits at four key time points during ripening. Differential expression analysis and temporal analysis revealed the difference regulation in pathways of plant hormone signal transduction, phenylpropanoid biosynthesis, and betalain biosynthesis. Our results suggest that color formation of purple-red peel and pulp of pitaya is influenced by betalains. Increased tyrosine content and fluctuation in acylated betalain content may be responsible for pulp color formation, while some of the key genes in this network showed differential expression patterns during ripening between white pulp and red pulp fruits. The data and analysis results of this study provide theoretical basis for the red color formation mechanism of pitaya, which will facilitate future work to improve pitaya fruit physical appearance and marketability. |
| first_indexed | 2024-04-14T06:00:46Z |
| format | Article |
| id | doaj.art-4a7eac12ec0c49df8042157fe798b092 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-14T06:00:46Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-4a7eac12ec0c49df8042157fe798b0922022-12-22T02:08:46ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-08-011310.3389/fpls.2022.968925968925Comparative transcriptomic profiling in the pulp and peel of pitaya fruit uncovers the gene networks regulating pulp color formationXiaomei Li0Yayuan Tang1Yayuan Tang2Li Li3Li Li4Guidong Liang5Jing Li6Chaoan Liu7Xuemei He8Xuemei He9Jian Sun10College of Chemistry and Bioengineering, Guilin University of Technology, Guilin, ChinaAgro-food Science and Technology Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, ChinaGuangxi Key Laboratory of Fruits and Vegetables Storage-processing Technology, Nanning, ChinaAgro-food Science and Technology Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, ChinaGuangxi Key Laboratory of Fruits and Vegetables Storage-processing Technology, Nanning, ChinaHorticultural Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, ChinaCollege of Chemistry and Bioengineering, Guilin University of Technology, Guilin, ChinaHorticultural Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, ChinaAgro-food Science and Technology Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, ChinaGuangxi Key Laboratory of Fruits and Vegetables Storage-processing Technology, Nanning, ChinaGuangxi Academy of Agricultural Sciences, Nanning, ChinaPitaya (genus Hylocereus) is a popular fruit. To develop pitaya fruit with greater marketability and high nutritional value, it is important to elucidate the roles of candidate genes and key metabolites that contribute to the coloration of the pitaya pulp and peel. By combining transcriptome and biochemical analyses, we compared and analyzed the dynamic changes in the peel and pulp of H. undatus (white pulp) and H. polyrhizus (red pulp) fruits at four key time points during ripening. Differential expression analysis and temporal analysis revealed the difference regulation in pathways of plant hormone signal transduction, phenylpropanoid biosynthesis, and betalain biosynthesis. Our results suggest that color formation of purple-red peel and pulp of pitaya is influenced by betalains. Increased tyrosine content and fluctuation in acylated betalain content may be responsible for pulp color formation, while some of the key genes in this network showed differential expression patterns during ripening between white pulp and red pulp fruits. The data and analysis results of this study provide theoretical basis for the red color formation mechanism of pitaya, which will facilitate future work to improve pitaya fruit physical appearance and marketability.https://www.frontiersin.org/articles/10.3389/fpls.2022.968925/fullpitaya fruitpulp color formationred peeltranscriptomegene network |
| spellingShingle | Xiaomei Li Yayuan Tang Yayuan Tang Li Li Li Li Guidong Liang Jing Li Chaoan Liu Xuemei He Xuemei He Jian Sun Comparative transcriptomic profiling in the pulp and peel of pitaya fruit uncovers the gene networks regulating pulp color formation Frontiers in Plant Science pitaya fruit pulp color formation red peel transcriptome gene network |
| title | Comparative transcriptomic profiling in the pulp and peel of pitaya fruit uncovers the gene networks regulating pulp color formation |
| title_full | Comparative transcriptomic profiling in the pulp and peel of pitaya fruit uncovers the gene networks regulating pulp color formation |
| title_fullStr | Comparative transcriptomic profiling in the pulp and peel of pitaya fruit uncovers the gene networks regulating pulp color formation |
| title_full_unstemmed | Comparative transcriptomic profiling in the pulp and peel of pitaya fruit uncovers the gene networks regulating pulp color formation |
| title_short | Comparative transcriptomic profiling in the pulp and peel of pitaya fruit uncovers the gene networks regulating pulp color formation |
| title_sort | comparative transcriptomic profiling in the pulp and peel of pitaya fruit uncovers the gene networks regulating pulp color formation |
| topic | pitaya fruit pulp color formation red peel transcriptome gene network |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.968925/full |
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