Multidrug-Resistant and Genetic Characterization of Extended-Spectrum Beta-Lactamase-Producing <i>E. coli</i> Recovered from Chickens and Humans in Egypt
Colonization of food chain animals such as chickens with extended-spectrum β-lactamases (ESBL) poses a major health threat to human. The current study aimed to determine the phenotypic and genotypic relationship between ESBL-producing <i>E. coli</i> from diseased human and chickens in Eg...
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| Format: | Article |
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MDPI AG
2022-01-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/12/3/346 |
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| author | Heba Badr Reem M. Reda Naglaa M. Hagag Essam Kamel Sara M. Elnomrosy Amal I. Mansour Momtaz A. Shahein Samah F. Ali Hala R. Ali |
| author_facet | Heba Badr Reem M. Reda Naglaa M. Hagag Essam Kamel Sara M. Elnomrosy Amal I. Mansour Momtaz A. Shahein Samah F. Ali Hala R. Ali |
| author_sort | Heba Badr |
| collection | DOAJ |
| description | Colonization of food chain animals such as chickens with extended-spectrum β-lactamases (ESBL) poses a major health threat to human. The current study aimed to determine the phenotypic and genotypic relationship between ESBL-producing <i>E. coli</i> from diseased human and chickens in Egypt. A total of 56 out of 120 chicken farms (46.7%) and 9 human samples (100%) were phenotypically and genotypically identified with at least one ESBL-phenotype/gene. Chicken isolates showed a high proportion of beta lactamase from CTX-M group 9 > TEM > PER families, followed by CTX-M group 1 > SHV > GES > OXA group10 > VEB > OXA group2 families, while human isolates only contained the CTX-M family. A high incidence of ESBL genes from the CTX-M family was recognized in both human and chicken isolates. Furthermore, nucleotide identity showed high similarity between chicken and human isolates. In conclusion, the current study traced phenotypes and genotypes of ESBL-producing <i>E. coli</i> from chickens and human samples in Egypt, reporting degrees of similarity that suggest potential zoonotic transmission. Our data highlighted the significant importance of chicken as a major food source not only in Egypt but all over the world in the spreading of ESBL-producing <i>E. coli</i> to human. |
| first_indexed | 2024-03-10T00:17:49Z |
| format | Article |
| id | doaj.art-4a80d47a8e904ab494178f6dce14dec7 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T00:17:49Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-4a80d47a8e904ab494178f6dce14dec72023-11-23T15:48:18ZengMDPI AGAnimals2076-26152022-01-0112334610.3390/ani12030346Multidrug-Resistant and Genetic Characterization of Extended-Spectrum Beta-Lactamase-Producing <i>E. coli</i> Recovered from Chickens and Humans in EgyptHeba Badr0Reem M. Reda1Naglaa M. Hagag2Essam Kamel3Sara M. Elnomrosy4Amal I. Mansour5Momtaz A. Shahein6Samah F. Ali7Hala R. Ali8Reference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Agriculture Research Center (ARC), Giza 12618, EgyptReference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Agriculture Research Center (ARC), Giza 12618, EgyptReference Laboratory for Veterinary Quality Control on Poultry Production, Animal Health Research Institute, Agriculture Research Center (ARC), Giza 12618, EgyptDepartment of chemistry, Animal Health Research Institute, Agriculture Research Center (ARC), Giza 12618, EgyptGenome Research Unit, Animal Health Research Institute, Agriculture Research Center (ARC), Giza 12618, EgyptBacteriology, Food and Agriculture Organization of the United Nations, Giza 2223, EgyptDepartment of Virology Research, Animal Health Research Institute, Agriculture Research Center (ARC), Giza 12618, EgyptBacteriology Department, Animal Health Research Institute (AHRI), Agriculture Research Center (ARC), Giza 12618, EgyptBacteriology Department, Animal Health Research Institute (AHRI), Agriculture Research Center (ARC), Giza 12618, EgyptColonization of food chain animals such as chickens with extended-spectrum β-lactamases (ESBL) poses a major health threat to human. The current study aimed to determine the phenotypic and genotypic relationship between ESBL-producing <i>E. coli</i> from diseased human and chickens in Egypt. A total of 56 out of 120 chicken farms (46.7%) and 9 human samples (100%) were phenotypically and genotypically identified with at least one ESBL-phenotype/gene. Chicken isolates showed a high proportion of beta lactamase from CTX-M group 9 > TEM > PER families, followed by CTX-M group 1 > SHV > GES > OXA group10 > VEB > OXA group2 families, while human isolates only contained the CTX-M family. A high incidence of ESBL genes from the CTX-M family was recognized in both human and chicken isolates. Furthermore, nucleotide identity showed high similarity between chicken and human isolates. In conclusion, the current study traced phenotypes and genotypes of ESBL-producing <i>E. coli</i> from chickens and human samples in Egypt, reporting degrees of similarity that suggest potential zoonotic transmission. Our data highlighted the significant importance of chicken as a major food source not only in Egypt but all over the world in the spreading of ESBL-producing <i>E. coli</i> to human.https://www.mdpi.com/2076-2615/12/3/346antibiotic resistance<i>E. coli</i>ESBLpoultryhuman |
| spellingShingle | Heba Badr Reem M. Reda Naglaa M. Hagag Essam Kamel Sara M. Elnomrosy Amal I. Mansour Momtaz A. Shahein Samah F. Ali Hala R. Ali Multidrug-Resistant and Genetic Characterization of Extended-Spectrum Beta-Lactamase-Producing <i>E. coli</i> Recovered from Chickens and Humans in Egypt Animals antibiotic resistance <i>E. coli</i> ESBL poultry human |
| title | Multidrug-Resistant and Genetic Characterization of Extended-Spectrum Beta-Lactamase-Producing <i>E. coli</i> Recovered from Chickens and Humans in Egypt |
| title_full | Multidrug-Resistant and Genetic Characterization of Extended-Spectrum Beta-Lactamase-Producing <i>E. coli</i> Recovered from Chickens and Humans in Egypt |
| title_fullStr | Multidrug-Resistant and Genetic Characterization of Extended-Spectrum Beta-Lactamase-Producing <i>E. coli</i> Recovered from Chickens and Humans in Egypt |
| title_full_unstemmed | Multidrug-Resistant and Genetic Characterization of Extended-Spectrum Beta-Lactamase-Producing <i>E. coli</i> Recovered from Chickens and Humans in Egypt |
| title_short | Multidrug-Resistant and Genetic Characterization of Extended-Spectrum Beta-Lactamase-Producing <i>E. coli</i> Recovered from Chickens and Humans in Egypt |
| title_sort | multidrug resistant and genetic characterization of extended spectrum beta lactamase producing i e coli i recovered from chickens and humans in egypt |
| topic | antibiotic resistance <i>E. coli</i> ESBL poultry human |
| url | https://www.mdpi.com/2076-2615/12/3/346 |
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