Impact of frequency of denture cleaning on microbial and clinical parameters – a bench to chairside approach

Objective: Robust scientific and clinical evidence of how to appropriately manage denture plaque is lacking. This two-part study (i) developed an in vitro model of denture plaque removal, and (ii) assessed effectiveness of these approaches in a randomised clinical trial. Method: (i) a complex dentur...

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Bibliographic Details
Main Authors: Gordon Ramage, Lindsay O’Donnell, Leighann Sherry, Shauna Culshaw, Jeremy Bagg, Marta Czesnikiewicz-Guzik, Clare Brown, Debbie McKenzie, Laura Cross, Andrew MacInnes, David Bradshaw, Roshan Varghese, Paola Gomez Pereira, Anto Jose, Susmita Sanyal, Douglas Robertson
Format: Article
Language:English
Published: Taylor & Francis Group 2019-01-01
Series:Journal of Oral Microbiology
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Online Access:http://dx.doi.org/10.1080/20002297.2018.1538437
Description
Summary:Objective: Robust scientific and clinical evidence of how to appropriately manage denture plaque is lacking. This two-part study (i) developed an in vitro model of denture plaque removal, and (ii) assessed effectiveness of these approaches in a randomised clinical trial. Method: (i) a complex denture plaque model was developed using the dominant microbial genera from a recent microbiome analyses. Biofilms formed on polymethylmethacrylate were brushed daily with a wet toothbrush, then either treated daily for 5 days or only on Days 1 and 5 with Polident® denture cleanser tablets (3 min soaking). Quantitative and qualitative microbiological assessments were performed. (ii), an examiner-blind, randomised, crossover study of complete maxillary denture wearers was performed (n = 19). Either once-daily for 7 days or on Day 7 only, participants soaked dentures for 15 min using Corega® denture cleansing tables, then brushed. Denture plaque microbiological assessment used sterilized filter paper discs. Results: The in vitro model showed daily cleaning with denture cleanser plus brushing significantly reduced microbial numbers compared to intermittent denture cleaning with daily brushing (p < 0.001). The clinical component of the study showed a statistically significant reduction in denture plaque microbial numbers in favour of daily versus weekly treatment (aerobic bacteria p = 0.0144). Both in vitro and in vivo studies showed that denture plaque biofilm composition were affected by different treatment arms. Conclusions: This study demonstrated that daily denture cleansing regimens are superior to intermittent denture cleansing, and that cleansing regimens can induce denture plaque compositional changes. Trial registration: ClinicalTrials.gov identifier: NCT02780661.
ISSN:2000-2297