Impact of frequency of denture cleaning on microbial and clinical parameters – a bench to chairside approach
Objective: Robust scientific and clinical evidence of how to appropriately manage denture plaque is lacking. This two-part study (i) developed an in vitro model of denture plaque removal, and (ii) assessed effectiveness of these approaches in a randomised clinical trial. Method: (i) a complex dentur...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-01-01
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| Series: | Journal of Oral Microbiology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/20002297.2018.1538437 |
| _version_ | 1819171863533715456 |
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| author | Gordon Ramage Lindsay O’Donnell Leighann Sherry Shauna Culshaw Jeremy Bagg Marta Czesnikiewicz-Guzik Clare Brown Debbie McKenzie Laura Cross Andrew MacInnes David Bradshaw Roshan Varghese Paola Gomez Pereira Anto Jose Susmita Sanyal Douglas Robertson |
| author_facet | Gordon Ramage Lindsay O’Donnell Leighann Sherry Shauna Culshaw Jeremy Bagg Marta Czesnikiewicz-Guzik Clare Brown Debbie McKenzie Laura Cross Andrew MacInnes David Bradshaw Roshan Varghese Paola Gomez Pereira Anto Jose Susmita Sanyal Douglas Robertson |
| author_sort | Gordon Ramage |
| collection | DOAJ |
| description | Objective: Robust scientific and clinical evidence of how to appropriately manage denture plaque is lacking. This two-part study (i) developed an in vitro model of denture plaque removal, and (ii) assessed effectiveness of these approaches in a randomised clinical trial. Method: (i) a complex denture plaque model was developed using the dominant microbial genera from a recent microbiome analyses. Biofilms formed on polymethylmethacrylate were brushed daily with a wet toothbrush, then either treated daily for 5 days or only on Days 1 and 5 with Polident® denture cleanser tablets (3 min soaking). Quantitative and qualitative microbiological assessments were performed. (ii), an examiner-blind, randomised, crossover study of complete maxillary denture wearers was performed (n = 19). Either once-daily for 7 days or on Day 7 only, participants soaked dentures for 15 min using Corega® denture cleansing tables, then brushed. Denture plaque microbiological assessment used sterilized filter paper discs. Results: The in vitro model showed daily cleaning with denture cleanser plus brushing significantly reduced microbial numbers compared to intermittent denture cleaning with daily brushing (p < 0.001). The clinical component of the study showed a statistically significant reduction in denture plaque microbial numbers in favour of daily versus weekly treatment (aerobic bacteria p = 0.0144). Both in vitro and in vivo studies showed that denture plaque biofilm composition were affected by different treatment arms. Conclusions: This study demonstrated that daily denture cleansing regimens are superior to intermittent denture cleansing, and that cleansing regimens can induce denture plaque compositional changes. Trial registration: ClinicalTrials.gov identifier: NCT02780661. |
| first_indexed | 2024-12-22T19:58:03Z |
| format | Article |
| id | doaj.art-4a811b8319d94b38b032c7282428168e |
| institution | Directory Open Access Journal |
| issn | 2000-2297 |
| language | English |
| last_indexed | 2024-12-22T19:58:03Z |
| publishDate | 2019-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Oral Microbiology |
| spelling | doaj.art-4a811b8319d94b38b032c7282428168e2022-12-21T18:14:22ZengTaylor & Francis GroupJournal of Oral Microbiology2000-22972019-01-0111110.1080/20002297.2018.15384371538437Impact of frequency of denture cleaning on microbial and clinical parameters – a bench to chairside approachGordon Ramage0Lindsay O’Donnell1Leighann Sherry2Shauna Culshaw3Jeremy Bagg4Marta Czesnikiewicz-Guzik5Clare Brown6Debbie McKenzie7Laura Cross8Andrew MacInnes9David Bradshaw10Roshan Varghese11Paola Gomez Pereira12Anto Jose13Susmita Sanyal14Douglas Robertson15University of GlasgowUniversity of GlasgowUniversity of GlasgowUniversity of GlasgowUniversity of GlasgowUniversity of GlasgowUniversity of GlasgowUniversity of GlasgowUniversity of GlasgowUniversity of GlasgowGSK Consumer HealthcareGSK Consumer HealthcareGSK Consumer HealthcareGSK Consumer HealthcareSyneos HealthUniversity of GlasgowObjective: Robust scientific and clinical evidence of how to appropriately manage denture plaque is lacking. This two-part study (i) developed an in vitro model of denture plaque removal, and (ii) assessed effectiveness of these approaches in a randomised clinical trial. Method: (i) a complex denture plaque model was developed using the dominant microbial genera from a recent microbiome analyses. Biofilms formed on polymethylmethacrylate were brushed daily with a wet toothbrush, then either treated daily for 5 days or only on Days 1 and 5 with Polident® denture cleanser tablets (3 min soaking). Quantitative and qualitative microbiological assessments were performed. (ii), an examiner-blind, randomised, crossover study of complete maxillary denture wearers was performed (n = 19). Either once-daily for 7 days or on Day 7 only, participants soaked dentures for 15 min using Corega® denture cleansing tables, then brushed. Denture plaque microbiological assessment used sterilized filter paper discs. Results: The in vitro model showed daily cleaning with denture cleanser plus brushing significantly reduced microbial numbers compared to intermittent denture cleaning with daily brushing (p < 0.001). The clinical component of the study showed a statistically significant reduction in denture plaque microbial numbers in favour of daily versus weekly treatment (aerobic bacteria p = 0.0144). Both in vitro and in vivo studies showed that denture plaque biofilm composition were affected by different treatment arms. Conclusions: This study demonstrated that daily denture cleansing regimens are superior to intermittent denture cleansing, and that cleansing regimens can induce denture plaque compositional changes. Trial registration: ClinicalTrials.gov identifier: NCT02780661.http://dx.doi.org/10.1080/20002297.2018.1538437dentureplaquemicrobiologydenture cleansingoral microbiomeantimicrobials |
| spellingShingle | Gordon Ramage Lindsay O’Donnell Leighann Sherry Shauna Culshaw Jeremy Bagg Marta Czesnikiewicz-Guzik Clare Brown Debbie McKenzie Laura Cross Andrew MacInnes David Bradshaw Roshan Varghese Paola Gomez Pereira Anto Jose Susmita Sanyal Douglas Robertson Impact of frequency of denture cleaning on microbial and clinical parameters – a bench to chairside approach Journal of Oral Microbiology denture plaque microbiology denture cleansing oral microbiome antimicrobials |
| title | Impact of frequency of denture cleaning on microbial and clinical parameters – a bench to chairside approach |
| title_full | Impact of frequency of denture cleaning on microbial and clinical parameters – a bench to chairside approach |
| title_fullStr | Impact of frequency of denture cleaning on microbial and clinical parameters – a bench to chairside approach |
| title_full_unstemmed | Impact of frequency of denture cleaning on microbial and clinical parameters – a bench to chairside approach |
| title_short | Impact of frequency of denture cleaning on microbial and clinical parameters – a bench to chairside approach |
| title_sort | impact of frequency of denture cleaning on microbial and clinical parameters a bench to chairside approach |
| topic | denture plaque microbiology denture cleansing oral microbiome antimicrobials |
| url | http://dx.doi.org/10.1080/20002297.2018.1538437 |
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