Exogenous <i>Penicillium camemberti</i> Lipase Preparation Exerts Prebiotic-like Effects by Increasing Cecal <i>Bifidobacterium</i> and <i>Lactobacillus</i> Abundance in Rats
<i>Penicillium camemberti</i> is used in cheese production; however, its health benefits remain to be elucidated. We previously found that supplemental <i>Aspergillus</i>-derived lipase preparation exerts a strong bifidogenic effect in rats fed a high-fat diet. This study inv...
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| Format: | Article |
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MDPI AG
2023-02-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/9/3/227 |
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| author | Yongshou Yang Huijuan Jia Chika Ando Hisanori Kato Thanutchaporn Kumrungsee Norihisa Kato Akiko Kimoto Shinji Fukuda Manabu Kuroda Kyoichi Nishio Shotaro Yamaguchi |
| author_facet | Yongshou Yang Huijuan Jia Chika Ando Hisanori Kato Thanutchaporn Kumrungsee Norihisa Kato Akiko Kimoto Shinji Fukuda Manabu Kuroda Kyoichi Nishio Shotaro Yamaguchi |
| author_sort | Yongshou Yang |
| collection | DOAJ |
| description | <i>Penicillium camemberti</i> is used in cheese production; however, its health benefits remain to be elucidated. We previously found that supplemental <i>Aspergillus</i>-derived lipase preparation exerts a strong bifidogenic effect in rats fed a high-fat diet. This study investigated the effects of the feeding of a diet containing a 0.2% or 0.4% <i>Penicillium camemberti</i>-derived lipase preparation (PCL) for two weeks on the cecal microbiota in rats. According to 16S rRNA gene sequencing analysis, both PCL supplements significantly (<i>p</i> < 0.05) affected the cecal microbial community. At the genus level, supplemental 0.4% PCL significantly increased the relative abundance of beneficial bacteria such as <i>Bifidobacterium</i>, <i>Lactobacillus</i>, and <i>Collinsella</i> (127-fold, 6-fold, and 193-fold increase, respectively). The abundance of these bacteria in the 0.2% PCL group was between that of the control and 0.4% PCL groups. Notably, the effects of supplemental 0.4% PCL on modulating the abundance of these bacteria matched the effects observed in studies on typical prebiotic oligosaccharides. PICRUSt analysis revealed that PCL supplements significantly modulated the relative abundance of bacterial genes associated with 27 metabolic pathways, some of which were similar to those reported for prebiotic oligosaccharides. This study provides the first evidence indicating that supplemental PCL exerts prebiotic-like effects by modulating the abundance of the gut microbiota. |
| first_indexed | 2024-03-11T06:33:49Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-11T06:33:49Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-4a873361e30144eb8a2b53c05ced1b4f2023-11-17T11:01:45ZengMDPI AGFermentation2311-56372023-02-019322710.3390/fermentation9030227Exogenous <i>Penicillium camemberti</i> Lipase Preparation Exerts Prebiotic-like Effects by Increasing Cecal <i>Bifidobacterium</i> and <i>Lactobacillus</i> Abundance in RatsYongshou Yang0Huijuan Jia1Chika Ando2Hisanori Kato3Thanutchaporn Kumrungsee4Norihisa Kato5Akiko Kimoto6Shinji Fukuda7Manabu Kuroda8Kyoichi Nishio9Shotaro Yamaguchi10School of Life Sciences, Anhui University, Hefei 230601, ChinaGraduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, JapanGraduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, JapanGraduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, JapanGraduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima 739-8528, JapanGraduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima 739-8528, JapanThe Faculty of Health Sciences, Hiroshima Shudo University, Hiroshima 731-3195, JapanInstitute for Advanced Biosciences, Keio University, Tsuruoka 997-0052, JapanAmano Enzyme Inc., Nagoya 460-8630, JapanAmano Enzyme Inc., Nagoya 460-8630, JapanAmano Enzyme Inc., Nagoya 460-8630, Japan<i>Penicillium camemberti</i> is used in cheese production; however, its health benefits remain to be elucidated. We previously found that supplemental <i>Aspergillus</i>-derived lipase preparation exerts a strong bifidogenic effect in rats fed a high-fat diet. This study investigated the effects of the feeding of a diet containing a 0.2% or 0.4% <i>Penicillium camemberti</i>-derived lipase preparation (PCL) for two weeks on the cecal microbiota in rats. According to 16S rRNA gene sequencing analysis, both PCL supplements significantly (<i>p</i> < 0.05) affected the cecal microbial community. At the genus level, supplemental 0.4% PCL significantly increased the relative abundance of beneficial bacteria such as <i>Bifidobacterium</i>, <i>Lactobacillus</i>, and <i>Collinsella</i> (127-fold, 6-fold, and 193-fold increase, respectively). The abundance of these bacteria in the 0.2% PCL group was between that of the control and 0.4% PCL groups. Notably, the effects of supplemental 0.4% PCL on modulating the abundance of these bacteria matched the effects observed in studies on typical prebiotic oligosaccharides. PICRUSt analysis revealed that PCL supplements significantly modulated the relative abundance of bacterial genes associated with 27 metabolic pathways, some of which were similar to those reported for prebiotic oligosaccharides. This study provides the first evidence indicating that supplemental PCL exerts prebiotic-like effects by modulating the abundance of the gut microbiota.https://www.mdpi.com/2311-5637/9/3/227<i>Penicillium camemberti</i>lipase16S rRNAprebiotics<i>Bifidobacterium</i><i>Lactobacillus</i> |
| spellingShingle | Yongshou Yang Huijuan Jia Chika Ando Hisanori Kato Thanutchaporn Kumrungsee Norihisa Kato Akiko Kimoto Shinji Fukuda Manabu Kuroda Kyoichi Nishio Shotaro Yamaguchi Exogenous <i>Penicillium camemberti</i> Lipase Preparation Exerts Prebiotic-like Effects by Increasing Cecal <i>Bifidobacterium</i> and <i>Lactobacillus</i> Abundance in Rats Fermentation <i>Penicillium camemberti</i> lipase 16S rRNA prebiotics <i>Bifidobacterium</i> <i>Lactobacillus</i> |
| title | Exogenous <i>Penicillium camemberti</i> Lipase Preparation Exerts Prebiotic-like Effects by Increasing Cecal <i>Bifidobacterium</i> and <i>Lactobacillus</i> Abundance in Rats |
| title_full | Exogenous <i>Penicillium camemberti</i> Lipase Preparation Exerts Prebiotic-like Effects by Increasing Cecal <i>Bifidobacterium</i> and <i>Lactobacillus</i> Abundance in Rats |
| title_fullStr | Exogenous <i>Penicillium camemberti</i> Lipase Preparation Exerts Prebiotic-like Effects by Increasing Cecal <i>Bifidobacterium</i> and <i>Lactobacillus</i> Abundance in Rats |
| title_full_unstemmed | Exogenous <i>Penicillium camemberti</i> Lipase Preparation Exerts Prebiotic-like Effects by Increasing Cecal <i>Bifidobacterium</i> and <i>Lactobacillus</i> Abundance in Rats |
| title_short | Exogenous <i>Penicillium camemberti</i> Lipase Preparation Exerts Prebiotic-like Effects by Increasing Cecal <i>Bifidobacterium</i> and <i>Lactobacillus</i> Abundance in Rats |
| title_sort | exogenous i penicillium camemberti i lipase preparation exerts prebiotic like effects by increasing cecal i bifidobacterium i and i lactobacillus i abundance in rats |
| topic | <i>Penicillium camemberti</i> lipase 16S rRNA prebiotics <i>Bifidobacterium</i> <i>Lactobacillus</i> |
| url | https://www.mdpi.com/2311-5637/9/3/227 |
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