Epidemiological and genetic characteristics of porcine circovirus 3 in 15 provinces and municipalities of China between 2016 and 2020
Abstract Porcine circovirus 3 (PCV3) is a newly emerging virus and has been found associated with porcine dermatitis and nephropathy syndrome in pigs. Compared with PCV2, research into PCV3 cap gene sequencing is deficient. To investigate the prevalence and genotype distribution of PCV3, we collecte...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-11-01
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| Series: | Virology Journal |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12985-022-01893-0 |
| _version_ | 1797983887807217664 |
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| author | Xugang Ku Chengjun Zhang Panpan Li Xuexiang Yu Qi Sun Fengqin Xu Ping Qian Qigai He |
| author_facet | Xugang Ku Chengjun Zhang Panpan Li Xuexiang Yu Qi Sun Fengqin Xu Ping Qian Qigai He |
| author_sort | Xugang Ku |
| collection | DOAJ |
| description | Abstract Porcine circovirus 3 (PCV3) is a newly emerging virus and has been found associated with porcine dermatitis and nephropathy syndrome in pigs. Compared with PCV2, research into PCV3 cap gene sequencing is deficient. To investigate the prevalence and genotype distribution of PCV3, we collected 1291 samples from 211 pig farms throughout 15 provinces and municipalities. 312 out of 1291 samples were tested positive by PCR. We further sequenced and analyzed 164 PCR-positive samples. The majority (61.8%) of isolates we sequenced belong to genotype PCV3c. PCV3c is also the dominant genotype in Hubei, Hunan, Hebei province and Chongqing city. We found 3 sites under positive selection and located in predicted epitope peptide, revealing that the pig’s immunity may be a reason those sites are undergoing highly positive selection. |
| first_indexed | 2024-04-11T06:54:28Z |
| format | Article |
| id | doaj.art-4a8783eeb15a457784df811d226084f9 |
| institution | Directory Open Access Journal |
| issn | 1743-422X |
| language | English |
| last_indexed | 2024-04-11T06:54:28Z |
| publishDate | 2022-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj.art-4a8783eeb15a457784df811d226084f92022-12-22T04:39:05ZengBMCVirology Journal1743-422X2022-11-011911510.1186/s12985-022-01893-0Epidemiological and genetic characteristics of porcine circovirus 3 in 15 provinces and municipalities of China between 2016 and 2020Xugang Ku0Chengjun Zhang1Panpan Li2Xuexiang Yu3Qi Sun4Fengqin Xu5Ping Qian6Qigai He7State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural UniversityState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural UniversityThe Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural UniversityState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural UniversityState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural UniversityThe Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural UniversityState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural UniversityState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural UniversityAbstract Porcine circovirus 3 (PCV3) is a newly emerging virus and has been found associated with porcine dermatitis and nephropathy syndrome in pigs. Compared with PCV2, research into PCV3 cap gene sequencing is deficient. To investigate the prevalence and genotype distribution of PCV3, we collected 1291 samples from 211 pig farms throughout 15 provinces and municipalities. 312 out of 1291 samples were tested positive by PCR. We further sequenced and analyzed 164 PCR-positive samples. The majority (61.8%) of isolates we sequenced belong to genotype PCV3c. PCV3c is also the dominant genotype in Hubei, Hunan, Hebei province and Chongqing city. We found 3 sites under positive selection and located in predicted epitope peptide, revealing that the pig’s immunity may be a reason those sites are undergoing highly positive selection.https://doi.org/10.1186/s12985-022-01893-0Porcine circovirus 3Phylogenetic analysisSequence alignmentPCR detection |
| spellingShingle | Xugang Ku Chengjun Zhang Panpan Li Xuexiang Yu Qi Sun Fengqin Xu Ping Qian Qigai He Epidemiological and genetic characteristics of porcine circovirus 3 in 15 provinces and municipalities of China between 2016 and 2020 Virology Journal Porcine circovirus 3 Phylogenetic analysis Sequence alignment PCR detection |
| title | Epidemiological and genetic characteristics of porcine circovirus 3 in 15 provinces and municipalities of China between 2016 and 2020 |
| title_full | Epidemiological and genetic characteristics of porcine circovirus 3 in 15 provinces and municipalities of China between 2016 and 2020 |
| title_fullStr | Epidemiological and genetic characteristics of porcine circovirus 3 in 15 provinces and municipalities of China between 2016 and 2020 |
| title_full_unstemmed | Epidemiological and genetic characteristics of porcine circovirus 3 in 15 provinces and municipalities of China between 2016 and 2020 |
| title_short | Epidemiological and genetic characteristics of porcine circovirus 3 in 15 provinces and municipalities of China between 2016 and 2020 |
| title_sort | epidemiological and genetic characteristics of porcine circovirus 3 in 15 provinces and municipalities of china between 2016 and 2020 |
| topic | Porcine circovirus 3 Phylogenetic analysis Sequence alignment PCR detection |
| url | https://doi.org/10.1186/s12985-022-01893-0 |
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