Natural Sulfurs Inhibit LPS-Induced Inflammatory Responses through NF-κB Signaling in CCD-986Sk Skin Fibroblasts
Lipopolysaccharide (LPS)-induced inflammatory response leads to serious damage, up to and including tumorigenesis. Natural mineral sulfur, non-toxic sulfur (NTS), and methylsulfonylmethane (MSM) have anti-inflammatory activity that may inhibit LPS-induced inflammation. We hypothesized that sulfur co...
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MDPI AG
2021-05-01
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| Online Access: | https://www.mdpi.com/2075-1729/11/5/427 |
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| author | Nipin Sp Dong Young Kang Hyoung Do Kim Alexis Rugamba Eun Seong Jo Jong-Chan Park Se Won Bae Jin-Moo Lee Kyoung-Jin Jang |
| author_facet | Nipin Sp Dong Young Kang Hyoung Do Kim Alexis Rugamba Eun Seong Jo Jong-Chan Park Se Won Bae Jin-Moo Lee Kyoung-Jin Jang |
| author_sort | Nipin Sp |
| collection | DOAJ |
| description | Lipopolysaccharide (LPS)-induced inflammatory response leads to serious damage, up to and including tumorigenesis. Natural mineral sulfur, non-toxic sulfur (NTS), and methylsulfonylmethane (MSM) have anti-inflammatory activity that may inhibit LPS-induced inflammation. We hypothesized that sulfur compounds could inhibit LPS-induced inflammatory responses in CCD-986Sk skin fibroblasts. We used Western blotting and real-time PCR to analyze molecular signaling in treated and untreated cultures. We also used flow cytometry for cell surface receptor analysis, comet assays to evaluate DNA damage, and ELISA-based cytokine detection. LPS induced TLR4 activation and NF-κB signaling via canonical and protein kinase C (PKC)-dependent pathways, while NTS and MSM downregulated that response. NTS and MSM also inhibited LPS-induced nuclear accumulation and binding of NF-κB to proinflammatory cytokines COX-2, IL-1β, and IL-6. Finally, the sulfur compounds suppressed LPS-induced ROS accumulation and DNA damage in CCD-986Sk cells. These results suggest that natural sulfur compounds could be used to treat inflammation and may be useful in the development of cosmetics. |
| first_indexed | 2024-03-10T11:34:31Z |
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| id | doaj.art-4a87a9d63c3646cc9d39ef92e8dd0350 |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-10T11:34:31Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-4a87a9d63c3646cc9d39ef92e8dd03502023-11-21T18:56:23ZengMDPI AGLife2075-17292021-05-0111542710.3390/life11050427Natural Sulfurs Inhibit LPS-Induced Inflammatory Responses through NF-κB Signaling in CCD-986Sk Skin FibroblastsNipin Sp0Dong Young Kang1Hyoung Do Kim2Alexis Rugamba3Eun Seong Jo4Jong-Chan Park5Se Won Bae6Jin-Moo Lee7Kyoung-Jin Jang8Department of Pathology, School of Medicine, Institute of Biomedical Science and Technology, Konkuk University, Chungju 27478, KoreaDepartment of Pathology, School of Medicine, Institute of Biomedical Science and Technology, Konkuk University, Chungju 27478, KoreaDepartment of Pathology, School of Medicine, Institute of Biomedical Science and Technology, Konkuk University, Chungju 27478, KoreaDepartment of Pathology, School of Medicine, Institute of Biomedical Science and Technology, Konkuk University, Chungju 27478, KoreaPharmacological Research Division, National Institute of Food and Drug Safety Evaluation, Osong Health Technology Administration Complex, Cheongju 28159, KoreaPlant Systems Engineering Research Center, Korea Research Institute of Bioscience & Biotechnology, Daejeon 34141, KoreaDepartment of Chemistry and Cosmetics, Jeju National University, Jeju 63243, KoreaPharmacological Research Division, National Institute of Food and Drug Safety Evaluation, Osong Health Technology Administration Complex, Cheongju 28159, KoreaDepartment of Pathology, School of Medicine, Institute of Biomedical Science and Technology, Konkuk University, Chungju 27478, KoreaLipopolysaccharide (LPS)-induced inflammatory response leads to serious damage, up to and including tumorigenesis. Natural mineral sulfur, non-toxic sulfur (NTS), and methylsulfonylmethane (MSM) have anti-inflammatory activity that may inhibit LPS-induced inflammation. We hypothesized that sulfur compounds could inhibit LPS-induced inflammatory responses in CCD-986Sk skin fibroblasts. We used Western blotting and real-time PCR to analyze molecular signaling in treated and untreated cultures. We also used flow cytometry for cell surface receptor analysis, comet assays to evaluate DNA damage, and ELISA-based cytokine detection. LPS induced TLR4 activation and NF-κB signaling via canonical and protein kinase C (PKC)-dependent pathways, while NTS and MSM downregulated that response. NTS and MSM also inhibited LPS-induced nuclear accumulation and binding of NF-κB to proinflammatory cytokines COX-2, IL-1β, and IL-6. Finally, the sulfur compounds suppressed LPS-induced ROS accumulation and DNA damage in CCD-986Sk cells. These results suggest that natural sulfur compounds could be used to treat inflammation and may be useful in the development of cosmetics.https://www.mdpi.com/2075-1729/11/5/427CCD-986SkLPSNTSMSMTLR4NF-κB |
| spellingShingle | Nipin Sp Dong Young Kang Hyoung Do Kim Alexis Rugamba Eun Seong Jo Jong-Chan Park Se Won Bae Jin-Moo Lee Kyoung-Jin Jang Natural Sulfurs Inhibit LPS-Induced Inflammatory Responses through NF-κB Signaling in CCD-986Sk Skin Fibroblasts Life CCD-986Sk LPS NTS MSM TLR4 NF-κB |
| title | Natural Sulfurs Inhibit LPS-Induced Inflammatory Responses through NF-κB Signaling in CCD-986Sk Skin Fibroblasts |
| title_full | Natural Sulfurs Inhibit LPS-Induced Inflammatory Responses through NF-κB Signaling in CCD-986Sk Skin Fibroblasts |
| title_fullStr | Natural Sulfurs Inhibit LPS-Induced Inflammatory Responses through NF-κB Signaling in CCD-986Sk Skin Fibroblasts |
| title_full_unstemmed | Natural Sulfurs Inhibit LPS-Induced Inflammatory Responses through NF-κB Signaling in CCD-986Sk Skin Fibroblasts |
| title_short | Natural Sulfurs Inhibit LPS-Induced Inflammatory Responses through NF-κB Signaling in CCD-986Sk Skin Fibroblasts |
| title_sort | natural sulfurs inhibit lps induced inflammatory responses through nf κb signaling in ccd 986sk skin fibroblasts |
| topic | CCD-986Sk LPS NTS MSM TLR4 NF-κB |
| url | https://www.mdpi.com/2075-1729/11/5/427 |
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