Preparation of Apoptotic Extracellular Vesicles from Adipose Tissue and Their Efficacy in Promoting High-Quality Skin Wound Healing
Jia Dong,1 Bin Wu,1 Weidong Tian2,3 1Department of Stomatology, People’s Hospital of Longhua Shenzhen, Shenzhen, Guangdong, People’s Republic of China; 2State Key Laboratory of Oral Disease, Engineering Research Center of Oral Translational Medicine, Ministry of Education, West China School of Stoma...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Dove Medical Press
2023-06-01
|
| Series: | International Journal of Nanomedicine |
| Subjects: | |
| Online Access: | https://www.dovepress.com/preparation-of-apoptotic-extracellular-vesicles-from-adipose-tissue-an-peer-reviewed-fulltext-article-IJN |
| _version_ | 1797809971549700096 |
|---|---|
| author | Dong J Wu B Tian W |
| author_facet | Dong J Wu B Tian W |
| author_sort | Dong J |
| collection | DOAJ |
| description | Jia Dong,1 Bin Wu,1 Weidong Tian2,3 1Department of Stomatology, People’s Hospital of Longhua Shenzhen, Shenzhen, Guangdong, People’s Republic of China; 2State Key Laboratory of Oral Disease, Engineering Research Center of Oral Translational Medicine, Ministry of Education, West China School of Stomatology, Sichuan University, Chengdu, Sichuan, People’s Republic of China; 3National Engineering Laboratory for Oral Regenerative Medicine, Sichuan University, Chengdu, Sichuan, People’s Republic of ChinaCorrespondence: Jia Dong; Weidong Tian, Email scu2013dongjia@sina.com; drtwd@sina.comPurpose: A lot of strategies have been attempted to achieve high-quality skin wound healing, among them, fat transplantation has been used for skin wound repair and scar management and has shown beneficial effects. However, the underlying mechanism is still unclear. Recently, studies found that transplanted cells underwent apoptosis within a short period and apoptotic extracellular vesicles (ApoEVs) might play the therapeutic role.Methods: In this study, we directly isolated apoptotic extracellular vesicles from adipose tissue (ApoEVs-AT) and evaluated their characteristics. In vivo, we investigated the therapeutic role of ApoEVs-AT in full-thickness skin wounds. The rate of wound healing, the quality of granulation tissue, and the area of scars were evaluated here. In vitro, we investigated the cellular behaviors of fibroblasts and endothelial cells induced by ApoEVs-AT, including cellular uptake, proliferation, migration, and differentiation.Results: ApoEVs-AT could be successfully isolated from adipose tissue and possessed the basic characteristics of ApoEVs. In vivo, ApoEVs-AT could accelerate skin wound healing, improve the quality of granulation tissue, and reduce the area of scars. In vitro, ApoEVs-AT could be engulfed by fibroblasts and endothelial cells, significantly enhancing their proliferation and migration. Moreover, ApoEVs-AT could promote adipogenic differentiation and inhibit the fibrogenic differentiation of fibroblasts.Conclusion: These findings indicated that ApoEVs could be successfully prepared from adipose tissue and showed the ability to promote high-quality skin wound healing by modulating fibroblasts and endothelial cells.Keywords: apoptotic extracellular vesicles, adipose tissue, skin wound healing, fibroblasts, endothelial cells |
| first_indexed | 2024-03-13T07:01:00Z |
| format | Article |
| id | doaj.art-4a8935b1fb784941b596403ca2330ded |
| institution | Directory Open Access Journal |
| issn | 1178-2013 |
| language | English |
| last_indexed | 2024-03-13T07:01:00Z |
| publishDate | 2023-06-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Nanomedicine |
| spelling | doaj.art-4a8935b1fb784941b596403ca2330ded2023-06-06T19:22:44ZengDove Medical PressInternational Journal of Nanomedicine1178-20132023-06-01Volume 182923293884165Preparation of Apoptotic Extracellular Vesicles from Adipose Tissue and Their Efficacy in Promoting High-Quality Skin Wound HealingDong JWu BTian WJia Dong,1 Bin Wu,1 Weidong Tian2,3 1Department of Stomatology, People’s Hospital of Longhua Shenzhen, Shenzhen, Guangdong, People’s Republic of China; 2State Key Laboratory of Oral Disease, Engineering Research Center of Oral Translational Medicine, Ministry of Education, West China School of Stomatology, Sichuan University, Chengdu, Sichuan, People’s Republic of China; 3National Engineering Laboratory for Oral Regenerative Medicine, Sichuan University, Chengdu, Sichuan, People’s Republic of ChinaCorrespondence: Jia Dong; Weidong Tian, Email scu2013dongjia@sina.com; drtwd@sina.comPurpose: A lot of strategies have been attempted to achieve high-quality skin wound healing, among them, fat transplantation has been used for skin wound repair and scar management and has shown beneficial effects. However, the underlying mechanism is still unclear. Recently, studies found that transplanted cells underwent apoptosis within a short period and apoptotic extracellular vesicles (ApoEVs) might play the therapeutic role.Methods: In this study, we directly isolated apoptotic extracellular vesicles from adipose tissue (ApoEVs-AT) and evaluated their characteristics. In vivo, we investigated the therapeutic role of ApoEVs-AT in full-thickness skin wounds. The rate of wound healing, the quality of granulation tissue, and the area of scars were evaluated here. In vitro, we investigated the cellular behaviors of fibroblasts and endothelial cells induced by ApoEVs-AT, including cellular uptake, proliferation, migration, and differentiation.Results: ApoEVs-AT could be successfully isolated from adipose tissue and possessed the basic characteristics of ApoEVs. In vivo, ApoEVs-AT could accelerate skin wound healing, improve the quality of granulation tissue, and reduce the area of scars. In vitro, ApoEVs-AT could be engulfed by fibroblasts and endothelial cells, significantly enhancing their proliferation and migration. Moreover, ApoEVs-AT could promote adipogenic differentiation and inhibit the fibrogenic differentiation of fibroblasts.Conclusion: These findings indicated that ApoEVs could be successfully prepared from adipose tissue and showed the ability to promote high-quality skin wound healing by modulating fibroblasts and endothelial cells.Keywords: apoptotic extracellular vesicles, adipose tissue, skin wound healing, fibroblasts, endothelial cellshttps://www.dovepress.com/preparation-of-apoptotic-extracellular-vesicles-from-adipose-tissue-an-peer-reviewed-fulltext-article-IJNapoptotic extracellular vesiclesadipose tissueskin wound healingfibroblastsendothelial cells |
| spellingShingle | Dong J Wu B Tian W Preparation of Apoptotic Extracellular Vesicles from Adipose Tissue and Their Efficacy in Promoting High-Quality Skin Wound Healing International Journal of Nanomedicine apoptotic extracellular vesicles adipose tissue skin wound healing fibroblasts endothelial cells |
| title | Preparation of Apoptotic Extracellular Vesicles from Adipose Tissue and Their Efficacy in Promoting High-Quality Skin Wound Healing |
| title_full | Preparation of Apoptotic Extracellular Vesicles from Adipose Tissue and Their Efficacy in Promoting High-Quality Skin Wound Healing |
| title_fullStr | Preparation of Apoptotic Extracellular Vesicles from Adipose Tissue and Their Efficacy in Promoting High-Quality Skin Wound Healing |
| title_full_unstemmed | Preparation of Apoptotic Extracellular Vesicles from Adipose Tissue and Their Efficacy in Promoting High-Quality Skin Wound Healing |
| title_short | Preparation of Apoptotic Extracellular Vesicles from Adipose Tissue and Their Efficacy in Promoting High-Quality Skin Wound Healing |
| title_sort | preparation of apoptotic extracellular vesicles from adipose tissue and their efficacy in promoting high quality skin wound healing |
| topic | apoptotic extracellular vesicles adipose tissue skin wound healing fibroblasts endothelial cells |
| url | https://www.dovepress.com/preparation-of-apoptotic-extracellular-vesicles-from-adipose-tissue-an-peer-reviewed-fulltext-article-IJN |
| work_keys_str_mv | AT dongj preparationofapoptoticextracellularvesiclesfromadiposetissueandtheirefficacyinpromotinghighqualityskinwoundhealing AT wub preparationofapoptoticextracellularvesiclesfromadiposetissueandtheirefficacyinpromotinghighqualityskinwoundhealing AT tianw preparationofapoptoticextracellularvesiclesfromadiposetissueandtheirefficacyinpromotinghighqualityskinwoundhealing |