Calbindin D28k-Immunoreactivity in Human Enteric Neurons
Calbindin (CALB) is well established as immunohistochemical marker for intrinsic primary afferent neurons in the guinea pig gut. Its expression by numerous human enteric neurons has been demonstrated but little is known about particular types of neurons immunoreactive for CALB. Here we investigated...
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MDPI AG
2018-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | http://www.mdpi.com/1422-0067/19/1/194 |
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| author | Katharina Zetzmann Johanna Strehl Carol Geppert Stefanie Kuerten Samir Jabari Axel Brehmer |
| author_facet | Katharina Zetzmann Johanna Strehl Carol Geppert Stefanie Kuerten Samir Jabari Axel Brehmer |
| author_sort | Katharina Zetzmann |
| collection | DOAJ |
| description | Calbindin (CALB) is well established as immunohistochemical marker for intrinsic primary afferent neurons in the guinea pig gut. Its expression by numerous human enteric neurons has been demonstrated but little is known about particular types of neurons immunoreactive for CALB. Here we investigated small and large intestinal wholemount sets of 26 tumor patients in order to evaluate (1) the proportion of CALB+ neurons in the total neuron population, (2) the colocalization of CALB with calretinin (CALR), somatostatin (SOM) and vasoactive intestinal peptide (VIP) and (3) the morphology of CALB+ neurons. CALB+ neurons represented a minority of myenteric neurons (small intestine: 31%; large intestine: 25%) and the majority of submucosal neurons (between 72 and 95%). In the submucosa, most CALB+ neurons co-stained for CALR and VIP (between 69 and 80%) or for SOM (between 20 and 3%). In the myenteric plexus, 85% of CALB+ neurons did not co-stain with the other markers investigated. An unequivocal correlation between CALB reactivity and neuronal morphology was found for myenteric type III neurons in the small intestine: uniaxonal neurons with long, slender and branched dendrites were generally positive for CALB. Since also other neurons displayed occasional CALB reactivity, this protein is not suited as an exclusive marker for type III neurons. |
| first_indexed | 2024-04-12T10:48:53Z |
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| issn | 1422-0067 |
| language | English |
| last_indexed | 2024-04-12T10:48:53Z |
| publishDate | 2018-01-01 |
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| spelling | doaj.art-4a8c93676c164ad486526c830c791e712022-12-22T03:36:19ZengMDPI AGInternational Journal of Molecular Sciences1422-00672018-01-0119119410.3390/ijms19010194ijms19010194Calbindin D28k-Immunoreactivity in Human Enteric NeuronsKatharina Zetzmann0Johanna Strehl1Carol Geppert2Stefanie Kuerten3Samir Jabari4Axel Brehmer5Institute of Anatomy and Cell Biology, University of Erlangen-Nuremberg, Krankenhausstraße 9, D-91054 Erlangen, GermanyInstitute of Pathology, University of Erlangen-Nuremberg, Krankenhausstraße 8-10, D-91054 Erlangen, GermanyInstitute of Pathology, University of Erlangen-Nuremberg, Krankenhausstraße 8-10, D-91054 Erlangen, GermanyInstitute of Anatomy and Cell Biology, University of Erlangen-Nuremberg, Krankenhausstraße 9, D-91054 Erlangen, GermanyInstitute of Anatomy and Cell Biology, University of Erlangen-Nuremberg, Krankenhausstraße 9, D-91054 Erlangen, GermanyInstitute of Anatomy and Cell Biology, University of Erlangen-Nuremberg, Krankenhausstraße 9, D-91054 Erlangen, GermanyCalbindin (CALB) is well established as immunohistochemical marker for intrinsic primary afferent neurons in the guinea pig gut. Its expression by numerous human enteric neurons has been demonstrated but little is known about particular types of neurons immunoreactive for CALB. Here we investigated small and large intestinal wholemount sets of 26 tumor patients in order to evaluate (1) the proportion of CALB+ neurons in the total neuron population, (2) the colocalization of CALB with calretinin (CALR), somatostatin (SOM) and vasoactive intestinal peptide (VIP) and (3) the morphology of CALB+ neurons. CALB+ neurons represented a minority of myenteric neurons (small intestine: 31%; large intestine: 25%) and the majority of submucosal neurons (between 72 and 95%). In the submucosa, most CALB+ neurons co-stained for CALR and VIP (between 69 and 80%) or for SOM (between 20 and 3%). In the myenteric plexus, 85% of CALB+ neurons did not co-stain with the other markers investigated. An unequivocal correlation between CALB reactivity and neuronal morphology was found for myenteric type III neurons in the small intestine: uniaxonal neurons with long, slender and branched dendrites were generally positive for CALB. Since also other neurons displayed occasional CALB reactivity, this protein is not suited as an exclusive marker for type III neurons.http://www.mdpi.com/1422-0067/19/1/194calcium binding proteincalretininenteric nervous systemmorphologymyenteric plexussubmucosal plexus |
| spellingShingle | Katharina Zetzmann Johanna Strehl Carol Geppert Stefanie Kuerten Samir Jabari Axel Brehmer Calbindin D28k-Immunoreactivity in Human Enteric Neurons International Journal of Molecular Sciences calcium binding protein calretinin enteric nervous system morphology myenteric plexus submucosal plexus |
| title | Calbindin D28k-Immunoreactivity in Human Enteric Neurons |
| title_full | Calbindin D28k-Immunoreactivity in Human Enteric Neurons |
| title_fullStr | Calbindin D28k-Immunoreactivity in Human Enteric Neurons |
| title_full_unstemmed | Calbindin D28k-Immunoreactivity in Human Enteric Neurons |
| title_short | Calbindin D28k-Immunoreactivity in Human Enteric Neurons |
| title_sort | calbindin d28k immunoreactivity in human enteric neurons |
| topic | calcium binding protein calretinin enteric nervous system morphology myenteric plexus submucosal plexus |
| url | http://www.mdpi.com/1422-0067/19/1/194 |
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