Multidimensional fluorescence microscopy of multiple organelles in Arabidopsis seedlings
<p>Abstract</p> <p>Background</p> <p>The isolation of green fluorescent protein (GFP) and the development of spectral variants over the past decade have begun to reveal the dynamic nature of protein trafficking and organelle motility. <it>In planta </it>anal...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2008-05-01
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| Series: | Plant Methods |
| Online Access: | http://www.plantmethods.com/content/4/1/9 |
| _version_ | 1811252630774611968 |
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| author | Morales Andrea Fujikawa Yukichi Boisdore Marietta Brown Matthew L Reynolds Dexter Kato Naohiro Meisel Lee A |
| author_facet | Morales Andrea Fujikawa Yukichi Boisdore Marietta Brown Matthew L Reynolds Dexter Kato Naohiro Meisel Lee A |
| author_sort | Morales Andrea |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The isolation of green fluorescent protein (GFP) and the development of spectral variants over the past decade have begun to reveal the dynamic nature of protein trafficking and organelle motility. <it>In planta </it>analyses of this dynamic process have typically been limited to only two organelles or proteins at a time in only a few cell types.</p> <p>Results</p> <p>We generated a transgenic Arabidopsis plant that contains four spectrally different fluorescent proteins. Nuclei, plastids, mitochondria and plasma membranes were genetically tagged with cyan, red, yellow and green fluorescent proteins, respectively. In addition, methods to track nuclei, mitochondria and chloroplasts and quantify the interaction between these organelles at a submicron resolution were developed. These analyzes revealed that N-ethylmaleimide disrupts nuclear-mitochondrial but not nuclear-plastids interactions in root epidermal cells of live Arabidopsis seedlings.</p> <p>Conclusion</p> <p>We developed a tool and associated methods for analyzing the complex dynamic of organelle-organelle interactions in real time <it>in planta</it>. Homozygous transgenic Arabidopsis (Kaleidocell) is available through Arabidopsis Biological Resource Center.</p> |
| first_indexed | 2024-04-12T16:37:30Z |
| format | Article |
| id | doaj.art-4a90100515114432a8f0ad6f19a0ac9e |
| institution | Directory Open Access Journal |
| issn | 1746-4811 |
| language | English |
| last_indexed | 2024-04-12T16:37:30Z |
| publishDate | 2008-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Plant Methods |
| spelling | doaj.art-4a90100515114432a8f0ad6f19a0ac9e2022-12-22T03:24:55ZengBMCPlant Methods1746-48112008-05-0141910.1186/1746-4811-4-9Multidimensional fluorescence microscopy of multiple organelles in Arabidopsis seedlingsMorales AndreaFujikawa YukichiBoisdore MariettaBrown Matthew LReynolds DexterKato NaohiroMeisel Lee A<p>Abstract</p> <p>Background</p> <p>The isolation of green fluorescent protein (GFP) and the development of spectral variants over the past decade have begun to reveal the dynamic nature of protein trafficking and organelle motility. <it>In planta </it>analyses of this dynamic process have typically been limited to only two organelles or proteins at a time in only a few cell types.</p> <p>Results</p> <p>We generated a transgenic Arabidopsis plant that contains four spectrally different fluorescent proteins. Nuclei, plastids, mitochondria and plasma membranes were genetically tagged with cyan, red, yellow and green fluorescent proteins, respectively. In addition, methods to track nuclei, mitochondria and chloroplasts and quantify the interaction between these organelles at a submicron resolution were developed. These analyzes revealed that N-ethylmaleimide disrupts nuclear-mitochondrial but not nuclear-plastids interactions in root epidermal cells of live Arabidopsis seedlings.</p> <p>Conclusion</p> <p>We developed a tool and associated methods for analyzing the complex dynamic of organelle-organelle interactions in real time <it>in planta</it>. Homozygous transgenic Arabidopsis (Kaleidocell) is available through Arabidopsis Biological Resource Center.</p>http://www.plantmethods.com/content/4/1/9 |
| spellingShingle | Morales Andrea Fujikawa Yukichi Boisdore Marietta Brown Matthew L Reynolds Dexter Kato Naohiro Meisel Lee A Multidimensional fluorescence microscopy of multiple organelles in Arabidopsis seedlings Plant Methods |
| title | Multidimensional fluorescence microscopy of multiple organelles in Arabidopsis seedlings |
| title_full | Multidimensional fluorescence microscopy of multiple organelles in Arabidopsis seedlings |
| title_fullStr | Multidimensional fluorescence microscopy of multiple organelles in Arabidopsis seedlings |
| title_full_unstemmed | Multidimensional fluorescence microscopy of multiple organelles in Arabidopsis seedlings |
| title_short | Multidimensional fluorescence microscopy of multiple organelles in Arabidopsis seedlings |
| title_sort | multidimensional fluorescence microscopy of multiple organelles in arabidopsis seedlings |
| url | http://www.plantmethods.com/content/4/1/9 |
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