Generation of two induced pluripotent stem cell lines and the corresponding isogenic controls from Parkinson’s disease patients carrying the heterozygous mutations c.815G > A (p.R272Q) or c.1348C > T (p.R450C) in the RHOT1 gene encoding Miro1
Fibroblasts from two Parkinson’s disease (PD) patients carrying either the heterozygous mutation c.815G > A (Miro1 p.R272Q) or c.1348C > T (Miro1 p.R450C) in the RHOT1 gene, were converted into induced pluripotent stem cells (iPSCs) using RNA-based and episomal reprogramming, respectively. The...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-09-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506123001319 |
| _version_ | 1797692045230342144 |
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| author | Axel Chemla Giuseppe Arena Gizem Onal Jonas Walter Clara Berenguer-Escuder Dajana Grossmann Anne Grünewald Jens C. Schwamborn Rejko Krüger |
| author_facet | Axel Chemla Giuseppe Arena Gizem Onal Jonas Walter Clara Berenguer-Escuder Dajana Grossmann Anne Grünewald Jens C. Schwamborn Rejko Krüger |
| author_sort | Axel Chemla |
| collection | DOAJ |
| description | Fibroblasts from two Parkinson’s disease (PD) patients carrying either the heterozygous mutation c.815G > A (Miro1 p.R272Q) or c.1348C > T (Miro1 p.R450C) in the RHOT1 gene, were converted into induced pluripotent stem cells (iPSCs) using RNA-based and episomal reprogramming, respectively. The corresponding isogenic gene-corrected lines have been generated using CRISPR/Cas9 technology. These two isogenic pairs will be used to study Miro1-related molecular mechanisms underlying neurodegeneration in relevant iPSC-derived neuronal models (e.g., midbrain dopaminergic neurons and astrocytes). |
| first_indexed | 2024-03-12T02:21:59Z |
| format | Article |
| id | doaj.art-4a91b68674934c279644a2bb4ca1375a |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-03-12T02:21:59Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-4a91b68674934c279644a2bb4ca1375a2023-09-06T04:50:45ZengElsevierStem Cell Research1873-50612023-09-0171103145Generation of two induced pluripotent stem cell lines and the corresponding isogenic controls from Parkinson’s disease patients carrying the heterozygous mutations c.815G > A (p.R272Q) or c.1348C > T (p.R450C) in the RHOT1 gene encoding Miro1Axel Chemla0Giuseppe Arena1Gizem Onal2Jonas Walter3Clara Berenguer-Escuder4Dajana Grossmann5Anne Grünewald6Jens C. Schwamborn7Rejko Krüger8Translational Neuroscience, Luxembourg Centre for Systems Biomedicine (LCSB), University of Luxembourg, LuxembourgTranslational Neuroscience, Luxembourg Centre for Systems Biomedicine (LCSB), University of Luxembourg, Luxembourg; Corresponding authors at: Translational Neuroscience team, Luxembourg Centre for Systems Biomedicine (LCSB), University of Luxembourg, Campus Belval, 6 Avenue du Swing, L-4367 Esch-sur-Alzette, Luxembourg.Department of Physiology, Anatomy and Genetics, University of Oxford, UK; Kavli Institute for Nanoscience Discovery, University of Oxford, UK; Department of Medical Biology, Faculty of Medicine, Balikesir University, TurkeyDevelopmental and Cellular Biology, Luxembourg Centre for Systems Biomedicine (LCSB), University of Luxembourg, LuxembourgTranslational Neuroscience, Luxembourg Centre for Systems Biomedicine (LCSB), University of Luxembourg, LuxembourgTranslational Neuroscience, Luxembourg Centre for Systems Biomedicine (LCSB), University of Luxembourg, Luxembourg; Translational Neurodegeneration Section “Albrecht-Kossel”, Department of Neurology, University Medical Center Rostock, University of Rostock, Rostock, GermanyMolecular and Functional Neurobiology, Luxembourg Centre for Systems Biomedicine (LCSB), University of Luxembourg, Luxembourg; Institute of Neurogenetics, University of Lübeck, Lübeck, GermanyDevelopmental and Cellular Biology, Luxembourg Centre for Systems Biomedicine (LCSB), University of Luxembourg, LuxembourgTranslational Neuroscience, Luxembourg Centre for Systems Biomedicine (LCSB), University of Luxembourg, Luxembourg; Centre Hospitalier de Luxembourg, Luxembourg; Transversal Translational Medicine, Luxembourg Institute of Health (LIH), Luxembourg; Corresponding authors at: Translational Neuroscience team, Luxembourg Centre for Systems Biomedicine (LCSB), University of Luxembourg, Campus Belval, 6 Avenue du Swing, L-4367 Esch-sur-Alzette, Luxembourg.Fibroblasts from two Parkinson’s disease (PD) patients carrying either the heterozygous mutation c.815G > A (Miro1 p.R272Q) or c.1348C > T (Miro1 p.R450C) in the RHOT1 gene, were converted into induced pluripotent stem cells (iPSCs) using RNA-based and episomal reprogramming, respectively. The corresponding isogenic gene-corrected lines have been generated using CRISPR/Cas9 technology. These two isogenic pairs will be used to study Miro1-related molecular mechanisms underlying neurodegeneration in relevant iPSC-derived neuronal models (e.g., midbrain dopaminergic neurons and astrocytes).http://www.sciencedirect.com/science/article/pii/S1873506123001319 |
| spellingShingle | Axel Chemla Giuseppe Arena Gizem Onal Jonas Walter Clara Berenguer-Escuder Dajana Grossmann Anne Grünewald Jens C. Schwamborn Rejko Krüger Generation of two induced pluripotent stem cell lines and the corresponding isogenic controls from Parkinson’s disease patients carrying the heterozygous mutations c.815G > A (p.R272Q) or c.1348C > T (p.R450C) in the RHOT1 gene encoding Miro1 Stem Cell Research |
| title | Generation of two induced pluripotent stem cell lines and the corresponding isogenic controls from Parkinson’s disease patients carrying the heterozygous mutations c.815G > A (p.R272Q) or c.1348C > T (p.R450C) in the RHOT1 gene encoding Miro1 |
| title_full | Generation of two induced pluripotent stem cell lines and the corresponding isogenic controls from Parkinson’s disease patients carrying the heterozygous mutations c.815G > A (p.R272Q) or c.1348C > T (p.R450C) in the RHOT1 gene encoding Miro1 |
| title_fullStr | Generation of two induced pluripotent stem cell lines and the corresponding isogenic controls from Parkinson’s disease patients carrying the heterozygous mutations c.815G > A (p.R272Q) or c.1348C > T (p.R450C) in the RHOT1 gene encoding Miro1 |
| title_full_unstemmed | Generation of two induced pluripotent stem cell lines and the corresponding isogenic controls from Parkinson’s disease patients carrying the heterozygous mutations c.815G > A (p.R272Q) or c.1348C > T (p.R450C) in the RHOT1 gene encoding Miro1 |
| title_short | Generation of two induced pluripotent stem cell lines and the corresponding isogenic controls from Parkinson’s disease patients carrying the heterozygous mutations c.815G > A (p.R272Q) or c.1348C > T (p.R450C) in the RHOT1 gene encoding Miro1 |
| title_sort | generation of two induced pluripotent stem cell lines and the corresponding isogenic controls from parkinson s disease patients carrying the heterozygous mutations c 815g a p r272q or c 1348c t p r450c in the rhot1 gene encoding miro1 |
| url | http://www.sciencedirect.com/science/article/pii/S1873506123001319 |
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