Integrative analysis of proteomics and lipidomic profiles reveal the fat deposition and meat quality in Duroc × Guangdong small spotted pig
IntroductionThis study aims to explore the important factors affecting the characteristics of different parts of pork.MethodsLipidomics and proteomics methods were used to analyze DAL (differential lipids) and DAPs (differential proteins) in five different parts (longissimus dorsi, belly meat, loin,...
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Frontiers Media S.A.
2024-04-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2024.1361441/full |
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| author | Zhuosui Wu Zhonggang Wang Pan Wang Leiyan Cheng Jianhao Li Yanfeng Luo Linfang Yang Linfeng Li Jianhua Zeng Bin Hu |
| author_facet | Zhuosui Wu Zhonggang Wang Pan Wang Leiyan Cheng Jianhao Li Yanfeng Luo Linfang Yang Linfeng Li Jianhua Zeng Bin Hu |
| author_sort | Zhuosui Wu |
| collection | DOAJ |
| description | IntroductionThis study aims to explore the important factors affecting the characteristics of different parts of pork.MethodsLipidomics and proteomics methods were used to analyze DAL (differential lipids) and DAPs (differential proteins) in five different parts (longissimus dorsi, belly meat, loin, forelegs and buttocks) of Duhua pig (Duroc × Guangdong small spotted pig), to identify potential pathways affecting meat quality, investigating fat deposition in pork and its lipid-protein interactions.ResultsThe results show that TG (triglyceride) is the lipid subclass with the highest proportion in muscle, and the pathway with the most significantly enriched lipids is GP. DAP clustered on several GO terms closely related to lipid metabolism and lipogenesis (lipid binding, lipid metabolism, lipid transport, and lipid regulation). In KEGG analysis, there are two main DAP aggregation pathways related to lipid metabolism, namely Fatty acid degradation and oxidative phosphorylation. In PPI analysis, we screened out 31 core proteins, among which NDUFA6, NDUFA9 and ACO2 are the most critical.DiscussionPC (phosphatidylcholine) is regulated by SNX5, THBS1, ANXA7, TPP1, CAVIN2, and VDAC2 in the phospholipid binding pathway. TG is regulated by AUH/HADH/ACADM/ACADL/HADHA in the lipid oxidation and lipid modification pathways. Potential biomarkers are rich in SFA, MUFA and PUFA respectively, the amounts of SFA, MUFA and PUFA in the lipid measurement results are consistent with the up- and down-regulation of potential biomarker lipids. This study clarified the differences in protein and lipid compositions in different parts of Duhua pigs and provided data support for revealing the interactions between pork lipids and proteins. These findings provide contributions to the study of intramuscular fat deposition in pork from a genetic and nutritional perspective. |
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| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-04-24T11:26:36Z |
| publishDate | 2024-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-4a92b4cd6950499d871b65ed29571d4c2024-04-10T13:26:57ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692024-04-011110.3389/fvets.2024.13614411361441Integrative analysis of proteomics and lipidomic profiles reveal the fat deposition and meat quality in Duroc × Guangdong small spotted pigZhuosui Wu0Zhonggang Wang1Pan Wang2Leiyan Cheng3Jianhao Li4Yanfeng Luo5Linfang Yang6Linfeng Li7Jianhua Zeng8Bin Hu9Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou, ChinaGuangdong Guanghong Agriculture and Animal Husbandry Development Co, Ltd., Huizhou, ChinaGuangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou, ChinaGuangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou, ChinaGuangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou, ChinaGuangdong Yihao Foodstuff Co, Ltd., Guangzhou, ChinaGuangdong Yihao Foodstuff Co, Ltd., Guangzhou, ChinaGuangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou, ChinaGuangdong Yihao Foodstuff Co, Ltd., Guangzhou, ChinaGuangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou, ChinaIntroductionThis study aims to explore the important factors affecting the characteristics of different parts of pork.MethodsLipidomics and proteomics methods were used to analyze DAL (differential lipids) and DAPs (differential proteins) in five different parts (longissimus dorsi, belly meat, loin, forelegs and buttocks) of Duhua pig (Duroc × Guangdong small spotted pig), to identify potential pathways affecting meat quality, investigating fat deposition in pork and its lipid-protein interactions.ResultsThe results show that TG (triglyceride) is the lipid subclass with the highest proportion in muscle, and the pathway with the most significantly enriched lipids is GP. DAP clustered on several GO terms closely related to lipid metabolism and lipogenesis (lipid binding, lipid metabolism, lipid transport, and lipid regulation). In KEGG analysis, there are two main DAP aggregation pathways related to lipid metabolism, namely Fatty acid degradation and oxidative phosphorylation. In PPI analysis, we screened out 31 core proteins, among which NDUFA6, NDUFA9 and ACO2 are the most critical.DiscussionPC (phosphatidylcholine) is regulated by SNX5, THBS1, ANXA7, TPP1, CAVIN2, and VDAC2 in the phospholipid binding pathway. TG is regulated by AUH/HADH/ACADM/ACADL/HADHA in the lipid oxidation and lipid modification pathways. Potential biomarkers are rich in SFA, MUFA and PUFA respectively, the amounts of SFA, MUFA and PUFA in the lipid measurement results are consistent with the up- and down-regulation of potential biomarker lipids. This study clarified the differences in protein and lipid compositions in different parts of Duhua pigs and provided data support for revealing the interactions between pork lipids and proteins. These findings provide contributions to the study of intramuscular fat deposition in pork from a genetic and nutritional perspective.https://www.frontiersin.org/articles/10.3389/fvets.2024.1361441/fullfat depositionlipidomicsproteomicsmetabolic pathwaysDuroc × Guangdong small spotted pig |
| spellingShingle | Zhuosui Wu Zhonggang Wang Pan Wang Leiyan Cheng Jianhao Li Yanfeng Luo Linfang Yang Linfeng Li Jianhua Zeng Bin Hu Integrative analysis of proteomics and lipidomic profiles reveal the fat deposition and meat quality in Duroc × Guangdong small spotted pig Frontiers in Veterinary Science fat deposition lipidomics proteomics metabolic pathways Duroc × Guangdong small spotted pig |
| title | Integrative analysis of proteomics and lipidomic profiles reveal the fat deposition and meat quality in Duroc × Guangdong small spotted pig |
| title_full | Integrative analysis of proteomics and lipidomic profiles reveal the fat deposition and meat quality in Duroc × Guangdong small spotted pig |
| title_fullStr | Integrative analysis of proteomics and lipidomic profiles reveal the fat deposition and meat quality in Duroc × Guangdong small spotted pig |
| title_full_unstemmed | Integrative analysis of proteomics and lipidomic profiles reveal the fat deposition and meat quality in Duroc × Guangdong small spotted pig |
| title_short | Integrative analysis of proteomics and lipidomic profiles reveal the fat deposition and meat quality in Duroc × Guangdong small spotted pig |
| title_sort | integrative analysis of proteomics and lipidomic profiles reveal the fat deposition and meat quality in duroc guangdong small spotted pig |
| topic | fat deposition lipidomics proteomics metabolic pathways Duroc × Guangdong small spotted pig |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2024.1361441/full |
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