Postoperative Complications Are Associated with Long-Term Changes in the Gut Microbiota Following Colorectal Cancer Surgery
Changes in the gut microbiome have already been associated with postoperative complications in major abdominal surgery. However, it is still unclear whether these changes are transient or a long-lasting effect. Therefore, the aim of this prospective clinical pilot study was to examine long-term chan...
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MDPI AG
2021-03-01
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| Online Access: | https://www.mdpi.com/2075-1729/11/3/246 |
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| author | Felix C. F. Schmitt Martin Schneider William Mathejczyk Markus A. Weigand Jane C. Figueiredo Christopher I. Li David Shibata Erin M. Siegel Adetunji T. Toriola Cornelia M. Ulrich Alexis B. Ulrich Sébastien Boutin Biljana Gigic |
| author_facet | Felix C. F. Schmitt Martin Schneider William Mathejczyk Markus A. Weigand Jane C. Figueiredo Christopher I. Li David Shibata Erin M. Siegel Adetunji T. Toriola Cornelia M. Ulrich Alexis B. Ulrich Sébastien Boutin Biljana Gigic |
| author_sort | Felix C. F. Schmitt |
| collection | DOAJ |
| description | Changes in the gut microbiome have already been associated with postoperative complications in major abdominal surgery. However, it is still unclear whether these changes are transient or a long-lasting effect. Therefore, the aim of this prospective clinical pilot study was to examine long-term changes in the gut microbiota and to correlate these changes with the clinical course of the patient. Methods: In total, stool samples of 62 newly diagnosed colorectal cancer patients undergoing primary tumor resection were analyzed by 16S-rDNA next-generation sequencing. Stool samples were collected preoperatively in order to determine the gut microbiome at baseline as well as at 6, 12, and 24 months thereafter to observe longitudinal changes. Postoperatively, the study patients were separated into two groups—patients who suffered from postoperative complications (<i>n</i> = 30) and those without complication (<i>n</i> = 32). Patients with postoperative complications showed a significantly stronger reduction in the alpha diversity starting 6 months after operation, which does not resolve, even after 24 months. The structure of the microbiome was also significantly altered from baseline at six-month follow-up in patients with complications (<i>p</i> = 0.006). This was associated with a long-lasting decrease of a large number of species in the gut microbiota indicating an impact in the commensal microbiota and a long-lasting increase of <i>Fusobacterium ulcerans</i>. The microbial composition of the gut microbiome shows significant changes in patients with postoperative complications up to 24 months after surgery. |
| first_indexed | 2024-03-10T13:10:50Z |
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| language | English |
| last_indexed | 2024-03-10T13:10:50Z |
| publishDate | 2021-03-01 |
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| series | Life |
| spelling | doaj.art-4a93a608d0704b9b8f8b64db48b46ea22023-11-21T10:45:29ZengMDPI AGLife2075-17292021-03-0111324610.3390/life11030246Postoperative Complications Are Associated with Long-Term Changes in the Gut Microbiota Following Colorectal Cancer SurgeryFelix C. F. Schmitt0Martin Schneider1William Mathejczyk2Markus A. Weigand3Jane C. Figueiredo4Christopher I. Li5David Shibata6Erin M. Siegel7Adetunji T. Toriola8Cornelia M. Ulrich9Alexis B. Ulrich10Sébastien Boutin11Biljana Gigic12Department of Anesthesiology, Heidelberg University Hospital, 69120 Heidelberg, GermanyDepartment of General, Visceral and Transplant Surgery, Heidelberg University Hospital, 69120 Heidelberg, GermanyDepartment of Anesthesiology, Heidelberg University Hospital, 69120 Heidelberg, GermanyDepartment of Anesthesiology, Heidelberg University Hospital, 69120 Heidelberg, GermanyDepartment of Medicine, Samuel Oschin Comprehensive Cancer Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USAPublic Health Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USADepartment of Surgery, University of Tennessee Health Science Center, Memphis, TN 38163, USACancer Epidemiology Program, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL 33612, USADivision of Public Health Sciences, Department of Surgery, Washington University School of Medicine and Siteman Cancer Center, St Louis, MI 63110, USAPopulation Sciences, Huntsman Cancer Institute, Salt Lake City, UT 84112, USADepartment of General, Visceral and Transplant Surgery, Heidelberg University Hospital, 69120 Heidelberg, GermanyDepartment of Infectious Diseases, Medical Microbiology and Hygiene, Heidelberg University Hospital, 69120 Heidelberg, GermanyDepartment of General, Visceral and Transplant Surgery, Heidelberg University Hospital, 69120 Heidelberg, GermanyChanges in the gut microbiome have already been associated with postoperative complications in major abdominal surgery. However, it is still unclear whether these changes are transient or a long-lasting effect. Therefore, the aim of this prospective clinical pilot study was to examine long-term changes in the gut microbiota and to correlate these changes with the clinical course of the patient. Methods: In total, stool samples of 62 newly diagnosed colorectal cancer patients undergoing primary tumor resection were analyzed by 16S-rDNA next-generation sequencing. Stool samples were collected preoperatively in order to determine the gut microbiome at baseline as well as at 6, 12, and 24 months thereafter to observe longitudinal changes. Postoperatively, the study patients were separated into two groups—patients who suffered from postoperative complications (<i>n</i> = 30) and those without complication (<i>n</i> = 32). Patients with postoperative complications showed a significantly stronger reduction in the alpha diversity starting 6 months after operation, which does not resolve, even after 24 months. The structure of the microbiome was also significantly altered from baseline at six-month follow-up in patients with complications (<i>p</i> = 0.006). This was associated with a long-lasting decrease of a large number of species in the gut microbiota indicating an impact in the commensal microbiota and a long-lasting increase of <i>Fusobacterium ulcerans</i>. The microbial composition of the gut microbiome shows significant changes in patients with postoperative complications up to 24 months after surgery.https://www.mdpi.com/2075-1729/11/3/246colorectal surgerypostoperative complicationsgut microbiota16S rDNA gene sequencinginflammationsepsis |
| spellingShingle | Felix C. F. Schmitt Martin Schneider William Mathejczyk Markus A. Weigand Jane C. Figueiredo Christopher I. Li David Shibata Erin M. Siegel Adetunji T. Toriola Cornelia M. Ulrich Alexis B. Ulrich Sébastien Boutin Biljana Gigic Postoperative Complications Are Associated with Long-Term Changes in the Gut Microbiota Following Colorectal Cancer Surgery Life colorectal surgery postoperative complications gut microbiota 16S rDNA gene sequencing inflammation sepsis |
| title | Postoperative Complications Are Associated with Long-Term Changes in the Gut Microbiota Following Colorectal Cancer Surgery |
| title_full | Postoperative Complications Are Associated with Long-Term Changes in the Gut Microbiota Following Colorectal Cancer Surgery |
| title_fullStr | Postoperative Complications Are Associated with Long-Term Changes in the Gut Microbiota Following Colorectal Cancer Surgery |
| title_full_unstemmed | Postoperative Complications Are Associated with Long-Term Changes in the Gut Microbiota Following Colorectal Cancer Surgery |
| title_short | Postoperative Complications Are Associated with Long-Term Changes in the Gut Microbiota Following Colorectal Cancer Surgery |
| title_sort | postoperative complications are associated with long term changes in the gut microbiota following colorectal cancer surgery |
| topic | colorectal surgery postoperative complications gut microbiota 16S rDNA gene sequencing inflammation sepsis |
| url | https://www.mdpi.com/2075-1729/11/3/246 |
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