Oleic acid-induced steatosis model establishment in LMH cells and its effect on lipid metabolism
Hepatic steatosis is a highly prevalent liver disease, yet research on it is hampered by the lack of tractable cellular models in poultry. To examine the possibility of using organoids to model steatosis and detect it efficiently in leghorn male hepatocellular (LMH) cells, we first established steat...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-01-01
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| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579122005922 |
| _version_ | 1811212041581494272 |
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| author | Huiqi Song Ruizhi Yang Jiahao Zhang Pengliang Sun Xiaoyue Xing Lan Wang Ta Sairijima Yahui Hu Yang Liu Huixu Cheng Qiulin Zhang Lianrui Li |
| author_facet | Huiqi Song Ruizhi Yang Jiahao Zhang Pengliang Sun Xiaoyue Xing Lan Wang Ta Sairijima Yahui Hu Yang Liu Huixu Cheng Qiulin Zhang Lianrui Li |
| author_sort | Huiqi Song |
| collection | DOAJ |
| description | Hepatic steatosis is a highly prevalent liver disease, yet research on it is hampered by the lack of tractable cellular models in poultry. To examine the possibility of using organoids to model steatosis and detect it efficiently in leghorn male hepatocellular (LMH) cells, we first established steatosis using different concentrations of oleic acid (OA) (0.05–0.75 mmol/L) for 12 or 24 h. The subsequent detections found that the treatment of LMH cells with OA resulted in a dramatic increase in intracellular triglyceride (TG) concentrations, which was positively associated with the concentration of the inducing OA (R2 > 0.9). Then, the modeled steatosis was detected by flow cytometry after NileRed staining and it was found that the intensity of NileRed-A was positively correlated with the TG concentration (R2 > 0.93), which demonstrates that the flow cytometry is suitable for the detection of steatosis in LMH cells. According to the detection results of the different steatosis models, we confirmed that the optimal induction condition for the establishment of the steatosis model in LMH cells is OA (0.375 mmol/L) incubation for 12 h. Finally, the transcription and protein content of fat metabolism-related genes in steatosis model cells were detected. It was found that OA-induced steatosis could significantly decrease the expression of nuclear receptor PPAR-γ and the synthesis of fatty acids (SREBP-1C, ACC1, FASN), increasing the oxidative decomposition of triglycerides (CPT1A) and the assembly of low-density lipoproteins (MTTP, ApoB). Sterol metabolism in model cells was also significantly enhanced (HMGR, ABCA1, L-BABP). This study established, detected, and analyzed an OA-induced steatosis model in LMH cells, which provides a stable model and detection method for the study of poultry steatosis-related diseases. |
| first_indexed | 2024-04-12T05:22:24Z |
| format | Article |
| id | doaj.art-4a97d83bf49042dca1a77341409fe5c5 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-04-12T05:22:24Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-4a97d83bf49042dca1a77341409fe5c52022-12-22T03:46:24ZengElsevierPoultry Science0032-57912023-01-011021102297Oleic acid-induced steatosis model establishment in LMH cells and its effect on lipid metabolismHuiqi Song0Ruizhi Yang1Jiahao Zhang2Pengliang Sun3Xiaoyue Xing4Lan Wang5Ta Sairijima6Yahui Hu7Yang Liu8Huixu Cheng9Qiulin Zhang10Lianrui Li11College of life science and technology, Tarim University, Alar 843300, Xinjiang, China; Key Laboratory of Tarim Animal Husbandry Science and Technology, Xinjiang Production and Construction Corps, Alar 843300, Xinjiang ,China; Key Laboratory of Tarim Animal Husbandry Science and Technology, Xinjiang Production and Construction Corps, Alar 843300, Xinjiang ,ChinaCollege of life science and technology, Tarim University, Alar 843300, Xinjiang, China; College of animal science and technology, Alar 843300, Xinjiang, China; Engineering Laboratory of Tarim Animal Diseases Diagnosis and Control, Xinjiang Production and Construction Corps, Alar 843300, Xinjiang, ChinaCollege of animal science and technology, Alar 843300, Xinjiang, China; Key Laboratory of Tarim Animal Husbandry Science and Technology, Xinjiang Production and Construction Corps, Alar 843300, Xinjiang ,China; Engineering Laboratory of Tarim Animal Diseases Diagnosis and Control, Xinjiang Production and Construction Corps, Alar 843300, Xinjiang, ChinaCollege of animal science and technology, Alar 843300, Xinjiang, China; Key Laboratory of Tarim Animal Husbandry Science and Technology, Xinjiang Production and Construction Corps, Alar 843300, Xinjiang ,China; Engineering Laboratory of Tarim Animal Diseases Diagnosis and Control, Xinjiang Production and Construction Corps, Alar 843300, Xinjiang, ChinaCollege of animal science and technology, Alar 843300, Xinjiang, China; Key Laboratory of Tarim Animal Husbandry Science and Technology, Xinjiang Production and Construction Corps, Alar 843300, Xinjiang ,China; Engineering Laboratory of Tarim Animal Diseases Diagnosis and Control, Xinjiang Production and Construction Corps, Alar 843300, Xinjiang, ChinaCollege of animal science and technology, Alar 843300, Xinjiang, China; Key Laboratory of Tarim Animal Husbandry Science and Technology, Xinjiang Production and Construction Corps, Alar 843300, Xinjiang ,China; Engineering Laboratory of Tarim Animal Diseases Diagnosis and Control, Xinjiang Production and Construction Corps, Alar 843300, Xinjiang, ChinaCollege of animal science and technology, Alar 843300, Xinjiang, China; Key Laboratory of Tarim Animal Husbandry Science and Technology, Xinjiang Production and Construction Corps, Alar 843300, Xinjiang ,China; Engineering Laboratory of Tarim Animal Diseases Diagnosis and Control, Xinjiang Production and Construction Corps, Alar 843300, Xinjiang, ChinaCollege of animal science and technology, Alar 843300, Xinjiang, China; Key Laboratory of Tarim Animal Husbandry Science and Technology, Xinjiang Production and Construction Corps, Alar 843300, Xinjiang ,China; Engineering Laboratory of Tarim Animal Diseases Diagnosis and Control, Xinjiang Production and Construction Corps, Alar 843300, Xinjiang, ChinaCollege of animal science and technology, Alar 843300, Xinjiang, China; Key Laboratory of Tarim Animal Husbandry Science and Technology, Xinjiang Production and Construction Corps, Alar 843300, Xinjiang ,China; Engineering Laboratory of Tarim Animal Diseases Diagnosis and Control, Xinjiang Production and Construction Corps, Alar 843300, Xinjiang, ChinaCollege of animal science and technology, Alar 843300, Xinjiang, China; Key Laboratory of Tarim Animal Husbandry Science and Technology, Xinjiang Production and Construction Corps, Alar 843300, Xinjiang ,China; Engineering Laboratory of Tarim Animal Diseases Diagnosis and Control, Xinjiang Production and Construction Corps, Alar 843300, Xinjiang, ChinaCollege of animal science and technology, Alar 843300, Xinjiang, China; Key Laboratory of Tarim Animal Husbandry Science and Technology, Xinjiang Production and Construction Corps, Alar 843300, Xinjiang ,China; Engineering Laboratory of Tarim Animal Diseases Diagnosis and Control, Xinjiang Production and Construction Corps, Alar 843300, Xinjiang, ChinaCollege of life science and technology, Tarim University, Alar 843300, Xinjiang, China; College of animal science and technology, Alar 843300, Xinjiang, China; Key Laboratory of Tarim Animal Husbandry Science and Technology, Xinjiang Production and Construction Corps, Alar 843300, Xinjiang ,China; Engineering Laboratory of Tarim Animal Diseases Diagnosis and Control, Xinjiang Production and Construction Corps, Alar 843300, Xinjiang, China; Corresponding author:Hepatic steatosis is a highly prevalent liver disease, yet research on it is hampered by the lack of tractable cellular models in poultry. To examine the possibility of using organoids to model steatosis and detect it efficiently in leghorn male hepatocellular (LMH) cells, we first established steatosis using different concentrations of oleic acid (OA) (0.05–0.75 mmol/L) for 12 or 24 h. The subsequent detections found that the treatment of LMH cells with OA resulted in a dramatic increase in intracellular triglyceride (TG) concentrations, which was positively associated with the concentration of the inducing OA (R2 > 0.9). Then, the modeled steatosis was detected by flow cytometry after NileRed staining and it was found that the intensity of NileRed-A was positively correlated with the TG concentration (R2 > 0.93), which demonstrates that the flow cytometry is suitable for the detection of steatosis in LMH cells. According to the detection results of the different steatosis models, we confirmed that the optimal induction condition for the establishment of the steatosis model in LMH cells is OA (0.375 mmol/L) incubation for 12 h. Finally, the transcription and protein content of fat metabolism-related genes in steatosis model cells were detected. It was found that OA-induced steatosis could significantly decrease the expression of nuclear receptor PPAR-γ and the synthesis of fatty acids (SREBP-1C, ACC1, FASN), increasing the oxidative decomposition of triglycerides (CPT1A) and the assembly of low-density lipoproteins (MTTP, ApoB). Sterol metabolism in model cells was also significantly enhanced (HMGR, ABCA1, L-BABP). This study established, detected, and analyzed an OA-induced steatosis model in LMH cells, which provides a stable model and detection method for the study of poultry steatosis-related diseases.http://www.sciencedirect.com/science/article/pii/S0032579122005922LMH cellssteatosis modeloleic acidflow cytometrylipid metabolism |
| spellingShingle | Huiqi Song Ruizhi Yang Jiahao Zhang Pengliang Sun Xiaoyue Xing Lan Wang Ta Sairijima Yahui Hu Yang Liu Huixu Cheng Qiulin Zhang Lianrui Li Oleic acid-induced steatosis model establishment in LMH cells and its effect on lipid metabolism Poultry Science LMH cells steatosis model oleic acid flow cytometry lipid metabolism |
| title | Oleic acid-induced steatosis model establishment in LMH cells and its effect on lipid metabolism |
| title_full | Oleic acid-induced steatosis model establishment in LMH cells and its effect on lipid metabolism |
| title_fullStr | Oleic acid-induced steatosis model establishment in LMH cells and its effect on lipid metabolism |
| title_full_unstemmed | Oleic acid-induced steatosis model establishment in LMH cells and its effect on lipid metabolism |
| title_short | Oleic acid-induced steatosis model establishment in LMH cells and its effect on lipid metabolism |
| title_sort | oleic acid induced steatosis model establishment in lmh cells and its effect on lipid metabolism |
| topic | LMH cells steatosis model oleic acid flow cytometry lipid metabolism |
| url | http://www.sciencedirect.com/science/article/pii/S0032579122005922 |
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