Alteration of Pituitary Tumor Transforming Gene 1 by MicroRNA-186 and 655 Regulates Invasion Ability of Human Oral Squamous Cell Carcinoma
Background: Pituitary tumor-transforming gene 1 (PTTG1) was recently shown to be involved in the progression as well as the metastasis of cancers. However, their expression and function in the invasion of oral squamous cell carcinoma (SCC) remain unclear. Methods: The expressions of PTTG1 and PTTG1-...
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| Format: | Article |
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MDPI AG
2021-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/3/1021 |
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| author | Sang Shin Lee Jong Ho Choi Seung Mook Lim Gi Jin Kim Suk Keun Lee Yoon Kyung Jeon |
| author_facet | Sang Shin Lee Jong Ho Choi Seung Mook Lim Gi Jin Kim Suk Keun Lee Yoon Kyung Jeon |
| author_sort | Sang Shin Lee |
| collection | DOAJ |
| description | Background: Pituitary tumor-transforming gene 1 (PTTG1) was recently shown to be involved in the progression as well as the metastasis of cancers. However, their expression and function in the invasion of oral squamous cell carcinoma (SCC) remain unclear. Methods: The expressions of PTTG1 and PTTG1-targeted miRNA in oral SCC cell lines and their invasion capability depended on PTTG1 expression were analyzed by quantitative RT-PCR, Western blots, the transwell insert system and Zymography. Results: Invasion abilities were decreased in oral SCC cells treated with siRNA-PTTG1. When PTTG1 were downregulated in oral SCC cells treated with microRNA-186 and -655 inhibited their invasion abilities via MMP-9 activity. Conclusions: These results indicate that alteration of expression of PTTG1 in oral SCC cells by newly identified microRNA-186 and -655 can regulate invasion activity. Therefore, these data offer new insights into further understanding PTTG1 function in oral SCC and should provide new strategies for diagnostic markers for oral SCC. |
| first_indexed | 2024-03-09T04:10:33Z |
| format | Article |
| id | doaj.art-4a98cdd44c7344098a258eef31b7a0e8 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T04:10:33Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-4a98cdd44c7344098a258eef31b7a0e82023-12-03T14:00:34ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-01-01223102110.3390/ijms22031021Alteration of Pituitary Tumor Transforming Gene 1 by MicroRNA-186 and 655 Regulates Invasion Ability of Human Oral Squamous Cell CarcinomaSang Shin Lee0Jong Ho Choi1Seung Mook Lim2Gi Jin Kim3Suk Keun Lee4Yoon Kyung Jeon5Department of Oral Pathology, College of Dentistry, Gangneung-Wonju National University, Gangneung 25457, KoreaDepartment of Oral Pathology, College of Dentistry, Gangneung-Wonju National University, Gangneung 25457, KoreaDepartment of Biomedical Science, CHA University, Seoul 13488, KoreaDepartment of Biomedical Science, CHA University, Seoul 13488, KoreaDepartment of Oral Pathology, College of Dentistry, Gangneung-Wonju National University, Gangneung 25457, KoreaDepartment of Pathology, Seoul National University College of Medicine, Seoul 03080, KoreaBackground: Pituitary tumor-transforming gene 1 (PTTG1) was recently shown to be involved in the progression as well as the metastasis of cancers. However, their expression and function in the invasion of oral squamous cell carcinoma (SCC) remain unclear. Methods: The expressions of PTTG1 and PTTG1-targeted miRNA in oral SCC cell lines and their invasion capability depended on PTTG1 expression were analyzed by quantitative RT-PCR, Western blots, the transwell insert system and Zymography. Results: Invasion abilities were decreased in oral SCC cells treated with siRNA-PTTG1. When PTTG1 were downregulated in oral SCC cells treated with microRNA-186 and -655 inhibited their invasion abilities via MMP-9 activity. Conclusions: These results indicate that alteration of expression of PTTG1 in oral SCC cells by newly identified microRNA-186 and -655 can regulate invasion activity. Therefore, these data offer new insights into further understanding PTTG1 function in oral SCC and should provide new strategies for diagnostic markers for oral SCC.https://www.mdpi.com/1422-0067/22/3/1021pituitary tumor-transforming gene 1oral squamous cell carcinomainvasionmicroRNA |
| spellingShingle | Sang Shin Lee Jong Ho Choi Seung Mook Lim Gi Jin Kim Suk Keun Lee Yoon Kyung Jeon Alteration of Pituitary Tumor Transforming Gene 1 by MicroRNA-186 and 655 Regulates Invasion Ability of Human Oral Squamous Cell Carcinoma International Journal of Molecular Sciences pituitary tumor-transforming gene 1 oral squamous cell carcinoma invasion microRNA |
| title | Alteration of Pituitary Tumor Transforming Gene 1 by MicroRNA-186 and 655 Regulates Invasion Ability of Human Oral Squamous Cell Carcinoma |
| title_full | Alteration of Pituitary Tumor Transforming Gene 1 by MicroRNA-186 and 655 Regulates Invasion Ability of Human Oral Squamous Cell Carcinoma |
| title_fullStr | Alteration of Pituitary Tumor Transforming Gene 1 by MicroRNA-186 and 655 Regulates Invasion Ability of Human Oral Squamous Cell Carcinoma |
| title_full_unstemmed | Alteration of Pituitary Tumor Transforming Gene 1 by MicroRNA-186 and 655 Regulates Invasion Ability of Human Oral Squamous Cell Carcinoma |
| title_short | Alteration of Pituitary Tumor Transforming Gene 1 by MicroRNA-186 and 655 Regulates Invasion Ability of Human Oral Squamous Cell Carcinoma |
| title_sort | alteration of pituitary tumor transforming gene 1 by microrna 186 and 655 regulates invasion ability of human oral squamous cell carcinoma |
| topic | pituitary tumor-transforming gene 1 oral squamous cell carcinoma invasion microRNA |
| url | https://www.mdpi.com/1422-0067/22/3/1021 |
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