Inhibition of Quorum Sensing and Virulence Factors of <i>Pseudomonas aeruginosa</i> by Biologically Synthesized Gold and Selenium Nanoparticles
The development of microbial resistance requires a novel approach to control microbial infection. This study implies the microbial synthesis of nanometals and assessment of their antivirulent activity against <i>Pseudomonas aeruginosa</i>. <i>Streptomyces</i> isolate S91 was...
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| Format: | Article |
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MDPI AG
2021-11-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/10/12/1461 |
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| author | Soha Lotfy Elshaer Mona I. Shaaban |
| author_facet | Soha Lotfy Elshaer Mona I. Shaaban |
| author_sort | Soha Lotfy Elshaer |
| collection | DOAJ |
| description | The development of microbial resistance requires a novel approach to control microbial infection. This study implies the microbial synthesis of nanometals and assessment of their antivirulent activity against <i>Pseudomonas aeruginosa</i>. <i>Streptomyces</i> isolate S91 was isolated from soil with substantial ability for growth at high salts concentrations. The cell-free supernatant of S91was utilized for the synthesis of Au-NPs and Se-NPs. The 16S rRNA sequence analysis of <i>Streptomyces</i> S91 revealed that S91 had a high similarity (98.82%) to <i>Streptomyces olivaceous.</i> The biosynthesized Au-NPs and Se-NPs were characterized using a UV-Vis spectrophotometer, dynamic light scattering, transmission electron microscopy, energy dispersive X-ray diffraction and Fourier-transform infrared spectroscopy. The quorum sensing inhibitory (QSI) potential of Au-NPs and Se-NPs and the antivirulence activity was examined against <i>P. aeruginosa</i>. The QSI potential was confirmed using RT-PCR. The synthesized Au-NPs and Se-NPs were monodispersed spherical shapes with particle size of 12.2 and 67.98 nm, respectively. Au-NPs and Se-NPs eliminated QS in <i>P. aeruginosa</i> at a concentration range of 2.3–18.5 µg/mL for Au-NPs and 2.3–592 µg/mL for Se-NPs. In addition, Au-NPs and Se-NPs significantly inhibited QS-related virulence factors, such as pyocyanin, protease and, elastase in <i>P. aeruginosa</i>. At the molecular level, Au-NPs and Se-NPs significantly suppressed the relative expression of QS genes and toxins. Hence, the biosynthesized Au-NPS and Se-NPS could be substantial inhibitors of QS and virulence traits of <i>P. aeruginosa</i>. |
| first_indexed | 2024-03-10T04:39:26Z |
| format | Article |
| id | doaj.art-4a999dc606a5461b92a81a5d96850610 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T04:39:26Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-4a999dc606a5461b92a81a5d968506102023-11-23T03:30:11ZengMDPI AGAntibiotics2079-63822021-11-011012146110.3390/antibiotics10121461Inhibition of Quorum Sensing and Virulence Factors of <i>Pseudomonas aeruginosa</i> by Biologically Synthesized Gold and Selenium NanoparticlesSoha Lotfy Elshaer0Mona I. Shaaban1Department of Microbiology and Immunology, Faculty of Pharmacy, Mansoura University, Mansoura 35516, EgyptDepartment of Microbiology and Immunology, Faculty of Pharmacy, Mansoura University, Mansoura 35516, EgyptThe development of microbial resistance requires a novel approach to control microbial infection. This study implies the microbial synthesis of nanometals and assessment of their antivirulent activity against <i>Pseudomonas aeruginosa</i>. <i>Streptomyces</i> isolate S91 was isolated from soil with substantial ability for growth at high salts concentrations. The cell-free supernatant of S91was utilized for the synthesis of Au-NPs and Se-NPs. The 16S rRNA sequence analysis of <i>Streptomyces</i> S91 revealed that S91 had a high similarity (98.82%) to <i>Streptomyces olivaceous.</i> The biosynthesized Au-NPs and Se-NPs were characterized using a UV-Vis spectrophotometer, dynamic light scattering, transmission electron microscopy, energy dispersive X-ray diffraction and Fourier-transform infrared spectroscopy. The quorum sensing inhibitory (QSI) potential of Au-NPs and Se-NPs and the antivirulence activity was examined against <i>P. aeruginosa</i>. The QSI potential was confirmed using RT-PCR. The synthesized Au-NPs and Se-NPs were monodispersed spherical shapes with particle size of 12.2 and 67.98 nm, respectively. Au-NPs and Se-NPs eliminated QS in <i>P. aeruginosa</i> at a concentration range of 2.3–18.5 µg/mL for Au-NPs and 2.3–592 µg/mL for Se-NPs. In addition, Au-NPs and Se-NPs significantly inhibited QS-related virulence factors, such as pyocyanin, protease and, elastase in <i>P. aeruginosa</i>. At the molecular level, Au-NPs and Se-NPs significantly suppressed the relative expression of QS genes and toxins. Hence, the biosynthesized Au-NPS and Se-NPS could be substantial inhibitors of QS and virulence traits of <i>P. aeruginosa</i>.https://www.mdpi.com/2079-6382/10/12/1461Se nanoparticlesAu nanoparticlesquorum sensing inhibitionvirulence factorsantimicrobial<i>P. aeruginosa</i> |
| spellingShingle | Soha Lotfy Elshaer Mona I. Shaaban Inhibition of Quorum Sensing and Virulence Factors of <i>Pseudomonas aeruginosa</i> by Biologically Synthesized Gold and Selenium Nanoparticles Antibiotics Se nanoparticles Au nanoparticles quorum sensing inhibition virulence factors antimicrobial <i>P. aeruginosa</i> |
| title | Inhibition of Quorum Sensing and Virulence Factors of <i>Pseudomonas aeruginosa</i> by Biologically Synthesized Gold and Selenium Nanoparticles |
| title_full | Inhibition of Quorum Sensing and Virulence Factors of <i>Pseudomonas aeruginosa</i> by Biologically Synthesized Gold and Selenium Nanoparticles |
| title_fullStr | Inhibition of Quorum Sensing and Virulence Factors of <i>Pseudomonas aeruginosa</i> by Biologically Synthesized Gold and Selenium Nanoparticles |
| title_full_unstemmed | Inhibition of Quorum Sensing and Virulence Factors of <i>Pseudomonas aeruginosa</i> by Biologically Synthesized Gold and Selenium Nanoparticles |
| title_short | Inhibition of Quorum Sensing and Virulence Factors of <i>Pseudomonas aeruginosa</i> by Biologically Synthesized Gold and Selenium Nanoparticles |
| title_sort | inhibition of quorum sensing and virulence factors of i pseudomonas aeruginosa i by biologically synthesized gold and selenium nanoparticles |
| topic | Se nanoparticles Au nanoparticles quorum sensing inhibition virulence factors antimicrobial <i>P. aeruginosa</i> |
| url | https://www.mdpi.com/2079-6382/10/12/1461 |
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