A serological investigation of caseous lymphadenitis in four flocks of sheep
<p>Abstract</p> <p>A double antibody sandwich ELISA developed by ID-DLO, Lelystad to detect <it>Corynebacterium pseudotuberculosis </it>infection was used on 329 sheep from four pedigree Suffolk flocks in which clinical cases of caseous lymphadenitis (CLA) had occurred....
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2006-01-01
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| Series: | Irish Veterinary Journal |
| Subjects: | |
| Online Access: | http://www.irishvetjournal.org/content/59/1/19 |
| _version_ | 1818394122574102528 |
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| author | Malone Frank E Fee Seán A Kamp Elbarte M King David C Baird Graham J O'Reilly Kath M Murdock Fiona EA |
| author_facet | Malone Frank E Fee Seán A Kamp Elbarte M King David C Baird Graham J O'Reilly Kath M Murdock Fiona EA |
| author_sort | Malone Frank E |
| collection | DOAJ |
| description | <p>Abstract</p> <p>A double antibody sandwich ELISA developed by ID-DLO, Lelystad to detect <it>Corynebacterium pseudotuberculosis </it>infection was used on 329 sheep from four pedigree Suffolk flocks in which clinical cases of caseous lymphadenitis (CLA) had occurred. At subsequent necropsy, typical CLA lesions were seen in 133 sheep, and the diagnosis was confirmed on culture. Lesions were most commonly seen in lungs (n = 46), parotid lymph nodes (n = 44), prescapular lymph nodes (n = 38) and mediastinal lymph nodes (n = 31). The sensitivity of the ELISA test for detecting culture-positive sheep was 0.88, while the specificity of the test was 0.55. The antibody ELISA detected 87.5 <it>per cent </it>of sheep that had CLA lesions restricted to internal organs only. It was concluded that the ELISA test has a valuable role in detecting sheep with both clinical and subclinical CLA.</p> |
| first_indexed | 2024-12-14T05:56:11Z |
| format | Article |
| id | doaj.art-4a99c075249046a499f84ef693541cd8 |
| institution | Directory Open Access Journal |
| issn | 2046-0481 |
| language | English |
| last_indexed | 2024-12-14T05:56:11Z |
| publishDate | 2006-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Irish Veterinary Journal |
| spelling | doaj.art-4a99c075249046a499f84ef693541cd82022-12-21T23:14:33ZengBMCIrish Veterinary Journal2046-04812006-01-01591192110.1186/2046-0481-59-1-19A serological investigation of caseous lymphadenitis in four flocks of sheepMalone Frank EFee Seán AKamp Elbarte MKing David CBaird Graham JO'Reilly Kath MMurdock Fiona EA<p>Abstract</p> <p>A double antibody sandwich ELISA developed by ID-DLO, Lelystad to detect <it>Corynebacterium pseudotuberculosis </it>infection was used on 329 sheep from four pedigree Suffolk flocks in which clinical cases of caseous lymphadenitis (CLA) had occurred. At subsequent necropsy, typical CLA lesions were seen in 133 sheep, and the diagnosis was confirmed on culture. Lesions were most commonly seen in lungs (n = 46), parotid lymph nodes (n = 44), prescapular lymph nodes (n = 38) and mediastinal lymph nodes (n = 31). The sensitivity of the ELISA test for detecting culture-positive sheep was 0.88, while the specificity of the test was 0.55. The antibody ELISA detected 87.5 <it>per cent </it>of sheep that had CLA lesions restricted to internal organs only. It was concluded that the ELISA test has a valuable role in detecting sheep with both clinical and subclinical CLA.</p>http://www.irishvetjournal.org/content/59/1/19SheepCaseous lymphadenitisSerology<it>Corynebacterium pseudotuberculosis</it> |
| spellingShingle | Malone Frank E Fee Seán A Kamp Elbarte M King David C Baird Graham J O'Reilly Kath M Murdock Fiona EA A serological investigation of caseous lymphadenitis in four flocks of sheep Irish Veterinary Journal Sheep Caseous lymphadenitis Serology <it>Corynebacterium pseudotuberculosis</it> |
| title | A serological investigation of caseous lymphadenitis in four flocks of sheep |
| title_full | A serological investigation of caseous lymphadenitis in four flocks of sheep |
| title_fullStr | A serological investigation of caseous lymphadenitis in four flocks of sheep |
| title_full_unstemmed | A serological investigation of caseous lymphadenitis in four flocks of sheep |
| title_short | A serological investigation of caseous lymphadenitis in four flocks of sheep |
| title_sort | serological investigation of caseous lymphadenitis in four flocks of sheep |
| topic | Sheep Caseous lymphadenitis Serology <it>Corynebacterium pseudotuberculosis</it> |
| url | http://www.irishvetjournal.org/content/59/1/19 |
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