Polyphenol Composition, Antioxidant Activity and Cytotoxicity of Seeds from Two Underexploited Wild Licania Species: L. rigida and L. tomentosa
Studies have shown the benefit of antioxidants in the prevention or treatment of human diseases and promoted a growing interest in new sources of plant antioxidants for pharmacological use. This study aimed to add value to two underexploited wild plant species (Licania rigida) and L. tomentosa) from...
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MDPI AG
2016-12-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/21/12/1755 |
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| author | Igor Parra Pessoa José Joaquim Lopes Neto Thiago Silva de Almeida Davi Felipe Farias Leonardo Rogério Vieira Jackeline Lima de Medeiros Aline Augusti Boligon Ad Peijnenburg Ivan Castelar Ana Fontenele Urano Carvalho |
| author_facet | Igor Parra Pessoa José Joaquim Lopes Neto Thiago Silva de Almeida Davi Felipe Farias Leonardo Rogério Vieira Jackeline Lima de Medeiros Aline Augusti Boligon Ad Peijnenburg Ivan Castelar Ana Fontenele Urano Carvalho |
| author_sort | Igor Parra Pessoa |
| collection | DOAJ |
| description | Studies have shown the benefit of antioxidants in the prevention or treatment of human diseases and promoted a growing interest in new sources of plant antioxidants for pharmacological use. This study aimed to add value to two underexploited wild plant species (Licania rigida) and L. tomentosa) from Brazilian flora. Thus, the phenolic compounds profile of their seed ethanol extract and derived fractions were elucidated by HPLC, the antioxidant capacity was assessed by in vitro chemical tests and the cytotoxicity determined using the human carcinoma cell lines MCF-7 and Caco-2. Eleven phenolic compounds were identified in the extracts of each species. The extracts and fractions showed excellent antioxidant activity in the DPPH assay (SC50, ranging from 9.15 to 248.8 µg/mL). The aqueous fraction of L. rigida seeds was most effective in preventing lipid peroxidation under basal conditions (IC50 60.80 µg/mL) whereas, in the presence of stress inducer, the methanolic fraction of L. tomentosa performed best (IC50 8.55 µg/mL). None of the samples showed iron chelating capacity. Ethanolic seed extracts of both species did not reveal any cytotoxicity against MCF-7 and Caco-2 cells. Both plant species showed a promising phenolic profile with potent antioxidant capacity and deserve attention to be sustainably explored. |
| first_indexed | 2024-04-12T21:29:59Z |
| format | Article |
| id | doaj.art-4a9ad7d4543043d1a1e9583d688fc407 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-12T21:29:59Z |
| publishDate | 2016-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-4a9ad7d4543043d1a1e9583d688fc4072022-12-22T03:16:03ZengMDPI AGMolecules1420-30492016-12-012112175510.3390/molecules21121755molecules21121755Polyphenol Composition, Antioxidant Activity and Cytotoxicity of Seeds from Two Underexploited Wild Licania Species: L. rigida and L. tomentosaIgor Parra Pessoa0José Joaquim Lopes Neto1Thiago Silva de Almeida2Davi Felipe Farias3Leonardo Rogério Vieira4Jackeline Lima de Medeiros5Aline Augusti Boligon6Ad Peijnenburg7Ivan Castelar8Ana Fontenele Urano Carvalho9Department of Biochemistry and Molecular Biology, Federal University of Ceará, 60020-181 Fortaleza, CE, BrazilDepartment of Biochemistry and Molecular Biology, Federal University of Ceará, 60020-181 Fortaleza, CE, BrazilDepartment of Biochemistry and Molecular Biology, Federal University of Ceará, 60020-181 Fortaleza, CE, BrazilDepartment of Molecular Biology, Federal University of Paraíba, 58051-900 João Pessoa, PB, BrazilDepartment of Biochemistry and Molecular Biology, Federal University of Ceará, 60020-181 Fortaleza, CE, BrazilDepartment of Biochemistry and Molecular Biology, Federal University of Ceará, 60020-181 Fortaleza, CE, BrazilHealth Sciences Center, Federal University of Santa Maria, 97105-900 Santa Maria, RS, BrazilRIKILT, Wageningen University and Research, P.O. Box 230, 6700 AE Wageningen, The NetherlandsDepartment of Finance, Federal University of Ceará, 60020-181 Fortaleza, CE, BrazilDepartment of Biochemistry and Molecular Biology, Federal University of Ceará, 60020-181 Fortaleza, CE, BrazilStudies have shown the benefit of antioxidants in the prevention or treatment of human diseases and promoted a growing interest in new sources of plant antioxidants for pharmacological use. This study aimed to add value to two underexploited wild plant species (Licania rigida) and L. tomentosa) from Brazilian flora. Thus, the phenolic compounds profile of their seed ethanol extract and derived fractions were elucidated by HPLC, the antioxidant capacity was assessed by in vitro chemical tests and the cytotoxicity determined using the human carcinoma cell lines MCF-7 and Caco-2. Eleven phenolic compounds were identified in the extracts of each species. The extracts and fractions showed excellent antioxidant activity in the DPPH assay (SC50, ranging from 9.15 to 248.8 µg/mL). The aqueous fraction of L. rigida seeds was most effective in preventing lipid peroxidation under basal conditions (IC50 60.80 µg/mL) whereas, in the presence of stress inducer, the methanolic fraction of L. tomentosa performed best (IC50 8.55 µg/mL). None of the samples showed iron chelating capacity. Ethanolic seed extracts of both species did not reveal any cytotoxicity against MCF-7 and Caco-2 cells. Both plant species showed a promising phenolic profile with potent antioxidant capacity and deserve attention to be sustainably explored.http://www.mdpi.com/1420-3049/21/12/1755MCF-7Caco-2DPPHTBARSphenolic compoundcytotoxicity |
| spellingShingle | Igor Parra Pessoa José Joaquim Lopes Neto Thiago Silva de Almeida Davi Felipe Farias Leonardo Rogério Vieira Jackeline Lima de Medeiros Aline Augusti Boligon Ad Peijnenburg Ivan Castelar Ana Fontenele Urano Carvalho Polyphenol Composition, Antioxidant Activity and Cytotoxicity of Seeds from Two Underexploited Wild Licania Species: L. rigida and L. tomentosa Molecules MCF-7 Caco-2 DPPH TBARS phenolic compound cytotoxicity |
| title | Polyphenol Composition, Antioxidant Activity and Cytotoxicity of Seeds from Two Underexploited Wild Licania Species: L. rigida and L. tomentosa |
| title_full | Polyphenol Composition, Antioxidant Activity and Cytotoxicity of Seeds from Two Underexploited Wild Licania Species: L. rigida and L. tomentosa |
| title_fullStr | Polyphenol Composition, Antioxidant Activity and Cytotoxicity of Seeds from Two Underexploited Wild Licania Species: L. rigida and L. tomentosa |
| title_full_unstemmed | Polyphenol Composition, Antioxidant Activity and Cytotoxicity of Seeds from Two Underexploited Wild Licania Species: L. rigida and L. tomentosa |
| title_short | Polyphenol Composition, Antioxidant Activity and Cytotoxicity of Seeds from Two Underexploited Wild Licania Species: L. rigida and L. tomentosa |
| title_sort | polyphenol composition antioxidant activity and cytotoxicity of seeds from two underexploited wild licania species l rigida and l tomentosa |
| topic | MCF-7 Caco-2 DPPH TBARS phenolic compound cytotoxicity |
| url | http://www.mdpi.com/1420-3049/21/12/1755 |
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