<i>Escherichia coli</i> O157:H7 Curli Fimbriae Promotes Biofilm Formation, Epithelial Cell Invasion, and Persistence in Cattle
<i>Escherichia coli</i> O157:H7 (O157) is noninvasive and a weak biofilm producer; however, a subset of O157 are exceptions. O157 ATCC 43895 forms biofilms and invades epithelial cells. Tn<i>5</i> mutagenesis identified a mutation responsible for both phenotypes. The insertio...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-04-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/4/580 |
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| author | Haiqing Sheng Yansong Xue Wei Zhao Carolyn J. Hovde Scott A. Minnich |
| author_facet | Haiqing Sheng Yansong Xue Wei Zhao Carolyn J. Hovde Scott A. Minnich |
| author_sort | Haiqing Sheng |
| collection | DOAJ |
| description | <i>Escherichia coli</i> O157:H7 (O157) is noninvasive and a weak biofilm producer; however, a subset of O157 are exceptions. O157 ATCC 43895 forms biofilms and invades epithelial cells. Tn<i>5</i> mutagenesis identified a mutation responsible for both phenotypes. The insertion mapped within the curli <i>csgB</i> fimbriae locus. Screening of O157 strains for biofilm formation and cell invasion identified a bovine and a clinical isolate with those characteristics. A single base pair A to T transversion, intergenic to the curli divergent operons <i>csgDEFG</i> and <i>csgBAC</i>, was present only in biofilm-producing and invasive strains. Using site-directed mutagenesis, this single base change was introduced into two curli-negative/noninvasive O157 strains and modified strains to form biofilms, produce curli, and gain invasive capability. Transmission electron microscopy (EM) and immuno-EM confirmed curli fibers. EM of bovine epithelial cells (MAC-T) co-cultured with curli-expressing O157 showed intracellular bacteria. The role of curli in O157 persistence in cattle was examined by challenging cattle with curli-positive and -negative O157 and comparing carriage. The duration of bovine colonization with the O157 curli-negative mutant was shorter than its curli-positive isogenic parent. These findings definitively demonstrate that a single base pair stably confers biofilm formation, epithelial cell invasion, and persistence in cattle. |
| first_indexed | 2024-03-10T20:25:01Z |
| format | Article |
| id | doaj.art-4a9b37273ce240dfa9c088de2069a9cf |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T20:25:01Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-4a9b37273ce240dfa9c088de2069a9cf2023-11-19T21:53:16ZengMDPI AGMicroorganisms2076-26072020-04-018458010.3390/microorganisms8040580<i>Escherichia coli</i> O157:H7 Curli Fimbriae Promotes Biofilm Formation, Epithelial Cell Invasion, and Persistence in CattleHaiqing Sheng0Yansong Xue1Wei Zhao2Carolyn J. Hovde3Scott A. Minnich4Bi-State School of Food Science, University of Idaho, Moscow, ID 83844-2312, USABi-State School of Food Science, University of Idaho, Moscow, ID 83844-2312, USABi-State School of Food Science, University of Idaho, Moscow, ID 83844-2312, USABi-State School of Food Science, University of Idaho, Moscow, ID 83844-2312, USABi-State School of Food Science, University of Idaho, Moscow, ID 83844-2312, USA<i>Escherichia coli</i> O157:H7 (O157) is noninvasive and a weak biofilm producer; however, a subset of O157 are exceptions. O157 ATCC 43895 forms biofilms and invades epithelial cells. Tn<i>5</i> mutagenesis identified a mutation responsible for both phenotypes. The insertion mapped within the curli <i>csgB</i> fimbriae locus. Screening of O157 strains for biofilm formation and cell invasion identified a bovine and a clinical isolate with those characteristics. A single base pair A to T transversion, intergenic to the curli divergent operons <i>csgDEFG</i> and <i>csgBAC</i>, was present only in biofilm-producing and invasive strains. Using site-directed mutagenesis, this single base change was introduced into two curli-negative/noninvasive O157 strains and modified strains to form biofilms, produce curli, and gain invasive capability. Transmission electron microscopy (EM) and immuno-EM confirmed curli fibers. EM of bovine epithelial cells (MAC-T) co-cultured with curli-expressing O157 showed intracellular bacteria. The role of curli in O157 persistence in cattle was examined by challenging cattle with curli-positive and -negative O157 and comparing carriage. The duration of bovine colonization with the O157 curli-negative mutant was shorter than its curli-positive isogenic parent. These findings definitively demonstrate that a single base pair stably confers biofilm formation, epithelial cell invasion, and persistence in cattle.https://www.mdpi.com/2076-2607/8/4/580curli<i>E. coli</i> O157biofilminvasioncattle |
| spellingShingle | Haiqing Sheng Yansong Xue Wei Zhao Carolyn J. Hovde Scott A. Minnich <i>Escherichia coli</i> O157:H7 Curli Fimbriae Promotes Biofilm Formation, Epithelial Cell Invasion, and Persistence in Cattle Microorganisms curli <i>E. coli</i> O157 biofilm invasion cattle |
| title | <i>Escherichia coli</i> O157:H7 Curli Fimbriae Promotes Biofilm Formation, Epithelial Cell Invasion, and Persistence in Cattle |
| title_full | <i>Escherichia coli</i> O157:H7 Curli Fimbriae Promotes Biofilm Formation, Epithelial Cell Invasion, and Persistence in Cattle |
| title_fullStr | <i>Escherichia coli</i> O157:H7 Curli Fimbriae Promotes Biofilm Formation, Epithelial Cell Invasion, and Persistence in Cattle |
| title_full_unstemmed | <i>Escherichia coli</i> O157:H7 Curli Fimbriae Promotes Biofilm Formation, Epithelial Cell Invasion, and Persistence in Cattle |
| title_short | <i>Escherichia coli</i> O157:H7 Curli Fimbriae Promotes Biofilm Formation, Epithelial Cell Invasion, and Persistence in Cattle |
| title_sort | i escherichia coli i o157 h7 curli fimbriae promotes biofilm formation epithelial cell invasion and persistence in cattle |
| topic | curli <i>E. coli</i> O157 biofilm invasion cattle |
| url | https://www.mdpi.com/2076-2607/8/4/580 |
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