Platelet Activation Favours NOX2-Mediated Muscle Damage in Elite Athletes: The Role of Cocoa-Derived Polyphenols
Mechanisms of exercise-induced muscle injury with etiopathogenesis and its consequences have been described; however, the impact of different intensities of exercise on the mechanisms of muscular injury development is not well understood. The aim of this study was to exploit the relationship between...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-04-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/14/8/1558 |
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| author | Alessandra D’Amico Elena Cavarretta Chiara Fossati Paolo Borrione Fabio Pigozzi Giacomo Frati Sebastiano Sciarretta Vincenzo Costa Fabrizio De Grandis Antonia Nigro Mariangela Peruzzi Fabio Miraldi Wael Saade Antonella Calogero Paolo Rosa Gioacchino Galardo Lorenzo Loffredo Pasquale Pignatelli Cristina Nocella Roberto Carnevale |
| author_facet | Alessandra D’Amico Elena Cavarretta Chiara Fossati Paolo Borrione Fabio Pigozzi Giacomo Frati Sebastiano Sciarretta Vincenzo Costa Fabrizio De Grandis Antonia Nigro Mariangela Peruzzi Fabio Miraldi Wael Saade Antonella Calogero Paolo Rosa Gioacchino Galardo Lorenzo Loffredo Pasquale Pignatelli Cristina Nocella Roberto Carnevale |
| author_sort | Alessandra D’Amico |
| collection | DOAJ |
| description | Mechanisms of exercise-induced muscle injury with etiopathogenesis and its consequences have been described; however, the impact of different intensities of exercise on the mechanisms of muscular injury development is not well understood. The aim of this study was to exploit the relationship between platelet activation, oxidative stress and muscular injuries induced by physical exercise in elite football players compared to amateur athletes. Oxidant/antioxidant status, platelet activation and markers of muscle damage were evaluated in 23 elite football players and 23 amateur athletes. Compared to amateurs, elite football players showed lower antioxidant capacity and higher oxidative stress paralleled by increased platelet activation and muscle damage markers. Simple linear regression analysis showed that sNOX2-dp and H<sub>2</sub>O<sub>2</sub>, sCD40L and PDGF-bb were associated with a significant increase in muscle damage biomarkers. In vitro studies also showed that plasma obtained from elite athletes increased oxidative stress and muscle damage in human skeletal muscle myoblasts cell line compared to amateurs’ plasma, an effect blunted by the NOX2 inhibitor or by the cell treatment with cocoa-derived polyphenols. These results indicate that platelet activation increased muscular injuries induced by oxidative stress. Moreover, NOX2 inhibition and polyphenol extracts treatment positively modulates redox status and reduce exercise-induced muscular injury. |
| first_indexed | 2024-03-09T04:20:48Z |
| format | Article |
| id | doaj.art-4a9c04c28ce848d99d57607a7b71f947 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-09T04:20:48Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-4a9c04c28ce848d99d57607a7b71f9472023-12-03T13:48:03ZengMDPI AGNutrients2072-66432022-04-01148155810.3390/nu14081558Platelet Activation Favours NOX2-Mediated Muscle Damage in Elite Athletes: The Role of Cocoa-Derived PolyphenolsAlessandra D’Amico0Elena Cavarretta1Chiara Fossati2Paolo Borrione3Fabio Pigozzi4Giacomo Frati5Sebastiano Sciarretta6Vincenzo Costa7Fabrizio De Grandis8Antonia Nigro9Mariangela Peruzzi10Fabio Miraldi11Wael Saade12Antonella Calogero13Paolo Rosa14Gioacchino Galardo15Lorenzo Loffredo16Pasquale Pignatelli17Cristina Nocella18Roberto Carnevale19Department of Movement, Human and Health Sciences, University of Rome “Foro Italico”, 00135 Rome, ItalyDepartment of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome, 04100 Latina, ItalyDepartment of Movement, Human and Health Sciences, University of Rome “Foro Italico”, 00135 Rome, ItalyDepartment of Movement, Human and Health Sciences, University of Rome “Foro Italico”, 00135 Rome, ItalyDepartment of Movement, Human and Health Sciences, University of Rome “Foro Italico”, 00135 Rome, ItalyDepartment of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome, 04100 Latina, ItalyDepartment of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome, 04100 Latina, ItalyAS Roma Football Club, Piazzale Dino Viola 1, 00128 Rome, ItalyVilla Stuart Sport Clinic, FIFA Medical Center of Excellence, 00135 Rome, ItalyVilla Stuart Sport Clinic, FIFA Medical Center of Excellence, 00135 Rome, ItalyMediterranea, Cardiocentro, 80122 Napoli, ItalyDepartment of Clinical, Internal, Anesthesiological and Cardiovascular Sciences, Sapienza University of Rome, 00161 Rome, ItalyDepartment of Clinical, Internal, Anesthesiological and Cardiovascular Sciences, Sapienza University of Rome, 00161 Rome, ItalyDepartment of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome, 04100 Latina, ItalyDepartment of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome, 04100 Latina, ItalyPoliclinico Umberto I, Viale Regina Elena, 328, 00161 Rome, ItalyDepartment of Clinical, Internal, Anesthesiological and Cardiovascular Sciences, Sapienza University of Rome, 00161 Rome, ItalyMediterranea, Cardiocentro, 80122 Napoli, ItalyDepartment of Clinical, Internal, Anesthesiological and Cardiovascular Sciences, Sapienza University of Rome, 00161 Rome, ItalyDepartment of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome, 04100 Latina, ItalyMechanisms of exercise-induced muscle injury with etiopathogenesis and its consequences have been described; however, the impact of different intensities of exercise on the mechanisms of muscular injury development is not well understood. The aim of this study was to exploit the relationship between platelet activation, oxidative stress and muscular injuries induced by physical exercise in elite football players compared to amateur athletes. Oxidant/antioxidant status, platelet activation and markers of muscle damage were evaluated in 23 elite football players and 23 amateur athletes. Compared to amateurs, elite football players showed lower antioxidant capacity and higher oxidative stress paralleled by increased platelet activation and muscle damage markers. Simple linear regression analysis showed that sNOX2-dp and H<sub>2</sub>O<sub>2</sub>, sCD40L and PDGF-bb were associated with a significant increase in muscle damage biomarkers. In vitro studies also showed that plasma obtained from elite athletes increased oxidative stress and muscle damage in human skeletal muscle myoblasts cell line compared to amateurs’ plasma, an effect blunted by the NOX2 inhibitor or by the cell treatment with cocoa-derived polyphenols. These results indicate that platelet activation increased muscular injuries induced by oxidative stress. Moreover, NOX2 inhibition and polyphenol extracts treatment positively modulates redox status and reduce exercise-induced muscular injury.https://www.mdpi.com/2072-6643/14/8/1558oxidative stressexerciseplatelet activationpolyphenols |
| spellingShingle | Alessandra D’Amico Elena Cavarretta Chiara Fossati Paolo Borrione Fabio Pigozzi Giacomo Frati Sebastiano Sciarretta Vincenzo Costa Fabrizio De Grandis Antonia Nigro Mariangela Peruzzi Fabio Miraldi Wael Saade Antonella Calogero Paolo Rosa Gioacchino Galardo Lorenzo Loffredo Pasquale Pignatelli Cristina Nocella Roberto Carnevale Platelet Activation Favours NOX2-Mediated Muscle Damage in Elite Athletes: The Role of Cocoa-Derived Polyphenols Nutrients oxidative stress exercise platelet activation polyphenols |
| title | Platelet Activation Favours NOX2-Mediated Muscle Damage in Elite Athletes: The Role of Cocoa-Derived Polyphenols |
| title_full | Platelet Activation Favours NOX2-Mediated Muscle Damage in Elite Athletes: The Role of Cocoa-Derived Polyphenols |
| title_fullStr | Platelet Activation Favours NOX2-Mediated Muscle Damage in Elite Athletes: The Role of Cocoa-Derived Polyphenols |
| title_full_unstemmed | Platelet Activation Favours NOX2-Mediated Muscle Damage in Elite Athletes: The Role of Cocoa-Derived Polyphenols |
| title_short | Platelet Activation Favours NOX2-Mediated Muscle Damage in Elite Athletes: The Role of Cocoa-Derived Polyphenols |
| title_sort | platelet activation favours nox2 mediated muscle damage in elite athletes the role of cocoa derived polyphenols |
| topic | oxidative stress exercise platelet activation polyphenols |
| url | https://www.mdpi.com/2072-6643/14/8/1558 |
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