Interaction of detergents with biological membranes: Comparison of fluorescence assays with filtration protocols and implications for the rates of detergent association, dissociation and flip-flop.
The present study mainly consists of a re-evaluation of the rate at which C12E8, a typical non-ionic detergent used for membrane studies, is able to dissociate from biological membranes, with sarcoplasmic reticulum membrane vesicles being used as an example. Utilizing a brominated derivative of C12E...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2019-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0222932 |
| _version_ | 1828957342652170240 |
|---|---|
| author | Philippe Champeil Béatrice de Foresta Martin Picard Carole Gauron Dominique Georgin Marc le Maire Jesper V Møller Guillaume Lenoir Cédric Montigny |
| author_facet | Philippe Champeil Béatrice de Foresta Martin Picard Carole Gauron Dominique Georgin Marc le Maire Jesper V Møller Guillaume Lenoir Cédric Montigny |
| author_sort | Philippe Champeil |
| collection | DOAJ |
| description | The present study mainly consists of a re-evaluation of the rate at which C12E8, a typical non-ionic detergent used for membrane studies, is able to dissociate from biological membranes, with sarcoplasmic reticulum membrane vesicles being used as an example. Utilizing a brominated derivative of C12E8 and now stopped-flow fluorescence instead of rapid filtration, we found that the rate of dissociation of this detergent from these membranes, merely perturbed with non-solubilizing concentrations of detergent, was significantly faster (t1/2 < 10 ms) than what had previously been determined (t1/2 ~300-400 ms) from experiments based on a rapid filtration protocol using 14C-labeled C12E8 and glass fiber filters (Binding of a non-ionic detergent to membranes: flip-flop rate and location on the bilayer, by Marc le Maire, Jesper Møller and Philippe Champeil, Biochemistry (1987) Vol 26, pages 4803-4810). We here pinpoint a methodological problem of the earlier rapid filtration experiments, and we suggest that the true overall dissociation rate of C12E8 is indeed much faster than previously thought. We also exemplify the case of brominated dodecyl-maltoside, whose kinetics for overall binding to and dissociation from membranes comprise both a rapid and a sower phase, the latter being presumably due to flip-flop between the two leaflets of the membrane. Consequently, equilibrium is reached only after a few seconds for DDM. This work thereby emphasizes the interest of using the fluorescence quenching associated with brominated detergents for studying the kinetics of detergent/membrane interactions, namely association, dissociation and flip-flop rates. |
| first_indexed | 2024-12-14T08:26:49Z |
| format | Article |
| id | doaj.art-4aa83949c10942028c6720c14d91f2f1 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T08:26:49Z |
| publishDate | 2019-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-4aa83949c10942028c6720c14d91f2f12022-12-21T23:09:37ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-011410e022293210.1371/journal.pone.0222932Interaction of detergents with biological membranes: Comparison of fluorescence assays with filtration protocols and implications for the rates of detergent association, dissociation and flip-flop.Philippe ChampeilBéatrice de ForestaMartin PicardCarole GauronDominique GeorginMarc le MaireJesper V MøllerGuillaume LenoirCédric MontignyThe present study mainly consists of a re-evaluation of the rate at which C12E8, a typical non-ionic detergent used for membrane studies, is able to dissociate from biological membranes, with sarcoplasmic reticulum membrane vesicles being used as an example. Utilizing a brominated derivative of C12E8 and now stopped-flow fluorescence instead of rapid filtration, we found that the rate of dissociation of this detergent from these membranes, merely perturbed with non-solubilizing concentrations of detergent, was significantly faster (t1/2 < 10 ms) than what had previously been determined (t1/2 ~300-400 ms) from experiments based on a rapid filtration protocol using 14C-labeled C12E8 and glass fiber filters (Binding of a non-ionic detergent to membranes: flip-flop rate and location on the bilayer, by Marc le Maire, Jesper Møller and Philippe Champeil, Biochemistry (1987) Vol 26, pages 4803-4810). We here pinpoint a methodological problem of the earlier rapid filtration experiments, and we suggest that the true overall dissociation rate of C12E8 is indeed much faster than previously thought. We also exemplify the case of brominated dodecyl-maltoside, whose kinetics for overall binding to and dissociation from membranes comprise both a rapid and a sower phase, the latter being presumably due to flip-flop between the two leaflets of the membrane. Consequently, equilibrium is reached only after a few seconds for DDM. This work thereby emphasizes the interest of using the fluorescence quenching associated with brominated detergents for studying the kinetics of detergent/membrane interactions, namely association, dissociation and flip-flop rates.https://doi.org/10.1371/journal.pone.0222932 |
| spellingShingle | Philippe Champeil Béatrice de Foresta Martin Picard Carole Gauron Dominique Georgin Marc le Maire Jesper V Møller Guillaume Lenoir Cédric Montigny Interaction of detergents with biological membranes: Comparison of fluorescence assays with filtration protocols and implications for the rates of detergent association, dissociation and flip-flop. PLoS ONE |
| title | Interaction of detergents with biological membranes: Comparison of fluorescence assays with filtration protocols and implications for the rates of detergent association, dissociation and flip-flop. |
| title_full | Interaction of detergents with biological membranes: Comparison of fluorescence assays with filtration protocols and implications for the rates of detergent association, dissociation and flip-flop. |
| title_fullStr | Interaction of detergents with biological membranes: Comparison of fluorescence assays with filtration protocols and implications for the rates of detergent association, dissociation and flip-flop. |
| title_full_unstemmed | Interaction of detergents with biological membranes: Comparison of fluorescence assays with filtration protocols and implications for the rates of detergent association, dissociation and flip-flop. |
| title_short | Interaction of detergents with biological membranes: Comparison of fluorescence assays with filtration protocols and implications for the rates of detergent association, dissociation and flip-flop. |
| title_sort | interaction of detergents with biological membranes comparison of fluorescence assays with filtration protocols and implications for the rates of detergent association dissociation and flip flop |
| url | https://doi.org/10.1371/journal.pone.0222932 |
| work_keys_str_mv | AT philippechampeil interactionofdetergentswithbiologicalmembranescomparisonoffluorescenceassayswithfiltrationprotocolsandimplicationsfortheratesofdetergentassociationdissociationandflipflop AT beatricedeforesta interactionofdetergentswithbiologicalmembranescomparisonoffluorescenceassayswithfiltrationprotocolsandimplicationsfortheratesofdetergentassociationdissociationandflipflop AT martinpicard interactionofdetergentswithbiologicalmembranescomparisonoffluorescenceassayswithfiltrationprotocolsandimplicationsfortheratesofdetergentassociationdissociationandflipflop AT carolegauron interactionofdetergentswithbiologicalmembranescomparisonoffluorescenceassayswithfiltrationprotocolsandimplicationsfortheratesofdetergentassociationdissociationandflipflop AT dominiquegeorgin interactionofdetergentswithbiologicalmembranescomparisonoffluorescenceassayswithfiltrationprotocolsandimplicationsfortheratesofdetergentassociationdissociationandflipflop AT marclemaire interactionofdetergentswithbiologicalmembranescomparisonoffluorescenceassayswithfiltrationprotocolsandimplicationsfortheratesofdetergentassociationdissociationandflipflop AT jespervmøller interactionofdetergentswithbiologicalmembranescomparisonoffluorescenceassayswithfiltrationprotocolsandimplicationsfortheratesofdetergentassociationdissociationandflipflop AT guillaumelenoir interactionofdetergentswithbiologicalmembranescomparisonoffluorescenceassayswithfiltrationprotocolsandimplicationsfortheratesofdetergentassociationdissociationandflipflop AT cedricmontigny interactionofdetergentswithbiologicalmembranescomparisonoffluorescenceassayswithfiltrationprotocolsandimplicationsfortheratesofdetergentassociationdissociationandflipflop |