Protective Effect of Electroacupuncture on Chemotherapy-Induced Salivary Gland Hypofunction in a Mouse Model
Radiotherapy and chemotherapy can impair salivary gland (SG) function, which causes xerostomia and exacerbate other side effects of chemotherapy and oral infection, reducing patients’ quality of life. This animal study aimed to assess the efficacy of electroacupuncture (EA) as a means of preventing...
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MDPI AG
2023-07-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/14/11654 |
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| author | Thanh-Hien Vu Nguyen Kuo-Chou Chiu Yin-Hwa Shih Chung-Ji Liu Tran Van Bao Quach Shih-Min Hsia Yi-Hung Chen Tzong-Ming Shieh |
| author_facet | Thanh-Hien Vu Nguyen Kuo-Chou Chiu Yin-Hwa Shih Chung-Ji Liu Tran Van Bao Quach Shih-Min Hsia Yi-Hung Chen Tzong-Ming Shieh |
| author_sort | Thanh-Hien Vu Nguyen |
| collection | DOAJ |
| description | Radiotherapy and chemotherapy can impair salivary gland (SG) function, which causes xerostomia and exacerbate other side effects of chemotherapy and oral infection, reducing patients’ quality of life. This animal study aimed to assess the efficacy of electroacupuncture (EA) as a means of preventing xerostomia induced by 5−fluorouracil (5−FU). A xerostomia mouse model was induced via four tail vein injections of 5−FU (80 mg/kg/dose). EA was performed at LI4 and LI11 for 7 days. The pilocarpine-stimulated salivary flow rate (SFR) and salivary glands weight (SGW) were recorded. Salivary immunoglobulin A (SIgA) and lysozyme were determined via enzyme-linked immunosorbent assay (ELISA). SG was collected for hematoxylin and eosin staining to measure acini number and acinar cell size. <i>Tumor necrosis factor</i>-<i>α (TNF</i>-<i>α)</i>, <i>interleukin</i>-<i>1β (IL</i>-<i>1β)</i>, and <i>aquaporin 5 (AQP5)</i> mRNA expressions in SG were quantified via RT-qPCR. 5−FU caused significant decreases in SFR, SGW, SIgA, lysozyme, <i>AQP5</i> expression, and acini number, while <i>TNF-α</i> and <i>IL-1β</i> expressions and acinar cell size were significantly increased. EA treatment can prevent 5−FU damage to the salivary gland, while pilocarpine treatment can only elevate SFR and AQP5 expression. These findings provide significant evidence to support the use of EA as an alternative treatment for chemotherapy-induced salivary gland hypofunction and xerostomia. |
| first_indexed | 2024-03-11T00:59:34Z |
| format | Article |
| id | doaj.art-4aab1ea3a62a4622a893494ff0342704 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T00:59:34Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-4aab1ea3a62a4622a893494ff03427042023-11-18T19:42:50ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-07-0124141165410.3390/ijms241411654Protective Effect of Electroacupuncture on Chemotherapy-Induced Salivary Gland Hypofunction in a Mouse ModelThanh-Hien Vu Nguyen0Kuo-Chou Chiu1Yin-Hwa Shih2Chung-Ji Liu3Tran Van Bao Quach4Shih-Min Hsia5Yi-Hung Chen6Tzong-Ming Shieh7Graduate Institute of Acupuncture Science, China Medical University, Taichung 40402, TaiwanDivision of Oral Diagnosis and Family Dentistry, Tri-Service General Hospital, National Defense Medical Center, Taipei 11490, TaiwanDepartment of Healthcare Administration, Asia University, Taichung 41354, TaiwanDepartment of Oral and Maxillofacial Surgery, MacKay Memorial Hospital, Taipei 10449, TaiwanSchool of Dentistry, China Medical University, Taichung 40402, TaiwanSchool of Nutrition and Health Sciences, Taipei Medical University, Taipei 110301, TaiwanGraduate Institute of Acupuncture Science, China Medical University, Taichung 40402, TaiwanSchool of Dentistry, China Medical University, Taichung 40402, TaiwanRadiotherapy and chemotherapy can impair salivary gland (SG) function, which causes xerostomia and exacerbate other side effects of chemotherapy and oral infection, reducing patients’ quality of life. This animal study aimed to assess the efficacy of electroacupuncture (EA) as a means of preventing xerostomia induced by 5−fluorouracil (5−FU). A xerostomia mouse model was induced via four tail vein injections of 5−FU (80 mg/kg/dose). EA was performed at LI4 and LI11 for 7 days. The pilocarpine-stimulated salivary flow rate (SFR) and salivary glands weight (SGW) were recorded. Salivary immunoglobulin A (SIgA) and lysozyme were determined via enzyme-linked immunosorbent assay (ELISA). SG was collected for hematoxylin and eosin staining to measure acini number and acinar cell size. <i>Tumor necrosis factor</i>-<i>α (TNF</i>-<i>α)</i>, <i>interleukin</i>-<i>1β (IL</i>-<i>1β)</i>, and <i>aquaporin 5 (AQP5)</i> mRNA expressions in SG were quantified via RT-qPCR. 5−FU caused significant decreases in SFR, SGW, SIgA, lysozyme, <i>AQP5</i> expression, and acini number, while <i>TNF-α</i> and <i>IL-1β</i> expressions and acinar cell size were significantly increased. EA treatment can prevent 5−FU damage to the salivary gland, while pilocarpine treatment can only elevate SFR and AQP5 expression. These findings provide significant evidence to support the use of EA as an alternative treatment for chemotherapy-induced salivary gland hypofunction and xerostomia.https://www.mdpi.com/1422-0067/24/14/11654xerostomiasalivary hypofunctionchemotherapy5-fluorouracilinflammationacupuncture |
| spellingShingle | Thanh-Hien Vu Nguyen Kuo-Chou Chiu Yin-Hwa Shih Chung-Ji Liu Tran Van Bao Quach Shih-Min Hsia Yi-Hung Chen Tzong-Ming Shieh Protective Effect of Electroacupuncture on Chemotherapy-Induced Salivary Gland Hypofunction in a Mouse Model International Journal of Molecular Sciences xerostomia salivary hypofunction chemotherapy 5-fluorouracil inflammation acupuncture |
| title | Protective Effect of Electroacupuncture on Chemotherapy-Induced Salivary Gland Hypofunction in a Mouse Model |
| title_full | Protective Effect of Electroacupuncture on Chemotherapy-Induced Salivary Gland Hypofunction in a Mouse Model |
| title_fullStr | Protective Effect of Electroacupuncture on Chemotherapy-Induced Salivary Gland Hypofunction in a Mouse Model |
| title_full_unstemmed | Protective Effect of Electroacupuncture on Chemotherapy-Induced Salivary Gland Hypofunction in a Mouse Model |
| title_short | Protective Effect of Electroacupuncture on Chemotherapy-Induced Salivary Gland Hypofunction in a Mouse Model |
| title_sort | protective effect of electroacupuncture on chemotherapy induced salivary gland hypofunction in a mouse model |
| topic | xerostomia salivary hypofunction chemotherapy 5-fluorouracil inflammation acupuncture |
| url | https://www.mdpi.com/1422-0067/24/14/11654 |
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