Bactericidal effect of a diode laser on Enterococcus faecalis in human primary teeth—an in vitro study
Abstract Background In recent years, the diode laser (810 nm) has been used for root canal disinfection, which plays an important role in endodontic therapy. This study was undertaken to evaluate the disinfecting ability of a diode laser in experimentally infected root canals of primary teeth. Metho...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-08-01
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| Series: | BMC Oral Health |
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| Online Access: | http://link.springer.com/article/10.1186/s12903-018-0611-6 |
| _version_ | 1811285991778942976 |
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| author | Shanshan Dai Gang Xiao Ning Dong Fei Liu Shuyang He Qingyu Guo |
| author_facet | Shanshan Dai Gang Xiao Ning Dong Fei Liu Shuyang He Qingyu Guo |
| author_sort | Shanshan Dai |
| collection | DOAJ |
| description | Abstract Background In recent years, the diode laser (810 nm) has been used for root canal disinfection, which plays an important role in endodontic therapy. This study was undertaken to evaluate the disinfecting ability of a diode laser in experimentally infected root canals of primary teeth. Methods Human retained mandibular primary anterior teeth without apical foramen resorption were selected and contaminated with Enterococcus faecalis for 21 days. The specimens were randomly divided into four groups: the negative group (no treatment), positive group (5.25% NaOCl), diode laser group (diode laser), and diode-NaOCl group (diode laser combined with NaOCl). The disinfecting abilities of the treatments were measured by the numbers of bacteria, scanning electron microscopy and confocal laser microscopy (live-dead staining). Results Eighty teeth were selected. After irradiation and irrigation, the elimination of bacteria and the smear layer in the laser groups and positive group were significantly superior, compared with the negative group (p < 0.01). In the diode-NaOCl group, bacterial reduction reached nearly 100% on the surfaces of root canals; live bacteria were rarely observed, even in deeper dentinal tubules. Conclusion Use of a diode laser, especially in combination with NaOCl, was effective for disinfecting infected root canals of primary teeth. |
| first_indexed | 2024-04-13T02:53:07Z |
| format | Article |
| id | doaj.art-4aad42e9dbe144be9b0a64c23220e785 |
| institution | Directory Open Access Journal |
| issn | 1472-6831 |
| language | English |
| last_indexed | 2024-04-13T02:53:07Z |
| publishDate | 2018-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Oral Health |
| spelling | doaj.art-4aad42e9dbe144be9b0a64c23220e7852022-12-22T03:05:47ZengBMCBMC Oral Health1472-68312018-08-011811710.1186/s12903-018-0611-6Bactericidal effect of a diode laser on Enterococcus faecalis in human primary teeth—an in vitro studyShanshan Dai0Gang Xiao1Ning Dong2Fei Liu3Shuyang He4Qingyu Guo5Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine ResearchKey Laboratory of Shaanxi Province for Craniofacial Precision Medicine ResearchKey Laboratory of Shaanxi Province for Craniofacial Precision Medicine ResearchKey Laboratory of Shaanxi Province for Craniofacial Precision Medicine ResearchKey Laboratory of Shaanxi Province for Craniofacial Precision Medicine ResearchKey Laboratory of Shaanxi Province for Craniofacial Precision Medicine ResearchAbstract Background In recent years, the diode laser (810 nm) has been used for root canal disinfection, which plays an important role in endodontic therapy. This study was undertaken to evaluate the disinfecting ability of a diode laser in experimentally infected root canals of primary teeth. Methods Human retained mandibular primary anterior teeth without apical foramen resorption were selected and contaminated with Enterococcus faecalis for 21 days. The specimens were randomly divided into four groups: the negative group (no treatment), positive group (5.25% NaOCl), diode laser group (diode laser), and diode-NaOCl group (diode laser combined with NaOCl). The disinfecting abilities of the treatments were measured by the numbers of bacteria, scanning electron microscopy and confocal laser microscopy (live-dead staining). Results Eighty teeth were selected. After irradiation and irrigation, the elimination of bacteria and the smear layer in the laser groups and positive group were significantly superior, compared with the negative group (p < 0.01). In the diode-NaOCl group, bacterial reduction reached nearly 100% on the surfaces of root canals; live bacteria were rarely observed, even in deeper dentinal tubules. Conclusion Use of a diode laser, especially in combination with NaOCl, was effective for disinfecting infected root canals of primary teeth.http://link.springer.com/article/10.1186/s12903-018-0611-6Diode laserPrimary teethDisinfectionEnterococcus faecalis |
| spellingShingle | Shanshan Dai Gang Xiao Ning Dong Fei Liu Shuyang He Qingyu Guo Bactericidal effect of a diode laser on Enterococcus faecalis in human primary teeth—an in vitro study BMC Oral Health Diode laser Primary teeth Disinfection Enterococcus faecalis |
| title | Bactericidal effect of a diode laser on Enterococcus faecalis in human primary teeth—an in vitro study |
| title_full | Bactericidal effect of a diode laser on Enterococcus faecalis in human primary teeth—an in vitro study |
| title_fullStr | Bactericidal effect of a diode laser on Enterococcus faecalis in human primary teeth—an in vitro study |
| title_full_unstemmed | Bactericidal effect of a diode laser on Enterococcus faecalis in human primary teeth—an in vitro study |
| title_short | Bactericidal effect of a diode laser on Enterococcus faecalis in human primary teeth—an in vitro study |
| title_sort | bactericidal effect of a diode laser on enterococcus faecalis in human primary teeth an in vitro study |
| topic | Diode laser Primary teeth Disinfection Enterococcus faecalis |
| url | http://link.springer.com/article/10.1186/s12903-018-0611-6 |
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