Alpha-1 Acid Glycoprotein and Podocin mRNA as Novel Biomarkers for Early Glomerular Injury in Obese Children
Introduction: Obesity, which is a serious problem in children, has a negative impact on many organs, including kidneys, and obesity-related glomerulopathy (ORG) is an increasingly common cause of ESKD (end-stage kidney disease) in adults. Early-detected and -treated glomerular lesions are reversible...
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MDPI AG
2021-09-01
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| Series: | Journal of Clinical Medicine |
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| Online Access: | https://www.mdpi.com/2077-0383/10/18/4129 |
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| author | Anna Medyńska Joanna Chrzanowska Katarzyna Kościelska-Kasprzak Dorota Bartoszek Marcelina Żabińska Danuta Zwolińska |
| author_facet | Anna Medyńska Joanna Chrzanowska Katarzyna Kościelska-Kasprzak Dorota Bartoszek Marcelina Żabińska Danuta Zwolińska |
| author_sort | Anna Medyńska |
| collection | DOAJ |
| description | Introduction: Obesity, which is a serious problem in children, has a negative impact on many organs, including kidneys, and obesity-related glomerulopathy (ORG) is an increasingly common cause of ESKD (end-stage kidney disease) in adults. Early-detected and -treated glomerular lesions are reversible, so it is important to find a useful marker of early damage. The study aimed to evaluate the albumin-to-creatinine ratio (ACR), urinary alpha-1-acid glycoprotein (α1-AGP), and mRNA of podocyte-specific proteins as indicators of glomerular injury and their relationship with the degree of obesity and metabolic disorders. Materials and Methods: A total of 125 obese children and 33 healthy peers were enrolled. Patients were divided into two groups, depending on SDS BMI values. ACR, α1-AGP, mRNA expression of nephrin, synaptopodin, podocin, and C2AP protein in urine sediment were measured. Results: ACR values did not differ between groups and were within the normal range. α1-AGP and mRNA expression were significantly higher in obese children compared with controls. mRNA expression of the remaining podocyte proteins was similar in both groups. No significant differences concerning all examined parameters were found depending on the degree of obesity. There was a positive significant correlation between α1-AGP and ACR. Conclusions: Increased α1-AGP before the onset of albuminuria suggests its usefulness as a biomarker of early glomerular damage in obese children. An increased podocin mRNA expression also indicates podocyte damage and may be linked to ORG development. The lack of increase in expression of other podocyte proteins suggests that podocin mRNA may be a more specific and sensitive biomarker. The degree of obesity has no impact on the tested parameters, but further studies are needed to confirm it. |
| first_indexed | 2024-03-10T07:33:45Z |
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| institution | Directory Open Access Journal |
| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-10T07:33:45Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Clinical Medicine |
| spelling | doaj.art-4aae4218a32349e6b43c32e01844cdcc2023-11-22T13:39:56ZengMDPI AGJournal of Clinical Medicine2077-03832021-09-011018412910.3390/jcm10184129Alpha-1 Acid Glycoprotein and Podocin mRNA as Novel Biomarkers for Early Glomerular Injury in Obese ChildrenAnna Medyńska0Joanna Chrzanowska1Katarzyna Kościelska-Kasprzak2Dorota Bartoszek3Marcelina Żabińska4Danuta Zwolińska5Department of Pediatric Nephrology, Wroclaw Medical University, 50-367 Wrocław, PolandDepartment of Pediatric Endocrinology and Diabetology, Wroclaw Medical University, 50-367 Wrocław, PolandSpecialist Laboratory at the Department of Nephrology and Transplantation Medicine, Wroclaw Medical University, 50-367 Wrocław, PolandSpecialist Laboratory at the Department of Nephrology and Transplantation Medicine, Wroclaw Medical University, 50-367 Wrocław, PolandSpecialist Laboratory at the Department of Nephrology and Transplantation Medicine, Wroclaw Medical University, 50-367 Wrocław, PolandDepartment of Pediatric Nephrology, Wroclaw Medical University, 50-367 Wrocław, PolandIntroduction: Obesity, which is a serious problem in children, has a negative impact on many organs, including kidneys, and obesity-related glomerulopathy (ORG) is an increasingly common cause of ESKD (end-stage kidney disease) in adults. Early-detected and -treated glomerular lesions are reversible, so it is important to find a useful marker of early damage. The study aimed to evaluate the albumin-to-creatinine ratio (ACR), urinary alpha-1-acid glycoprotein (α1-AGP), and mRNA of podocyte-specific proteins as indicators of glomerular injury and their relationship with the degree of obesity and metabolic disorders. Materials and Methods: A total of 125 obese children and 33 healthy peers were enrolled. Patients were divided into two groups, depending on SDS BMI values. ACR, α1-AGP, mRNA expression of nephrin, synaptopodin, podocin, and C2AP protein in urine sediment were measured. Results: ACR values did not differ between groups and were within the normal range. α1-AGP and mRNA expression were significantly higher in obese children compared with controls. mRNA expression of the remaining podocyte proteins was similar in both groups. No significant differences concerning all examined parameters were found depending on the degree of obesity. There was a positive significant correlation between α1-AGP and ACR. Conclusions: Increased α1-AGP before the onset of albuminuria suggests its usefulness as a biomarker of early glomerular damage in obese children. An increased podocin mRNA expression also indicates podocyte damage and may be linked to ORG development. The lack of increase in expression of other podocyte proteins suggests that podocin mRNA may be a more specific and sensitive biomarker. The degree of obesity has no impact on the tested parameters, but further studies are needed to confirm it.https://www.mdpi.com/2077-0383/10/18/4129obesityglomerular injuryalpha-1 acid glycoproteinurinary mRNA expression of podocyte-associated proteins |
| spellingShingle | Anna Medyńska Joanna Chrzanowska Katarzyna Kościelska-Kasprzak Dorota Bartoszek Marcelina Żabińska Danuta Zwolińska Alpha-1 Acid Glycoprotein and Podocin mRNA as Novel Biomarkers for Early Glomerular Injury in Obese Children Journal of Clinical Medicine obesity glomerular injury alpha-1 acid glycoprotein urinary mRNA expression of podocyte-associated proteins |
| title | Alpha-1 Acid Glycoprotein and Podocin mRNA as Novel Biomarkers for Early Glomerular Injury in Obese Children |
| title_full | Alpha-1 Acid Glycoprotein and Podocin mRNA as Novel Biomarkers for Early Glomerular Injury in Obese Children |
| title_fullStr | Alpha-1 Acid Glycoprotein and Podocin mRNA as Novel Biomarkers for Early Glomerular Injury in Obese Children |
| title_full_unstemmed | Alpha-1 Acid Glycoprotein and Podocin mRNA as Novel Biomarkers for Early Glomerular Injury in Obese Children |
| title_short | Alpha-1 Acid Glycoprotein and Podocin mRNA as Novel Biomarkers for Early Glomerular Injury in Obese Children |
| title_sort | alpha 1 acid glycoprotein and podocin mrna as novel biomarkers for early glomerular injury in obese children |
| topic | obesity glomerular injury alpha-1 acid glycoprotein urinary mRNA expression of podocyte-associated proteins |
| url | https://www.mdpi.com/2077-0383/10/18/4129 |
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