In vitro modeling of myocardial ischemia/reperfusion injury with murine or human 3D cardiac spheroids
Summary: Myocardial infarction (MI) is the primary cause of death worldwide, but there are no clinically relevant models to study MI. Here, we describe an ischemia/reperfusion (I/R) injury model typical of MI using mouse or human 3D in vitro cardiac spheroids (CSs). First, we demonstrated the cultur...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722006311 |
| _version_ | 1811183855526215680 |
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| author | Poonam Sharma Clara Liu Chung Ming Carmine Gentile |
| author_facet | Poonam Sharma Clara Liu Chung Ming Carmine Gentile |
| author_sort | Poonam Sharma |
| collection | DOAJ |
| description | Summary: Myocardial infarction (MI) is the primary cause of death worldwide, but there are no clinically relevant models to study MI. Here, we describe an ischemia/reperfusion (I/R) injury model typical of MI using mouse or human 3D in vitro cardiac spheroids (CSs). First, we demonstrated the culture and maintenance of CSs. Then, we detailed how to expose CSs to pathophysiological oxygen concentrations to induce I/R injury. The protocol can be used in combination with viability, contractility, and mRNA expression level measurements.For complete details on the use and execution of this protocol, please refer to Sharma et al. (2022). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-11T13:03:36Z |
| format | Article |
| id | doaj.art-4ab0a9cb608448a3903a896a33c91994 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-11T13:03:36Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-4ab0a9cb608448a3903a896a33c919942022-12-22T04:22:50ZengElsevierSTAR Protocols2666-16672022-12-0134101751In vitro modeling of myocardial ischemia/reperfusion injury with murine or human 3D cardiac spheroidsPoonam Sharma0Clara Liu Chung Ming1Carmine Gentile2The University of Newcastle, Newcastle, NSW 2308, Australia; University of Sydney, Sydney, NSW 2000, Australia; Kolling Institute of Medical Research, Royal North Shore Hospital, Sydney, NSW 2065, Australia; University of Technology Sydney, Sydney, NSW 2007, Australia; Corresponding authorUniversity of Technology Sydney, Sydney, NSW 2007, AustraliaUniversity of Sydney, Sydney, NSW 2000, Australia; Kolling Institute of Medical Research, Royal North Shore Hospital, Sydney, NSW 2065, Australia; University of Technology Sydney, Sydney, NSW 2007, Australia; Corresponding authorSummary: Myocardial infarction (MI) is the primary cause of death worldwide, but there are no clinically relevant models to study MI. Here, we describe an ischemia/reperfusion (I/R) injury model typical of MI using mouse or human 3D in vitro cardiac spheroids (CSs). First, we demonstrated the culture and maintenance of CSs. Then, we detailed how to expose CSs to pathophysiological oxygen concentrations to induce I/R injury. The protocol can be used in combination with viability, contractility, and mRNA expression level measurements.For complete details on the use and execution of this protocol, please refer to Sharma et al. (2022). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166722006311Health sciencesOrganoidsTissue engineering |
| spellingShingle | Poonam Sharma Clara Liu Chung Ming Carmine Gentile In vitro modeling of myocardial ischemia/reperfusion injury with murine or human 3D cardiac spheroids STAR Protocols Health sciences Organoids Tissue engineering |
| title | In vitro modeling of myocardial ischemia/reperfusion injury with murine or human 3D cardiac spheroids |
| title_full | In vitro modeling of myocardial ischemia/reperfusion injury with murine or human 3D cardiac spheroids |
| title_fullStr | In vitro modeling of myocardial ischemia/reperfusion injury with murine or human 3D cardiac spheroids |
| title_full_unstemmed | In vitro modeling of myocardial ischemia/reperfusion injury with murine or human 3D cardiac spheroids |
| title_short | In vitro modeling of myocardial ischemia/reperfusion injury with murine or human 3D cardiac spheroids |
| title_sort | in vitro modeling of myocardial ischemia reperfusion injury with murine or human 3d cardiac spheroids |
| topic | Health sciences Organoids Tissue engineering |
| url | http://www.sciencedirect.com/science/article/pii/S2666166722006311 |
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